A5027843897- Neuroblastoma Research and Treatments
- Protein Degradation and Inhibitors
- Muscle Physiology and Disorders
- Vitamin D Research Studies
- Reflective Practices in Education
- Estrogen and related hormone effects
- Nutrition and Health in Aging
- Innovations in Medical Education
- GDF15 and Related Biomarkers
- Retinoids in leukemia and cellular processes
- Biotin and Related Studies
- Ubiquitin and proteasome pathways
- Evaluation of Teaching Practices
- Biomedical and Engineering Education
- COVID-19 and Mental Health
- Chromatin Remodeling and Cancer
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Liver Diseases and Immunity
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Higher Education Learning Practices
- Bone Metabolism and Diseases
- Hereditary Neurological Disorders
UNSW Sydney
2015-2024
University College London
2017-2018
Royal Free London NHS Foundation Trust
2018
The Royal Free Hospital
2017
Blacktown & Mount Druitt Hospital
2015
Garvan Institute of Medical Research
1996-2014
Children's Cancer Institute Australia
2014
University of Bologna
2014
Sydney Children's Hospital
2014
The University of Sydney
2012
Background Patients with neuroblastoma due to the amplification of a 130-kb genomic DNA region containing MYCN oncogene have poor prognoses.
COVID-19 has had a profound influence on the conduct of teaching and learning in higher education. Almost everywhere sudden shift occurred as educators transitioned courses from mainly face-to-face to emergency remote instruction, mostly conducted online. While details varied for individual faculty members, institutions, countries, all confronted new challenges. We examine immediate effects Our results are based sample 309 courses, academic staff who taught them, at eight colleges...
The vitamin D receptor (VDR) is a transcription factor that transmits incoming 1,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3)) signaling via combined contact with coactivator proteins and specific DNA binding sites (VDREs), which ultimately results in activation of transcription. In contrast, the mechanisms transcriptional repression VDR are less well understood. This study documents VDR-dependent largely histone deacetylase (HDAC) activity. Direct, ligand-sensitive protein-protein...
Myc oncoproteins exert tumorigenic effects by regulating expression of target oncogenes. Histone H3 lysine 79 (H3K79) methylation at Myc-responsive elements gene promoters is a strict prerequisite for Myc-induced transcriptional activation, and DOT1L the only known histone methyltransferase that catalyzes H3K79 methylation. Here, we show N-Myc upregulates mRNA protein binding to promoter. shRNA-mediated depletion reduced genes ODC1 E2F2 bound Box II domain protein, knockdown neuroblastoma...
Aging | doi:10.18632/aging.100436. Angie M. Y. Shum, Theodore Mahendradatta, Ryland J. Taylor, Arran B. Painter, Melissa Moore, Maria Tsoli, Timothy C. Tan, Stephen Clarke, Graham R. Robertson, Patsie Polly
Myopathy is a feature of many inflammatory syndromes. Chronic inflammation has been linked to pathophysiological mechanisms which implicate 1,25 dihydroxyvitamin D3 (1,25-(OH)2D3)-mediated signaling pathways with emerging evidence supporting role for the vitamin D receptor (VDR) in contractile and metabolic function both skeletal cardiac muscle. Altered VDR expression muscle reported result significant effects on metabolism, calcium fibrosis these tissues. Elevated levels serum cytokines,...
// Angie M. Y. Shum 1, 2, * , Anne Poljak 3, 4, 5, Nicholas L. Bentley 3 Nigel Turner Timothy C. Tan 6 and Patsie Polly 2 1 Mechanisms of Disease Translational Research, School Medical Sciences, Faculty Medicine, UNSW Sydney, New South Wales, Australia Department Pathology, Pharmacology, 4 Bioanalytical Mass Spectrometry Facility, 5 Centre for Healthy Brain Ageing, Psychiatry, Western Clinical Westmead Hospital, Westmead, These authors contributed equally to this work Correspondence to:...
How did the onset of COVID-19 pandemic impact student learning in higher education? Everywhere, Sars-CoV-2 struck hardest most disadvantaged communities. This paper asks whether virus's disproportionate effect on more vulnerable groups is replicated among college and university students. Data come from approximately 3800 students studying at nine education institutions located six different countries around globe. Conventional imagery ‘Ivory Tower’ treats colleges universities as cloistered...
