A5080591515- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- HER2/EGFR in Cancer Research
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Schizophrenia research and treatment
- Toxin Mechanisms and Immunotoxins
- RNA modifications and cancer
- Renal and related cancers
- Kruppel-like factors research
- Bipolar Disorder and Treatment
- Bone and Dental Protein Studies
- Breast Cancer Treatment Studies
- Endoplasmic Reticulum Stress and Disease
- Electroconvulsive Therapy Studies
- Protein purification and stability
- Cytokine Signaling Pathways and Interactions
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- Advanced Biosensing Techniques and Applications
Fondazione IRCCS Istituto Nazionale dei Tumori
2013-2024
University of Milan
1999-2013
Ingenious Targeting Laboratory (United States)
2013
Fudan University Shanghai Cancer Center
2013
Policlinico San Matteo Fondazione
2001
Istituti di Ricovero e Cura a Carattere Scientifico
2001
Target (United States)
2000
University of Pavia
1990-1998
We have designed a set of oligonucleotide primers to amplify the cDNA mouse immunoglobulin heavy and light chain variable domains by polymerase reaction. The incorporate restriction sites that allow be force-cloned for sequencing expression. Here we applied technique clone sequence five hybridoma antibodies express mouse-human chimeric antibody binds human mammary carcinoma line MCF-7. should also lead cloning antigen-binding specificities directly from genes.
Prediction of the clinical outcome breast cancer is multi-faceted and challenging. There growing evidence that complexity tumour micro-environment, consisting several cell types a complex mixture proteins, plays an important role in development, progression, response to therapy. In current study, we investigated whether invasive tumours can be classified on basis expression extracellular matrix (ECM) components such classification representative different outcomes. We first examined...
Abstract BACKGROUND To the authors' knowledge, no effective medical therapy currently is available for advanced chordoma. Imatinib mesylate a tyrosine kinase inhibitor targeting platelet‐derived growth factor receptor‐β (PDGFRB), BCR‐ABL, and KIT. METHODS Six patients with chordoma were treated imatinib at dose of 800 mg daily. In all patients, tumor was found to be positive PDGFRB, in four PDGFRB shown phosphorylated/expressed. RESULTS After treatment period ≥ 1 year, overt liquefaction...
We previously selected a panel of 3 breast cancer biomarkers (BC1, BC2, and BC3) from serum samples collected at single hospital based on their collective contribution to the optimal separation patients noncancer controls by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The identities general applicability these markers, however, were unknown. In this study, we performed protein expression profiling obtained second hospital, included greater...
The extracellular matrix (ECM) contributes to the biological and clinical heterogeneity of breast cancer, different prognostic groups can be identified according specific ECM signatures. In high-grade, but not low-grade, tumors, an signature characterized by high SPARC expression (ECM3) identifies tumors with increased epithelial-to-mesenchymal transition (EMT), reduced treatment response, poor prognosis. To better understand how this ECM3 is contributing tumorigenesis, we expressed in...
Abstract Organization of cancer cells into endothelial-like cell-lined structures to support neovascularization and fuel solid tumors is a hallmark progression poor outcome. In triple-negative breast (TNBC), PDGFRβ has been identified as key player this process considered promising target for therapy. Thus, we aimed at investigating the role miRNAs therapeutic approach inhibit PDGFRβ-mediated vasculogenic properties TNBC, focusing on miR-9 miR-200. MDA-MB-231 MDA-MB-157 TNBC cell lines,...
Changes in amount and composition of extracellular matrix (ECM) are considered a hallmark tumor development. We tested the hypothesis that abnormal production ECM components leads to blood-released molecules representing circulating biomarkers. Candidate genes were selected through class comparison two publicly available datasets confirmed paired normal associated fibroblasts from breast carcinoma (BC) specimens. Production release evaluated human dermal (NHDFs) treated with conditioned...
Abstract Introduction Metastasis represents a major adverse step in the progression of breast carcinoma. Lymph node invasion is most relevant prognostic factor; however little known on molecular events associated with lymph metastasis process. This study to investigate status and role methylation metastatic tumors. Materials methods Bisulfite pyrosequencing used screen 6 putative tumor suppressor genes ( HIN-1, RASSF1A, RIL, CDH13, RARβ2 E-cadherin) 38 pairs primary tumors metastases....
