A5022962128- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Air Quality and Health Impacts
- Respiratory and Cough-Related Research
- Neonatal Respiratory Health Research
- Neuroscience of respiration and sleep
- Bacterial Identification and Susceptibility Testing
- Obstructive Sleep Apnea Research
- Analytical chemistry methods development
- Asthma and respiratory diseases
- Biosensors and Analytical Detection
- Biochemical Analysis and Sensing Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Noise Effects and Management
- Ion channel regulation and function
- Cardiovascular and exercise physiology
- Energy and Environment Impacts
- Inhalation and Respiratory Drug Delivery
- Cystic Fibrosis Research Advances
- Diet and metabolism studies
- Microbial Metabolic Engineering and Bioproduction
- Epilepsy research and treatment
University Children’s Hospital Basel
2017-2025
University of Basel
2017-2025
Infant
2021
ETH Zurich
2012-2019
École Polytechnique Fédérale de Lausanne
2015-2019
National Research Council
2009-2015
Institute of Biomedical Technologies
2011-2015
National Academies of Sciences, Engineering, and Medicine
2011-2014
Circadian clocks play a significant role in the correct timing of physiological metabolism, and clock disruption might lead to pathological changes metabolism. One interesting method assess current state metabolism is metabolomics. Metabolomics tries capture entirety small molecules, i.e. building blocks given matrix, such as blood, saliva or urine. Using mass spectrometric approaches we others have shown that portion human metabolome blood exhibits circadian modulation; independent food...
Despite the attractiveness of breath analysis as a non-invasive means to retrieve relevant metabolic information, its introduction into routine clinical practice remains challenge. Among all different analytical techniques available interrogate exhaled breath, secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) offers number advantages (e.g., real-time, yet wide, metabolome coverage) that makes it ideal for untargeted and targeted studies. However, so far,...
Online breath analysis is an attractive approach to track exhaled compounds without sample preparation. Current commercially available real-time platforms require the purchase of a full mass spectrometer. Here we present ion source compatible with virtually any preexisting atmospheric pressure ionization spectrometer that allows breath. We illustrate capabilities such technological development by upgrading orbitrap As result, detected in between 70 and 900 Da, accuracy typically <1 ppm;...
ABSTRACT Background and objective Diagnosis of idiopathic pulmonary fibrosis (IPF) is complex its pathogenesis poorly understood. Recent findings indicate elevated levels proline other amino acids in lung tissue IPF patients which may also be diagnostic value. Following these findings, we hypothesized that such altered metabolic profiles would mirrored exhaled breath could therefore captured non‐invasively real time. Methods We aimed to validate results using real‐time analysis by secondary...
The metabolic phenotype varies widely due to external factors such as diet and gut microbiome composition, among others. Despite these temporal fluctuations, urine metabolite profiling studies have suggested that there are highly individual phenotypes persist over extended periods of time. This hypothesis was tested by analyzing the exhaled breath a group subjects during nine days mass spectrometry. Consistent with previous metabolomic based on urine, we conclude signatures composition...
<b><i>Background:</i></b> It has been suggested that exhaled breath contains relevant information on health status. <b><i>Objectives:</i></b> We hypothesized a novel mass spectrometry (MS) technique to analyze in real time could be useful differentiate breathprints from chronic obstructive pulmonary disease (COPD) patients and controls (smokers nonsmokers). <b><i>Methods:</i></b> studied 61 participants including 25 COPD...
The development of noninvasive analytical techniques is interest to the field chronobiology, in order reveal human metabolome that seems show temporal patterns and predict internal body time. We report on real-time mass spectrometric analysis breath as a potential method be used this field. 12 subjects was analyzed during 9 days by secondary electrospray ionization-mass spectrometry (SESI-MS). samples were collected four time slots: morning (8:00-11:00), before lunch (11:00-13:00), after...
Abstract Noninvasive, real‐time pharmacokinetic (PK) monitoring of ketamine, propofol, and valproic acid, their metabolites was achieved in mice, using secondary electrospray ionization high‐resolution mass spectrometry. The PK profile a drug influences its efficacy toxicity because it determines exposure time levels. antidepressant anaesthetic ketamine (Ket) four Ket were studied detail simultaneously determined following application different sub‐anaesthetic doses Ket. Bioavailability...
<h3>Background</h3> Obstructive sleep apnoea (OSA) is highly prevalent and associated with cardiovascular metabolic changes. OSA usually diagnosed by polysomnography which time-consuming provides little information on the patient9s phenotype thus limiting a personalised treatment approach. Exhaled breath contains metabolism can be analysed mass spectrometry within minutes. The objective of this study was to identify profile in recurrence use secondary-electrospray-ionization-mass (SESI-MS)....
