A5006372948- Neurobiology and Insect Physiology Research
- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
- Insect Utilization and Effects
- Cellular Mechanics and Interactions
- Invertebrate Immune Response Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- Insect symbiosis and bacterial influences
- Microtubule and mitosis dynamics
- Insect and Arachnid Ecology and Behavior
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Chemical and Physical Properties in Aqueous Solutions
- Asphalt Pavement Performance Evaluation
- Insect behavior and control techniques
- Physiological and biochemical adaptations
- Renal and Vascular Pathologies
- Ionic liquids properties and applications
- Cell Adhesion Molecules Research
- Renal Transplantation Outcomes and Treatments
- Cancer therapeutics and mechanisms
- RNA Research and Splicing
- Adsorption and Cooling Systems
- Advanced Battery Materials and Technologies
Kyoto University
2016-2025
Centre de Recherche en Économie et Statistique
2022-2023
Centre for Research in Engineering Surface Technology
2022-2023
Center for Responsible Travel
2022-2023
Japan Agency for Medical Research and Development
2019-2023
Living Systems (United States)
2022-2023
Japan Science and Technology Agency
2002-2016
Kyoto Bunkyo University
2010
Kyoto University Institute for Chemical Research
2010
Ibaraki University
2009
A P-element vector has been constructed and used to generate lines of flies with single autosomal insertions. The were analyzed in two ways: (1) the identification cis-acting patterning information within Drosophila genome, as revealed by a lacZ reporter gene P element, (2) isolation lethal mutations. We examined 3768 independent for expression embryos looked among these mutations affecting embryonic neurogenesis. This type screen appears be an effective way find new loci that may play role...
αE-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role poorly understood. We transfected an αE-catenin–deficient colon carcinoma line with series of αE-catenin mutant constructs. The results showed that the amino acid 326–509 domain this catenin was to organize TJs, and COOH-terminal not essential process. internal found bind vinculin. When NH2-terminal fragment, which by itself unable TJ, fused vinculin tail, chimeric molecule could induce...
Dynamic epithelial reorganization is essential for morphogenesis of various organs. In Drosophila embryos, example the Malpighian tubule generated by cellular rearrangement a preexisting epithelium and tracheal network formed outgrowth, branching, fusion vesicles. Here we report that previously identified locus shotgun (shg) encodes DE-cadherin, an cell-cell adhesion molecule classic cadherin type zygotic shg mutations rather specifically impair processes dynamic morphogenesis. mutants,...
Growth cone motility and morphology are based on actin-filament dynamics. Cofilin plays an essential role for the rapid turnover of actin filaments by severing depolymerizing them. The activity cofilin is repressed phosphorylation at Ser3 LIM kinase (LIMK, in which acronym three gene products Lin-11, Isl-1, Mec-3) reactivated dephosphorylation phosphatases, termed Slingshot (SSH). We investigated roles cofilin, LIMK, SSH growth neurite extension expressing fluorescence protein-labeled LIMK1,...
Cofilin mediates lamellipodium extension and polarized cell migration by stimulating actin filament dynamics at the leading edge of migrating cells. is inactivated phosphorylation Ser-3 reactivated cofilin-phosphatase Slingshot-1L (SSH1L). Little known signaling mechanisms cofilin activation how this spatially regulated. Here, we show that activity SSH1L increases approximately 10-fold association with filaments, which indicates assembly per se triggers local thereby stimulates...
Proper neuronal function depends on forming three primary subcellular compartments: axons, dendrites, and soma. Each compartment has a specialized (the axon to send information, dendrites receive the soma is where most cellular components are produced). In mammalian neurons, each distinctive molecular morphological features, as well smaller domains, such initial segment, that have more functions. How compartments established maintained not understood. Genetic studies in Drosophila provided...
The oviduct is an important organ in reproduction where fertilization occurs, and through which the fertilized eggs are carried to uterus mammals. This highly polarized, epithelium forms longitudinal folds along ovary-uterus axis, epithelial multicilia beat towards transport ovulated ova. Here, we analyzed postnatal development of mouse report that multilevel polarities regulated by a planar cell polarity (PCP) gene, Celsr1. In epithelium, Celsr1 concentrated specific cellular boundaries...
Adenosine 5′-triphosphate (ATP) is the major energy currency of all living organisms. Despite its important functions, spatiotemporal dynamics ATP levels inside multicellular organisms unclear. In this study, we modified genetically encoded Förster resonance transfer (FRET)-based biosensor ATeam to optimize affinity at low temperatures. This new biosensor, AT1.03NL, detected changes Drosophila S2 cells more sensitively than original did, 25 °C. By expressing AT1.03NL in melanogaster and...
During development, neurons achieve a stereotyped neuron type-specific morphology, which relies on dynamic support by microtubules (MTs). An important player is the augmin complex (hereafter augmin), binds to existing MT filaments and recruits γ-tubulin ring (γ-TuRC), form branched MTs. In cultured neurons, for neurite formation. However, little known about role of during formation in vivo. Here, we have revisited mammalian culture then turned towards class four Drosophila dendritic...