A5065860849- Angiogenesis and VEGF in Cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Coronary Interventions and Diagnostics
- RNA modifications and cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Signaling Pathways in Disease
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- Cardiac Ischemia and Reperfusion
- Genomics and Chromatin Dynamics
- Kruppel-like factors research
- Platelet Disorders and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Fibroblast Growth Factor Research
- Nuclear Receptors and Signaling
- Cardiac Fibrosis and Remodeling
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Proteoglycans and glycosaminoglycans research
- RNA and protein synthesis mechanisms
UNSW Sydney
2016-2025
Tiande (China)
2018
Prince of Wales Hospital
2002-2014
The University of Sydney
2004-2013
Royal North Shore Hospital
2013
Skin Cancer Foundation
2012
Victor Chang Cardiac Research Institute
2012
Ross School
2012
Target (United States)
2012
The Heart Research Institute
2000-2010
Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses....
A number of pathophysiologically relevant genes, including platelet-derived growth factor B-chain (PDGF-B), are induced in the vasculature after acute mechanical injury. In rat aorta, activated expression these genes was preceded by a marked increase amount early-growth-response gene product Egr-1 at endothelial wound edge. interacts with novel element proximal PDGF-B promoter, as well consensus elements promoters other This interaction is crucial for injury-induced promoter-dependent...
Catalytic DNA molecules that target the transcription factor c- jun inhibit skin cancer growth in mice.
Abstract In the FANTOM5 project, transcription initiation events across human and mouse genomes were mapped at a single base-pair resolution their frequencies monitored by CAGE (Cap Analysis of Gene Expression) coupled with single-molecule sequencing. Approximately three thousands samples, consisting variety primary cells, tissues, cell lines, time series samples during activation development, subjected to uniform pipeline data production. The analysis started measuring RNA extracts assess...
Hemodynamic forces, such as fluid shear stress, that act on the endothelial lining of cardiovascular system can modulate expression an expanding number genes crucial for homeostasis and pathogenesis vascular disease. A 6-bp core element (5'-GAGACC-3'), defined previously a shear-stress response is present in promoters many genes, including PDGF B-chain, whose modulated by stress. The identity nuclear protein(s) binding to this has not yet been elucidated. Using electrophoretic mobility shift...
The platelet-derived growth factor (PDGF) A-chain has been implicated in the initiation and progression of vascular occlusive lesions. elements human PDGF-A promoter that mediate increased expression gene endothelial cells have not identified. A potent inducer is phorbol 12-myristate 13-acetate (PMA). 5′-Deletion transfection analysis revealed a G+C-rich region proximal required for PMA-inducible expression. This bears overlapping consensus recognition sequences Sp1 Egr-1. PMA induces Egr-1...
Abstract Exposure of vascular endothelial cells to fluid mechanical forces can modulate the expression many genes involved in physiology and pathophysiology. Here, we report that platelet-derived growth factor (PDGF) A-chain gene is induced at level transcription cultured bovine aortic exposed a physiologic steady laminar shear stress (10 dyn/cm 2 ). 5′ Deletion analysis human PDGF-A promoter revealed GC-rich region near TATA box was required for shear-inducible reporter expression. This...
Surface binding of galectin family members has the potential to link distinct glycan structures growth regulation. Therefore, we addressed antiproliferative galectin-1 (Gal-1) in a panel carcinoma cell lines. We discovered inhibition by Gal-1 epithelial tumor lines from different origins and provide evidence that this effect requires functional interaction with alpha5beta1 integrin. Antiproliferative effects result Ras-MEK-ERK pathway consecutive transcriptional induction p27. have further...
Background: The basic region-leucine zipper protein c-Jun has been linked to cell proliferation, transformation, and apoptosis. However, a direct role for in angiogenesis not shown. Methods: We used human microvascular endothelial cells (HMEC-1) transfected with DNAzyme targeting the mRNA (Dz13), related oligonucleotides, or vehicle vitro models of migration, chemoinvasion, tubule formation, rat model corneal neovascularization, mouse solid tumor growth vascular factor (VEGF)–induced...
Abstract Macrophage migration inhibitory factor (MIF) has been shown to promote leukocyte–endothelial cell interactions, although whether this occurs via an effect on endothelial function remains unclear. Therefore, the aims of study were examine ability MIF expressed by cells leukocyte adhesion and investigate exogenous interactions. Using small interfering RNA inhibit HUVEC production, we found that deficiency reduced TNF-stimulated HUVECs support rolling under flow conditions. These...