In recent years, breath analysis in real time has become a noninvasive alternative for the diagnosis of diseases and molecular fingerprinting exhaled breath. However, techniques used lack capabilities proper identification compounds found exhalome. Here, we report use UHPLC-HRMS as tool several aldehydes (2-alkenals, 4-hydroxy-2-alkenals, 4-hydroxy-2,6-alkadienals), biomarkers lipid peroxidation, condensate three healthy subjects (N = 3). Some studied have never been identified before. Their...
The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathway for cellular respiration in aerobic organisms. It provides and collects intermediates many other interconnecting pathways acts as a hub connecting metabolism carbohydrates, fatty acids, amino acids. Alteration intracellular levels its has been linked with wide range illnesses ranging from cancer to necrosis or liver cirrhosis. Therefore, there exists an intrinsic interest monitoring such metabolites. Our goal...
Sampling of volatile organic compounds (VOCs) has shown promise for detection a range diseases but results have proved hard to replicate due lack standardization. In this work we introduce the 'Peppermint Initiative'. The initiative seeks disseminate standardized experiment that allows comparison breath sampling and data analysis methods. Further, it share set benchmark values measurement VOCs in breath. Pilot are presented illustrate approach interpretation obtained from Peppermint...
Sleep is crucial to restore body functions and metabolism across nearly all tissues cells, sleep restriction linked various metabolic dysfunctions in humans. Using exhaled breath analysis by secondary electrospray ionization high-resolution mass spectrometry, we measured the human metabolome at 10-s resolution a night of combination with conventional polysomnography. Our subsequent almost 2,000 metabolite features demonstrates rapid, reversible control major pathways individual vigilance...
<title>Abstract</title> The objectification of pain presents a significant clinical challenge, particularly in children, elderly individuals, patients with disabilities and unconscious patients. It is critically important to accurately assess these populations due the heightened risk undertreatment. Using cold pressor test (CPT) as induction model, we combined real-time breath metabolomics pathway analysis uncover metabolic shifts. Exhaled was analyzed discovery cohort (n=19) validated an...
Real-time breath analysis has shown potential as a non-invasive method for detecting oxidative stress and airway inflammation. However, there is lack of data on the association full-breath profiles with established urinary biomarkers respiratory inflammation, which could help advance implementation this in clinical practice. We analyzed 25 tobacco smoke-exposed 103 non-exposed children via real-time secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) determined...
Background Air pollution increases inflammation and reactive oxygen species that can induce autophagy, thereby leading to airway remodelling. However, it is unclear whether prenatal air may impact proteins involved in autophagy Objectives To investigate the associations of with indicative senescence, remodelling infants. Methods We included 387 healthy term newborns from BILD cohort study measured 11 proteins: IL-1β, IL-8, MMP-3, MMP-9, PDGF-AA, TGF-β, sirtuin-1, p62, LC3B, TNF-α, Beclin-1...
Omega-oxidation is a fatty acid degradation pathway that can occur alternatively to the dominant β-oxidation. The dysregulation of oxidation has been related with variety diseases, termed disorders. This work shows evidence for real-time detection in exhaled breath complete series saturated linear ω-hydroxyalkanoic acids, ω-oxoalkanoic and alkanedioic acids carbon chain lengths 5–15. We present comprehensive analytical workflow using online subsequent offline methods: secondary electrospray...
The proton transfer reaction in secondary electrospray ionization ultimately proceeds with gas-phase ions.
Rationale: Infants born prematurely have impaired capacity to deal with oxidative stress shortly after birth. Objectives: We hypothesize that the relative impact of exposure air pollution on lung function is higher in preterm than term infants. Methods: In prospective BILD (Basel-Bern Infant Lung Development) birth cohort 254 and 517 infants, we investigated associations particulate matter ⩽10 μm aerodynamic diameter (PM10) nitrogen dioxide at 44 weeks' postconceptional age exhaled markers...
Journal Article Cohort Profile Update: The Bern Basel Infant Lung Development Get access Yasmin Salem, Salem Division of Pediatric Respiratory Medicine and Allergology, Department Pediatrics, Inselspital, University Hospital, Bern, SwitzerlandGraduate School for Health Sciences, Switzerland https://orcid.org/0000-0001-8731-0885 Search other works by this author on: Oxford Academic PubMed Google Scholar Julian Jakob, Jakob SwitzerlandInstitute Primary Care (BIHAM), Ruth Steinberg, Steinberg...