A5074589045- Nitric Oxide and Endothelin Effects
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Tryptophan and brain disorders
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Schizophrenia research and treatment
- Blood disorders and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroscience and Neuropharmacology Research
- Electron Spin Resonance Studies
- Eicosanoids and Hypertension Pharmacology
- Air Quality and Health Impacts
- Diabetes Treatment and Management
- Pharmacological Effects and Toxicity Studies
- Diet, Metabolism, and Disease
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
- Phosphodiesterase function and regulation
- Liver Disease Diagnosis and Treatment
- Immune Response and Inflammation
- Health, Environment, Cognitive Aging
- Bone Metabolism and Diseases
- Neutropenia and Cancer Infections
Heinrich Heine University Düsseldorf
2011-2024
Bayer (Germany)
1994
Deutsches Diabetes-Zentrum e.V.
1989
Nitric oxide (NO) produced at high concentrations by the inducible NO synthase is an important effector molecule involved in immune regulation and defense. We have examined whether represents a signal for triggering apoptosis thymocytes. Freshly isolated thymocytes were incubated with different chemical donors various intervals. Apoptosis was determined detection of DNA strand breaks situ nick translation. All induced thymocyte 30% positive vs 10% controls after 8 h. prevented addition...
Activation of the nuclear enzyme poly(ADP-ribose) polymerase (PARP) is an early response cells exposed to DNA-damaging compounds such as nitric oxide (NO) or reactive oxygen intermediates (ROI). Excessive poly-(ADP-ribose) formation by PARP has been assumed deplete cellular NAD+ pools and induce death several cell types, including loss insulin-producing islet in type I diabetes. In present study we used from mice with a disrupted thus inactivated gene provide direct evidence for causal...
Article Nitric Oxide Generation during Cellular Metabolization of the Diabetogenic N-Mefhyl-N-Nitroso-Urea Streptozotozin Contributes to Islet Cell DNA Damage was published on January 1, 1995 in journal Biological Chemistry (volume 376, issue 3).
Abstract Objective To examine whether apoptosis contributes to the pathogenesis of skin lesions in patients with cutaneous lupus erythematosus (CLE) after ultraviolet (UV) irradiation. Methods In situ nick translation and TUNEL were performed detect 85 biopsy specimens from various subtypes CLE. Specimens normal healthy donors polymorphous light eruption used as controls. addition assessment primary lesions, provocative phototesting was carried out investigate events occurring secondary UV...
Psoriasis is a common chronic skin disease mediated by cellular immune mechanisms and characterized an intense neutrophil cell infiltrate proliferative activation of epidermal keratinocytes. We have previously described the expression inducible nitric oxide synthase (iNOS) in keratinocytes psoriatic lesions. In this study, role iNOS inflammation was explored ex vivo biopsies vitro primary cultures human Messenger RNA for enzyme (iNOS mRNA) detected reverse transcriptase polymerase chain...
NO has been identified recently as the prime islet-toxic product of inflammatory macrophages. The adverse effects IL-1 on isolated islets also have reported to involve NO. We now show that exposure an islet cell suspension donor nitroprusside or activated macrophages leads DNA strand breaks. Macrophages did not induce damage in presence synthase inhibitor NG-methyl-L-arginine. breaks were demonstrated at level single cells by a modified nick-translation procedure and confirmed analysis...
Abstract We investigated the effects of murine rTNF-alpha, human rIL-1 beta, and rat rIFN-gamma in various concentrations and/or combinations on inducible nitric oxide (NO) production primary cultures aortic endothelial cells. Northern blot analysis total RNA from induced control using cloned mouse macrophage gene NO synthase as probe well polymerase chain reaction a specific primer sequence gave positive signal for activated cells only. A approximately 4.4 kb length similar to form...
The present study examined cellular effects of the atypical antipsychotic drug clozapine on blood cells treated patients with and without clozapine-induced agranulocytosis (CA). Blood from one patient who commenced treatment was at weekly intervals for 128 days. Olanzapine-treated (n = 5) polymedicated 14) schizophrenic patients, as well healthy subjects 19) septic shock 8), were studied comparison. We observed dramatically increased numbers native neutrophils stained superoxide anion...
Background: The superior therapeutic benefit of clozapine is often associated with metabolic disruptions as obesity, insulin resistance, tachycardia, higher blood pressure, and even hypertension. Aims: These adverse vascular/ events under are similar to those caused by polycyclic aromatic hydrocarbons (PAHs), shows structural similarity well-known ligands the aryl hydrocarbon receptor (AhR). Therefore, we speculated that side effects might rely on AhR signaling. Methods: We examined...