A5017494539- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Apelin-related biomedical research
- Cardiac Fibrosis and Remodeling
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Long-Term Effects of COVID-19
- RNA modifications and cancer
- Renin-Angiotensin System Studies
- Galectins and Cancer Biology
- Protease and Inhibitor Mechanisms
- Immune cells in cancer
- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- Fuel Cells and Related Materials
- Cancer, Stress, Anesthesia, and Immune Response
- Nuclear Receptors and Signaling
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Aquaculture disease management and microbiota
Akita University
2017-2024
Kyushu University
2023-2024
Hiroshima General Hospital
2024
Kumamoto University
2007-2023
Elabela/Toddler/Apela (ELA) has been identified as a novel endogenous peptide ligand for APJ/Apelin receptor/Aplnr. ELA plays crucial role in early cardiac development of zebrafish well maintenance self-renewal human embryonic stem cells. Apelin was the first APJ ligand, and exerts positive inotropic heart effects regulates renin-angiotensin system. The aim this study to investigate biological cardiovascular system.Continuous infusion significantly suppressed pressure overload-induced...
Angiotensin-converting enzyme 2 (ACE2) is critically involved in cardiovascular physiology and pathology, currently clinically evaluated to treat acute lung failure. Here we show that the B38-CAP, a carboxypeptidase derived from Paenibacillus sp. B38, an ACE2-like decrease angiotensin II levels mice. In protein 3D structure analysis, B38-CAP homolog shares structural similarity mammalian ACE2 with low sequence identity. vitro, recombinant catalyzed conversion of 1-7, as well other known...
Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as decoy. carboxypeptidase that degrades angiotensin II, thereby improving the pathologies cardiovascular disease or acute lung injury. Here we show B38-CAP, an ACE2-like enzyme, protective against SARS-CoV-2-induced Endogenous expression downregulated in lungs SARS-CoV-2-infected hamsters, leading to elevation II levels. Recombinant Spike also...
Destabilization of Atg7 mRNA by the CCR4-NOT complex prevents p53-dependent cell death in heart.
Abstract Esophageal squamous cell carcinoma (ESCC) is one of the most fatal types malignant tumors worldwide. Epitranscriptome, such as N 6 ‐methyladenosine (m A) mRNA, an abundant post‐transcriptional mRNA modification and has been recently implicated to play roles in several cancers, whereas significance m A modifications virtually unknown ESCC. Analysis tissue microarray 177 ESCC patients showed that higher expression demethylase ALKBH5 correlated with poor prognosis was independent...
COVID-19, caused by SARS-CoV-2, has spread worldwide with dire consequences. To urgently investigate the pathogenicity of COVID-19 and develop vaccines therapeutics, animal models that are highly susceptible to SARS-CoV-2 infection needed. In present study, we established an model via intratracheal tract in CAG promoter–driven human angiotensin-converting enzyme 2–transgenic (CAG-hACE2) mice. The CAG-hACE2 mice showed several severe symptoms infection, definitive weight loss subsequent...
Shortening of mRNA poly(A) tails (deadenylation) to trigger their decay is mediated mainly by the CCR4-NOT deadenylase complex. While four catalytic subunits (CNOT6, 6L 7, and 8) have been identified in mammalian complex, individual biological roles are not fully understood. In this study, we addressed contribution CNOT7/8 viability primary mouse embryonic fibroblasts (MEFs). We found that MEFs lacking expression [Cnot7/8-double knockout (dKO) MEFs] undergo cell death, whereas CNOT6/6L...
The biological significance of deadenylation in global gene expression is not fully understood. Here, we show that the CCR4–NOT deadenylase complex maintains mRNAs, such as those encoding transcription factors, cell cycle regulators, DNA damage response–related proteins, and metabolic enzymes, at appropriate levels liver. Liver-specific disruption Cnot1 , a scaffold subunit complex, leads to increased mRNAs for proteins because reduced stabilization these mRNAs. CNOT1 suppression also...
Angiotensin-converting enzyme 2 (ACE2) is the carboxypeptidase to degrade angiotensin II (Ang II) 1–7 1–7) and improves pathologies of cardiovascular disease acute respiratory distress syndrome (ARDS)/acute lung injury. B38-CAP a bacteria-derived ACE2-like as potent human ACE2 ameliorates hypertension, heart failure SARS-CoV-2-induced injury in mice. Recombinant prepared with E . coli protein expression system more efficiently than recombinant soluble ACE2. Here we show therapeutic effects...
