A5068325499- Epigenetics and DNA Methylation
- Medical Imaging Techniques and Applications
- Medical and Biological Sciences
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Peroxisome Proliferator-Activated Receptors
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Genetic Syndromes and Imprinting
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Tryptophan and brain disorders
- Metabolism and Genetic Disorders
- Bone Metabolism and Diseases
- Bipolar Disorder and Treatment
- Adipose Tissue and Metabolism
- Sex and Gender in Healthcare
- Luminescence Properties of Advanced Materials
- 3D Printing in Biomedical Research
- Electronic and Structural Properties of Oxides
- Virus-based gene therapy research
Saitama Medical University
2013-2025
Kumamoto University
2019-2025
Zero to Three
2024
Murata (United States)
2023
Kubota (Japan)
2023
Juntendo University
2023
Genomics (United Kingdom)
2016-2017
Hidaka Hospital
2015
Kyushu Institute of Technology
2006-2008
Advanced Engineering (Czechia)
2007
Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses....
Mitochondrial disorders have the highest incidence among congenital metabolic characterized by biochemical respiratory chain complex deficiencies. It occurs at a rate of 1 in 5,000 births, and has phenotypic genetic heterogeneity. Mutations about 1,500 nuclear encoded mitochondrial proteins may cause dysfunction energy production disorders. More than 250 genes that been reported to date. However exact diagnosis for patients still remained largely unknown. To reveal this heterogeneity, we...
Abstract Meiosis is a unique process that allows the generation of reproductive cells. It remains largely unknown how meiosis initiated in germ cells and why non-germline do not undergo meiosis. We previously demonstrated knockdown Max expression, gene encoding partner MYC family proteins, strongly activates expression cell-related genes ESCs. Here we find complete ablation ESCs results profound cytological changes reminiscent undergoing meiotic cell division. Furthermore, our analyses...
Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis or age-related osteopenia is caused by the unbalanced differentiation MSCs into osteoblasts. Several transcription factors are known to regulate balance between adipocyte and osteoblast differentiation. However, molecular mechanisms that have yet be elucidated. To identify candidate genes associated with osteoporosis, we performed genome-wide expression analyses differentiating osteoblasts adipocytes. Among were...
COVID-19, caused by SARS-CoV-2, has spread worldwide with dire consequences. To urgently investigate the pathogenicity of COVID-19 and develop vaccines therapeutics, animal models that are highly susceptible to SARS-CoV-2 infection needed. In present study, we established an model via intratracheal tract in CAG promoter–driven human angiotensin-converting enzyme 2–transgenic (CAG-hACE2) mice. The CAG-hACE2 mice showed several severe symptoms infection, definitive weight loss subsequent...
Bipolar disorder (BD) is a severe mental characterized by repeated mood swings. Although genetic factors are collectively associated with the etiology of BD, underlying molecular mechanisms, particularly how environmental affect brain, remain largely unknown. We performed promoter-wide DNA methylation analysis neuronal and nonneuronal nuclei in prefrontal cortex patients BD (N = 34) controls 35). found decreased at promoters both cell types patients. Gene Ontology (GO) differentially...
Intense green, orange or magenta luminescence was observed in rare-earth doped alkaline earth (Ca, Sr, and Ba) stannates, particularly strontium stannates with perovskite-related structures. Tb–Mg codoped SrSnO3 showed sharp emission lines corresponding to Tb ions as a green phosphor. Sm Sr3Sn2O7 exhibited pairs of an Eu–Ti Sr2SnO4 phosphor red luminescent Eu well broad blue band Ti-related complexes. It suggested that codoping and/or layered structures these phosphors are closely related...
Abstract Once disseminated tumor cells (DTCs) arrive at a metastatic organ, they remain there, latent, and become seeds of metastasis. However, the clonal composition DTCs in latent state remains unclear. Here, we applied high-resolution DNA barcode tracking to mouse model that recapitulated dormancy head neck squamous cell carcinoma (HNSCC). We found clones abundantly circulated peripheral blood dominated DTCs. Through analyses multiple barcoded lines, identified specific subclonal...
