A5038212374- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Growth Hormone and Insulin-like Growth Factors
- Salivary Gland Disorders and Functions
- Peroxisome Proliferator-Activated Receptors
- Advanced biosensing and bioanalysis techniques
- Hormonal and reproductive studies
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Nuclear Receptors and Signaling
- Neuroblastoma Research and Treatments
- Inflammatory mediators and NSAID effects
- Protein Degradation and Inhibitors
- interferon and immune responses
- Protein purification and stability
Saitama Medical University
2013-2024
Saitama Prefecture
2010-2015
Teikyo University of Science
2009
Tokushima Bunri University
2008
University of California, San Diego
1993-2003
Howard Hughes Medical Institute
1998-2000
Cellular Research (United States)
1999
Research Triangle Park Foundation
1999
Scripps Whittier Diabetes Institute
1998
La Jolla Institute For Molecular Medicine
1998
Different classes of mammalian transcription factors—nuclear receptors, cyclic adenosine 3′,5′-monophosphate–regulated enhancer binding protein (CREB), and signal transducer activator transcription-1 (STAT-1)—functionally require distinct components the coactivator complex, including CREB-binding (CBP/p300), nuclear receptor coactivators (NCoAs), p300/CBP-associated factor (p/CAF), based on their platform or assembly properties. Retinoic acid receptor, CREB, STAT-1 also different histone...
Valentina Perissi, Lena M. Staszewski, Eileen McInerney, Riki Kurokawa, Anna Krones, David W. Rose, Mill H. Lambert, Michael V. Milburn, Christopher K. Glass, and G. Rosenfeld University of California, San Diego (UCSD), Graduate Student, Molecular Pathology Program, Howard Hughes Medical Institute (HHMI), Department School Medicine, Cellular Division Endocrinology Metabolism, UCSD, La Jolla, California 92095-0648 USA; Structural Chemistry, Glaxco Wellcome Inc., Research Triangle Park, North...
Nuclear factor-kappaB (NF-kappaB) plays a role in the transcriptional regulation of genes involved inflammation and cell survival. In this report we demonstrate that NF-kappaB recruits coactivator complex has striking similarities to recruited by nuclear receptors. Inactivation either cyclic AMP response element binding protein (CREB)-binding (CBP), members p160 family coactivators, or CBP-associated factor (p/CAF) antibody microinjection prevents NF-kappaB-dependent transactivation. Like...
The estrogen receptor (ER) is a ligand-dependent transcription factor that regulates expression of target genes in response to concert with other cellular signaling pathways. This suggests the mechanism by which ER transmits an activating signal general machinery may include factors integrate these diverse signals. We have previously characterized receptor-associated protein, ERAP160, as complexes agonist-dependent manner. now found transcriptional coactivator p300 associates agonist bound...
Retinoic acid, thyroid hormone, and vitamin D receptors preferentially activate target genes through response elements that consist of direct repeat arrangements a core recognition motif consensus sequence AGGTCA. We present evidence the preference for arises from two fundamental differences steroid hormone receptors. First, retinoic are demonstrated to form heterodimers with retinoid X These interactions mediated by carboxy-terminal dimerization interface, heterodimer specified actions...
AbstractAs the obligate member of most nuclear receptor heterodimers, retinoid X receptors (RXRs) can potentially perform two functions: cooperative binding to hormone response elements and coordinate regulation target genes by RXR ligands. In this paper we describe allosteric interactions between heterodimeric partners, retinoic acid (RARs) peroxisome proliferator-activated (PPARs); RARs PPARs prevent permit activation RXR-specific ligands, respectively. By competing for dimerization with...
CREB binding protein (CBP) functions as an essential coactivator of transcription factors that are inhibited by the adenovirus early gene product E1A. Transcriptional activation signal transducer and activator transcription-1 (STAT1) requires C/H3 domain in CBP, which is primary target E1A inhibition. Here it was found not required for retinoic acid receptor (RAR) function, nor involved Instead, inhibits RAR function preventing assembly CBP-nuclear complexes, revealing differences CBP...
Members of the nuclear receptor superfamily are thought to activate transcription by recruitment one or more recently identified coactivator complexes. Here we demonstrate that both peroxisome proliferator-activated binding protein (PBP) and steroid coactivator-1 (SRC-1) required for ligand-dependent transiently transfected chromosomally integrated reporter genes estrogen (ER) retinoic acid (RAR). To examine interactions between receptors specific coactivators in living cells, these proteins...
The Ewing's sarcoma (EWS) oncogene contains an N-terminal transcription activation domain and a C-terminal RNA-binding domain. Although the EWS is potent transactivation that required for oncogenic activity of several fusion proteins, normal role intact poorly characterized because little known about its nucleic acid recognition specificity. Here we show Arg-Gly-Gly (RGG) in binds to G-rich single-stranded DNA RNA fold G-quadruplex structure. Furthermore, inhibition polymerase on template...
Synovial fibroblasts (SFs) produce matrix-degrading enzymes that cause joint destruction in rheumatoid arthritis (RA). Epigenetic mechanisms play a pivotal role autoimmune diseases. This study was undertaken to elucidate the epigenetic mechanism regulates transcription of matrix metalloproteinases (MMPs) RASFs.MMP gene expression and histone methylation profiles MMP promoters were examined RASFs. The effect WD repeat domain 5 (WDR5) silencing on RASFs analyzed. expression, surface...
Thyroid hormone (3,5,3'-triiodothyronine) positively regulates transcription of the sarcoplasmic reticulum Ca2+ATPase gene in rat heart, and sequences within 559 nucleotides upstream from start site confer thyroid responsiveness upon a reporter gene. In present study, three hormone-response elements (TREs) are identified between -485 -190. Each TRE is active transient transfection assays specifically binds 3,5,3'-triiodothyronine receptors (TRs) alpha 1 beta alone combination with retinoid X...
CREB-binding proteins (CBP) and p300 are essential transcriptional coactivators for a large number of regulated DNA-binding transcription factors, including CREB, nuclear receptors, STATs. CBP function in part by mediating the assembly multiprotein complexes that contain additional cofactors such as p300/CBP interacting protein (p/CIP), member p160/SRC family coactivators, associated factor p/CAF. In addition to serving molecular scaffolds, each possess intrinsic acetyltransferase activities...