HER2-overexpression promotes malignancy by modulating signalling molecules, which include PTPs/DSPs (protein tyrosine and dual-specificity phosphatases). Our aim was to identify displaying HER2-associated expression alterations.HER2 activity modulated in MDA-MB-453 cells analysed with a DNA oligoarray, RT-PCR immunoblotting. Two public breast tumor datasets were differentially expressed HER2-positive tumors.In (1) HER2-inhibition up-regulated 4 PTPs (PTPRA, PTPRK, PTPN11, PTPN18) 11 DSPs (7...
We recently showed that differential expression of extracellular matrix (ECM) genes delineates four subgroups breast carcinomas (ECM1, -2, -3- and -4) with different clinical outcome. To further investigate the characteristics ECM signature its impact on tumor progression, we conducted unsupervised clustering analyses in 6 additional independent datasets invasive tumors from platforms for a total 643 samples. Use algorithms identified ECM3 as an group all tested. homogeneous gene pattern,...
Breath analysis represents a new frontier in medical diagnosis and powerful tool for cancer biomarker discovery due to the recent development of analytical platforms detection identification human exhaled volatile compounds. Statistical bioinformatic tools may represent an effective complement technical instrumental enhancements needed fully exploit clinical applications breath analysis. Our exploratory study cohort 14 breast patients 11 healthy volunteers used secondary electrospray...
The recent dramatic increase in breast cancer incidence across China with progressive urbanization and economic development has signaled the urgent need for molecular clinical detailing of Chinese population. Our analyses a unique transethnic collection frozen specimens from Shanghai Fudan Cancer Center (Chinese Han) profiled simultaneously an analogous Caucasian Italian series revealed consistent transcriptomic data lacking batch effects. prevalence Luminal A subtype was significantly lower...
Abstract Background: Case–control studies show that copper (Cu) is high and zinc (Zn) low in blood urine of women with breast cancer compared controls. Methods: To assess whether prediagnostic Cu Zn are associated risk, OR according to Cu, Zn, Cu/Zn ratio plasma was estimated a nested case–control study within the ORDET cohort, using conditional logistic regression adjusted for multiple variables: First 496 cases matched controls, diagnosed ≥2 years after recruitment (to eliminate reverse...
Background : HER2-overexpression promotes malignancy by modulating signalling molecules, which include PTPs/DSPs (protein tyrosine and dual-specificity phosphatases). Our aim was to identify displaying HER2-associated expression alterations. Methods HER2 activity modulated in MDA-MB-453 cells analysed with a DNA oligoarray, RT-PCR immunoblotting. Two public breast tumor datasets were differentially expressed HER2-positive tumors. Results In (1) HER2-inhibition up-regulated 4 PTPs (PTPRA,...
We have previously reported hepcidin and ferritin increases in the plasma of breast cancer patients, but not patients with benign disease. hypothesized that these differences systemic iron homeostasis may reflect alterations different iron-related proteins also play a key biochemical regulatory role cancer. Thus, here we explored expression bundle molecules involved both tumorigenesis tissue samples. Enzyme-linked immunosorbent assay (ELISA) or reverse-phase protein array (RPPA), were used...
Hepcidin-25 production is stimulated by systemic inflammation, and it interferes with iron utilization, leading to anemia. This study aimed investigate the relationships between plasma levels of hepcidin, interleukin-6 (IL-6), erythropoietin (EPO) erythroferrone (ERFE) in patients benign breast disease or cancer.Plasma samples from a cohort 131 (47 84 cancer) were subjected evaluation IL-6, EPO ERFE using SELDI-TOF-MS immunoassays.An elevated hepcidin was observed malignant tumors compared...
Abstract Several specific alterations of the extracellular matrix can be considered a distinctive hallmark cancer. In particular, different morphology collagen scaffold is frequently found within peritumoural environment. this study, we report about significant difference in ultrastructural organization at supra‐molecular level between perilesional and tumour area human breast carcinoma samples. demonstrated that polarization‐resolved second‐harmonic generation (P‐SHG) microscopy able to...
Twenty-four chronic schizophrenic outpatients with a mean age of 37.21 years +/- 9.96 SD were treated risperidone (RSP) at the dosage 2-9 mg/die (mean 4.46 1.30 SD, 0.06 mg/kg 0.01 SD) for year. Clinical evaluation was assessed Brief Psychiatric Rating Scale (BPRS), Positive and Negative Symptoms (PANSS), Extrapyramidal Side Effects (EPSE) checklist Anticholinergic (ACS) T0, then after 1 (T1), 2 (T2), 3 (T3), 6 (T6), 9 (T9) 12 (T12) months. RSP 9-hydroxy-risperidone (9OH-RSP) plasma levels...