Localization of cellular oncogenes (c-onc) near the break points translocations in tumor cells has indicated involvement these genes neoplastic growth. Enhanced transcription homolog (c-abl) transforming sequence Abelson murine leukemia virus was observed K3D, which one cloned cell lines 7,12-dimethylbenz[a]anthracene-induced rat erythroblastic leukemia. Since c-abl activation not parent line (K2D) from K3D derived and latter different former presence a new marker chromosome, t(3;12), this...
Shortening of poly(A) tails triggers mRNA degradation; hence, deadenylation regulates many biological events. In the present study, we generated mice lacking Cnot1 gene, which encodes an essential scaffold subunit CCR4-NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and examined role adipocyte function. Cnot1-AKO showed reduced masses white tissue (WAT) brown (BAT), indicating abnormal organization function those tissues. Indeed, hyperinsulinemia, hyperglycemia, insulin...
Three chemical forms of atmospheric tritium, i. e., tritiated water vapor (HTO), hydrogen gas (HT), and hydrocarbons (CH3T), were measured at Kumamoto, Japan during the period between 2003 2005. The average concentrations in order HT, CH3T, HTO. yearly HT not so different each year, while that CH3T showed a large variation. all species have decreased compared to those 1984 1995 Fukuoka, about 100 km north present sampling location, suggesting decrease emissions from their sources for these...
Abstract Tumor‐derived G‐CSF is a well‐known factor to aggravate disease progression in various types of cancers. In this study, we investigated role squamous cell carcinoma (SCC). High expression the tumor tissues esophageal SCC (ESCC) patients correlated with poor prognosis. Murine NR‐S1M cells produce considerable amount G‐CSF, which its metastatic potentials. Deletion mitigated growth and metastasis lymph node lung subcutaneous tumors mice. Mechanistically, enhanced proliferation...
Acute lung injury (ALI) is caused by a variety of illnesses, including aspiration pneumonia and sepsis. The CCR4-NOT complex large multimeric protein that degrades mRNA through poly(A) tail shortening, whereas it also contributes to regulation transcription translation. Cnot3 scaffold component the essential for integrity complex; loss leads depletion whole complex. While significance cytokine degradation in limiting inflammation has been established, roles complex-mediated ALI remain elusive.
Heart failure is rapidly increasing and a growing burden on human health the economy in world. The functional role of mRNA regulation pathogenesis heart remains to be elucidated. Carbon catabolite repression 4-negative TATA-less complex multisubunit protein that deadenylates mRNA, process exonuclease-mediated degradation poly(A) tail. Here we show cardiac protective roles deadenylase subunit CNOT6L against stress. After 2 weeks transverse aortic constriction (TAC)-induced pressure overload,...
Abstract Pharmacological interventions to enhance fibrinolysis are effective for treating thrombotic disorders. Utilizing the in vitro U937 cell line-based fibrin degradation assay, we had previously found a cyclic pentapeptide malformin A 1 (MA ) as novel activating compound cellular fibrinolytic activity. The mechanism by which MA enhances activity remains unknown. In present study, show that RSK1 is crucial mediator of -induced fibrinolysis. Treatment with rhodamine-conjugated showed...
Abstract Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as decoy. the carboxypeptidase to degrade angiotensin II (Ang II) 1-7 improves pathologies cardiovascular disease acute lung injury. To address whether enzymatic activity protective against COVID-19, we investigated effects B38-CAP, an ACE2-like enzyme, on SARS-CoV-2-induced Expression was significantly downregulated in lungs...
The mechanism underlying cancer cell metastasis from the tumor to regional lymph nodes is not yet fully understood. We hypothesized that peritumoral neutrophil accumulation promotes node in thoracic esophageal squamous cancer.Between 2010 and 2019, 126 patients received curative (R0) esophagectomy without preoperative treatment our hospital. Using paraffin-embedded resected tumors, we performed immunohistochemical analysis of CD16b-positive area, which was defined as a 1-mm region centered...
Residual dissection is a concern in endovascular treatment with DCB, and there limited knowledge of hemodynamics at lesion. Therefore, the objective this study to evaluate mean pressure gradient (MPG) fractional flow reserve (FFR) residual after DCB angioplasty for superficial femoral artery (SFA).
Abstract Adipocyte differentiation is crucial for formation and expansion of white adipose tissue also associated with the pathologies obesity. CNOT4 an E3 ubiquitin ligase contains RNA binding domain. In mammals has been suggested to interact CCR4-NOT complex, a major executor mRNA poly(A) shortening. While several subunits within complex were shown be involved in obesity energy metabolism, roles remain unexplored. this study, we generated analyzed Cnot4 knockout mice found that...