The vitamin D3 receptor (VDR), which is the nuclear for 1α,25-dihydroxyvitamin [1α,25(OH)2D3], acts primarily as a heterodimer with retinoid X (RXR) and binds preferentially to directly repeated arrangements of two hexameric binding sites three spacing nucleotides [DR3-type D response elements (VDREs)]. In this study, all presently known natural DR3-type VDREs have been compared classified on basis their complex-formation VDR–RXR heterodimers ability stabilize conformations. Based affinity...
Providing large numbers of undergraduate students in scientific disciplines with engaging, authentic laboratory experiences is important, but challenging. Virtual laboratories (vLABs) are a potential means to enable interactive learning experiences. A vLAB focusing on Western Blotting was developed and implemented 3rd year Pathology course for science facilitate technical molecular skills that linked development diagnostic skills. Such important undergraduates building conceptual...
The vitamin D receptor (VDR) is the nuclear for 1, 25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)] that acts as a ligand-dependent transcription factor via combined contact with coactivator proteins (steroid coactivator-1, transcriptional intermediary 2, and associated 3) specific DNA binding sites [vitamin response elements (VDREs)]. Ligand-mediated conformational changes of VDR contribute to key mechanisms in this hormone signaling process. 1alpha,25(OH)(2)D(3), MC1288...
Abstract Background: X-linked Charcot-Marie-Tooth type 1 disease has been associated with 280 mutations in the GJB1 [gap junction protein, beta 1, 32kDa (connexin 32, neuropathy, X-linked)] gene. High-resolution melting analysis an automated instrument can be used to scan DNA for alterations, but its use disorders not described. Methods: A 96-well LightScanner high resolution was amplicons of All reported this study had confirmed previously by sequence analysis. samples were amplified...
Cancer cachexia is a systemic, paraneoplastic syndrome seen in patients with advanced cancer. There growing interest the altered muscle pathophysiology experienced by cachectic patients. This study reports microarray analysis of gene expression cardiac and skeletal colon 26 (C26) carcinoma mouse model cancer cachexia. A total 268 genes were found to be differentially expressed tissue, compared nontumor-bearing controls. was fewer than 1,533 that changed muscle. In addition different numbers...
TERT gene rearrangement with transcriptional superenhancers leads to overexpression and neuroblastoma. No targeted therapy is available for clinical trials in patients TERT-rearranged neuroblastoma.Anticancer agents exerting the best synergistic anticancer effects BET bromodomain inhibitors were identified by screening an FDA-approved oncology drug library. The of inhibitor OTX015 proteasome carfilzomib examined immunoblot flow cytometry analysis. efficacy combination was investigated mice...
Fibronectin (FN) is an important adhesive noncollagenous glycoprotein involved in maintenance of the extracellular matrix and cell adhesiveness, loss which has been implicated metastatic potential cells. Regulation FN occurs at transcriptional level by active metabolite vitamin D3, 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). Transient transfection homologous heterologous promoter reporter constructs into ROS 17/2,8 (rat osteosarcoma), NIH 3T3 (mouse fibroblast), MCF-7 (human mammary carcinoma)...
The novel transcription factor hMusTRD1α1 (human muscle TFII-I repeat domain-containing protein 1α1; previously named MusTRD1; O'Mahoney, J. V., Guven, K. L., Lin, J., Joya, E., Robinson, C. S., Wade, R. P., and Hardeman, E. (1998) Mol. Cell. Biol. 18, 6641–6652) was identified in a yeast one-hybrid screen as that binds within an upstream enhancer-containing region of the skeletal muscle-specific gene, TNNI1 troponin I slow; hTnIslow). It has been proposed may play important role...
RUNX2 is an essential transcription factor required for skeletal development and cartilage formation. Haploinsufficiency of leads to cleidocranial displaysia (CCD) a disorder characterised by gross dysgenesis bones particularly those derived from intramembranous bone A notable feature the protein polyglutamine polyalanine (23Q/17A) domain coded repeat sequence. Since none known mutations causing CCD date map in glutamine region, we hypothesised that Q-repeat may be related more subtle...