Recent success with the use of glucagon-like peptide-1 (GLP-1) receptor analogs and dipeptidyl peptidase-4 (DPP-4) inhibitors for treatment patients diabetes has highlighted role intestine as an endocrine organ. Gut-derived hormones, including GLP-1, glucose-dependent insulinotropic polypeptide (GIP), ghrelin, have important roles in control energy metabolism food intake, are associated metabolic syndrome. In this study, we isolated identified a new intestine-derived hormone, betagenin,...
Abstract Background Long interspersed nuclear element-1 (LINE1 or L1) is a major type of retrotransposons constituting about 20% mammalian genomes, some which still have retrotransposition ability. L1 the only transposon that can retrotranspose itself using its own proteins. Although strictly repressed by host-defense mechanism, it known somatic transiently activated in early neural development. We previously reported increased copy number brains patients as well animal models psychiatric...
Peroxisomes are subcellular organelles involved in lipid metabolic processes, including those of very-long-chain fatty acids and branched-chain acids, among others. Peroxisome matrix proteins synthesized the cytoplasm. Targeting signals (PTS or peroxisomal targeting signal) at C-terminus (PTS1) N-terminus (PTS2) mediate their import into organelle. In case PTS2-containing proteins, PTS2 signal is cleaved from protein when transported peroxisomes. The functional mechanism processing, however,...
This report identifies a novel gene encoding Fam57b (family with sequence similarity 57, member B) as peroxisome proliferator-activated receptor γ (PPARγ)-responsive transmembrane that is related to obesity. The was identified based on an integrated bioinformatics analysis of the following three expression profiling data sets: adipocyte differentiation mouse stromal cells (ST2 cells), adipose tissues from obesity mice, and siRNA-mediated knockdown Pparγ using ST2 cells. consists variants...
Identification of a gene set capable driving rapid and proper reprogramming to induced pluripotent stem cells (iPSCs) is an important issue. Here we show that the efficiency kinetics iPSC are dramatically improved by combined expression Jarid2 genes encoding its associated proteins. We demonstrate forced JARID2 promotes suppressing Arf, known barrier, N-terminal half sufficient for such promotion. Moreover, accelerated silencing retroviral Klf4 transgene demethylation Nanog promoter,...
Background and AimsHepatocellular carcinoma (HCC) mainly develops from chronic hepatitis. Metabolic dysfunction-associated steatohepatitis (MASH) has gradually become the main pathogenic factor for HCC due to rising incidence of obesity metabolic diseases. 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is enzyme that degrades prostaglandin 2 (PGE2), which known exacerbate inflammatory responses. However, role PGE2 accumulation caused by 15-PGDH downregulation in development MASH-HCC not...
Correlation between amino acid composition and nucleotide is examined. Class III POU transcription factors having higher third GC contents showed of alanine, glycine, proline residues encoded by GC-rich nucleotides, vice versa. This correlation was observed even among various types from vertebrates invertebrates regardless functional structural constraints inherent to each protein. Furthermore, reptile class sequences revealed no evolutionary directionality increasing the cold- warm-blooded...
The epitaxial films of the recently developed calcium and strontium stannates phosphors with perovskite or its related structure were fabricated by pulsed-lased deposition method. all highly transparent in visible region, showed intense luminescence several colors under ultraviolet excitation. Light scattering at substrates' surfaces varied appearance photoluminescence. In combination epitaxial-film-growth technologies for many functional materials, obtained results provide a promise future...
Abstract Objective Mitochondrial respiratory chain disorder ( MRCD ) is an intractable disease of infants with variable clinical symptoms. Our goal was to identify the causative mutations in patients. Methods The subjects were 90 children diagnosed by enzyme assay. We analyzed whole mitochondrial DNA (mt sequences. A cybrid study performed two Whole exome sequencing for one these patients whose mt variant confirmed as non‐pathogenic. Results sequences identified 29 variants (13 previously...