A5060019261- Lymphatic System and Diseases
- Angiogenesis and VEGF in Cancer
- Medical Imaging Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Radiomics and Machine Learning in Medical Imaging
- Sympathectomy and Hyperhidrosis Treatments
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Dermatologic Treatments and Research
- Cell Adhesion Molecules Research
- Lymphatic Disorders and Treatments
- Cutaneous Melanoma Detection and Management
- Vascular Malformations and Hemangiomas
- Skin Protection and Aging
- Esophageal Cancer Research and Treatment
- Psoriasis: Treatment and Pathogenesis
- Body Contouring and Surgery
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Skin and Cellular Biology Research
- Hair Growth and Disorders
- Dermatology and Skin Diseases
- RNA Research and Splicing
- Wound Healing and Treatments
- CNS Lymphoma Diagnosis and Treatment
ETH Zurich
2015-2024
École Polytechnique Fédérale de Lausanne
2012-2023
Board of the Swiss Federal Institutes of Technology
2013-2022
Council of Science Editors
2017
University of Birmingham
2016
Massachusetts General Hospital
1999-2014
Harvard University
2002-2014
University Hospital of Zurich
2012
Universitätsklinikum Erlangen
2012
The University of Queensland
2012
The central nervous system (CNS) is considered an organ devoid of lymphatic vasculature. Yet, part the cerebrospinal fluid (CSF) drains into cervical lymph nodes (LNs). mechanism CSF entry LNs has been unclear. Here we report surprising finding a vessel network in dura mater mouse brain. We show that dural vessels absorb from adjacent subarachnoid space and brain interstitial (ISF) via glymphatic system. Dural transport deep (dcLNs) foramina at base skull. In transgenic model expressing...
Abstract Objective MicroRNAs (miRNA) have recently emerged as a new class of modulators gene expression. In this study we investigated the expression, regulation, and function miR‐155 miR‐146a in rheumatoid arthritis (RA) synovial fibroblasts (RASFs) RA tissue. Methods Locked nucleic acid microarray was used to screen for differentially expressed miRNA RASFs treated with tumor necrosis factor α (TNFα). TaqMan‐based real‐time polymerase chain reaction applied measure levels miR‐146a. Enforced...
The mechanisms of tumor metastasis to the sentinel lymph nodes are poorly understood. Vascular endothelial growth factor (VEGF)-A plays a principle role in progression and angiogenesis; however, its tumor-associated lymphangiogenesis lymphatic has remained unclear. We created transgenic mice that overexpress VEGF-A green fluorescent protein specifically skin, subjected them standard chemically-induced skin carcinogenesis regimen. found not only strongly promotes multistep carcinogenesis, but...
Psoriatic skin is characterized by microvascular hyperpermeability and angioproliferation, but the mechanisms responsible are unknown. We report here that hyperplastic epidermis of psoriatic expresses strikingly increased amounts vascular permeability factor (VPF; endothelial growth factor), a selective cell mitogen enhances permeability. Moreover, two VPF receptors, kdr flt-1, overexpressed papillary dermal cells. Transforming alpha (TGF-alpha), cytokine also in epidermis, induced gene...
The murine hair follicle undergoes pronounced cyclic expansion and regression, leading to rapidly changing demands for its vascular support. Our study aimed quantify the changes of perifollicular vascularization characterize biological role VEGF growth, angiogenesis, cycling. We found a significant increase in during growth phase (anagen) cycle, followed by regression angiogenic blood vessels involution (catagen) resting (telogen) phase. Perifollicular angiogenesis was temporally spatially...
Cell migration plays a major role in development, physiology, and disease, is frequently evaluated vitro by the monolayer wound healing assay. The assay analysis, however, time-consuming task that often performed manually. In order to accelerate this we have developed TScratch, new, freely available image analysis technique associated software tool uses fast discrete curvelet transform automate measurement of area occupied cells images. This helps significantly reduce time needed for enables...
Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses....
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF, VEGF-A) is a multifunctional cytokine with important roles in pathological angiogenesis. Using an adenoviral vector engineered to express murine VEGF-A(164), we previously investigated the steps and mechanisms by which this induced formation of new blood vessels adult immunodeficient mice demonstrated that newly formed closely resembled those found VEGF-A-expressing tumors. We now report that, addition inducing...
Cerebrospinal fluid (CSF) has been commonly accepted to drain through arachnoid projections from the subarachnoid space dural venous sinuses. However, a lymphatic component CSF outflow long known. Here, we utilize lymphatic-reporter mice and high-resolution stereomicroscopy characterize anatomical routes dynamics of CSF. After infusion into lateral ventricle, tracers spread paravascular spaces pia mater cortex brain. Tracers also rapidly reach lymph nodes using perineural foramina in skull....
Abstract Early during development, one of the first indications that lymphangiogenesis has begun is polarized expression homeobox gene Prox1 in a subpopulation venous endothelial cells. It been shown previously cardinal vein promotes and maintains budding cells will form lymphatic vascular system. ‐deficient mice are devoid vasculature, these animals fail to acquire phenotype; instead, they remain as blood endothelium. To investigate whether sufficient induce fate endothelium, cDNA was...
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor, is a cytokine of central importance for the angiogenesis associated with cancers and other pathologies. Because often involves cell (EC) migration proliferation within collagen-rich extracellular matrix, we investigated possibility that VEGF promotes neovascularization through regulation collagen receptor expression. induced 5- to 7-fold increase in dermal microvascular EC surface protein expression two...