A5002873416- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Invertebrate Immune Response Mechanisms
- Developmental Biology and Gene Regulation
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- Animal Behavior and Reproduction
- Advanced biosensing and bioanalysis techniques
- Hippo pathway signaling and YAP/TAZ
- Mitochondrial Function and Pathology
- Insect Resistance and Genetics
- Signaling Pathways in Disease
- Vitamin C and Antioxidants Research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Silk-based biomaterials and applications
- Monoclonal and Polyclonal Antibodies Research
- Heat shock proteins research
- Axon Guidance and Neuronal Signaling
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Metabolism and Genetic Disorders
Tokyo Metropolitan University
2015-2025
RIKEN
2018
RIKEN Advanced Science Institute
2012-2018
Tokyo Metropolitan Institute of Gerontology
1984-2011
The University of Tokyo
2008
Tohoku University
2002-2005
University of Basel
2005
Japan Science and Technology Agency
1999-2001
Heidelberg University
1994
University of Zurich
1988
Despite its morphological similarity to the other species in Drosophila melanogaster complex, D. sechellia has evolved distinct physiological and behavioral adaptations host plant Morinda citrifolia, commonly known as Tahitian Noni. The odor of ripe fruit M. citrifolia originates from hexanoic octanoic acid. is attracted these two fatty acids, whereas complex are repelled. Here, using interspecies hybrids between deficiency mutants sechellia, we showed that Odorant-binding protein 57e...
Mitochondrial disorders have the highest incidence among congenital metabolic characterized by biochemical respiratory chain complex deficiencies. It occurs at a rate of 1 in 5,000 births, and has phenotypic genetic heterogeneity. Mutations about 1,500 nuclear encoded mitochondrial proteins may cause dysfunction energy production disorders. More than 250 genes that been reported to date. However exact diagnosis for patients still remained largely unknown. To reveal this heterogeneity, we...
In Drosophila , microbial infection activates an antimicrobial defense system involving the activation of proteolytic cascades in hemolymph and intracellular signaling pathways, immune deficiency (imd) Toll immune-responsive tissues. The mechanisms for recognition are largely unknown. We report that, larvae, imd-mediated antibacterial is activated by peptidoglycan-recognition protein (PGRP)-LE, a PGRP-family member . Consistent with this, PGRP-LE binds to diaminopimelic acid-type...
Abstract We have constructed a P-element-based gene search vector for efficient detection of genes in Drosophila melanogaster. The contains two copies the upstream activating sequence (UAS) enhancer adjacent to core promoter, one copy near terminal inverted repeats at each end vector, and oriented direct transcription outward. Genes were detected on basis phenotypic changes caused by GAL4-dependent forced expression vector-flanking DNA, transcripts identified with reverse transcriptase PCR...
Decapentaplegic (Dpp), a Drosophila TGF beta/bone morphogenetic protein homolog, functions as morphogen to specify cell fate along the anteroposterior axis of wing. Dpp is heparin-binding and signal transduction potentiated by Dally, cell-surface heparan sulfate proteoglycan, during assembly several adult tissues. However, molecular mechanism which gradient established maintained poorly understood. We show evidence that Dally regulates both cellular responses distribution in In developing...
Egg activation is the process by which a mature oocyte becomes capable of supporting embryo development. In vertebrates and echinoderms, induced fertilization. Molecules introduced into egg sperm trigger progressive release intracellular calcium stores in oocyte. Calcium wave(s) spread through induce completion meiosis, new macromolecular synthesis, modification vitelline envelope to prevent polyspermy. However, arthropod eggs activate without fertilization: insects examined, as they move...
We have shown previously that mutations in the apico-basal cell polarity regulators cooperate with oncogenic Ras (Ras(ACT)) to promote tumorigenesis Drosophila melanogaster and mammalian cells. To identify novel genes Ras(ACT) tumorigenesis, we carried out a genome-wide screen for when overexpressed throughout developing eye enhance Ras(ACT)-driven hyperplasia. Ras(ACT)-cooperating identified were Rac1 Rho1, RhoGEF2, pbl, rib, east, which encode morphology regulators. In clonal setting,...
Notch is a transmembrane receptor that mediates the cell-cell interactions necessary for many cell-fate decisions. Endocytic trafficking of plays important roles in activation and downregulation this receptor. A Drosophila O-FucT-1 homolog, encoded by O-fut1, catalyzes O-fucosylation Notch, modification essential signaling ligand binding. It was recently proposed O-fut1 acts as chaperon endoplasmic reticulum required to exit reticulum. Here, we report has additional functions endocytic...
The downregulation of E-cadherin by Src promotes epithelial to mesenchymal transition and tumorigenesis. However, a simple loss cell adhesion is not sufficient explain the diverse developmental roles metastatic behavior viral Src-transformed cells. Here, we studied functions endogenous activated forms Drosophila in context tracheal development, during which extensive remodeling adherens junctions takes place. We show that Src42A selectively epithelia undergoing morphogenesis. Src64B are...
L-Ascorbic acid (AsA) is a water-soluble antioxidant. We examined the effect of AsA deficiency on skeletal muscle using senescence marker protein-30 (SMP30)-knockout (KO) mice that are defective in biosynthesis, which makes this mouse model similar to humans, clarify function muscle. Eight-week-old female SMP30-KO were divided into following two groups: an AsA-sufficient group [AsA(+)] was administered 1.5 g/L and AsA-deficient [AsA(-)] tap (AsA-free) water. At 4 weeks, content gastrocnemius...
longitudinals lacking ( lola ) is a complex Drosophila gene encoding at least 20 protein isoforms,each bearing the same N-terminal constant region linked to different C-terminal variable region. Different isoforms specify aspects of axon growth and guidance. We show here that mRNAs are generated by alternative trans -splicing exons sequentially encoded DNA strand. Chromosomal pairing facilitates interallelic -splicing,allowing complementation between mutations in those exons. demonstrate one...