A5011128677- Skin and Cellular Biology Research
- Dermatology and Skin Diseases
- Nail Diseases and Treatments
- Fungal Biology and Applications
- Skin Protection and Aging
- Cell Adhesion Molecules Research
- Wound Healing and Treatments
- Advancements in Transdermal Drug Delivery
- Proteoglycans and glycosaminoglycans research
- Allergic Rhinitis and Sensitization
- Plant Pathogens and Fungal Diseases
- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Electrospun Nanofibers in Biomedical Applications
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Plant tissue culture and regeneration
- 3D Printing in Biomedical Research
- Silk-based biomaterials and applications
- Animal testing and alternatives
- HER2/EGFR in Cancer Research
University of Namur
2016-2025
Namur Research Institute for Life Sciences
2006-2024
Namur Institute of Structured Matter
2023
IMEC
2023
Centre de Biophysique Moléculaire
2015
MRC Laboratory of Molecular Biology
2007
Mayo Clinic
1995
The final goal of the present study was development a 3-D chitosan dressing that would shorten healing time skin wounds by stimulating migration, invasion, and proliferation relevant cutaneous resident cells. Three-dimensional nanofibrillar scaffolds produced electrospinning were compared with evaporated films freeze-dried sponges for their biological properties. structure strongly improved cell adhesion in vitro. When implanted mice, scaffold colonized mesenchymal cells blood vessels....
Polysaccharide‐based nanofibers with a multilayered structure are prepared by combining electrospinning (ESP) and layer‐by‐layer (LBL) deposition techniques. Charged firstly poly(ε‐caprolactone) (PCL) block‐copolymer bearing carboxylic acid functions. After deprotonation of the groups, polyelectrolyte polysaccharides, notably chitosan hyaluronic acid, is used to coat electrospun fibers. A achieved alternating positively charged negatively polyelectrolyte. The construction this followed Zeta...
Abstract Background Hypoxia is a common characteristic of solid tumors associated with reduced response to radio- and chemotherapy, therefore increasing the probability tumor recurrence. The aim this study was identify new mechanisms responsible for hypoxia-induced resistance in breast cancer cells. Methods MDA-MB-231 HepG2 cells were incubated presence taxol or etoposide respectively under normoxia hypoxia apoptosis analysed. A whole transcriptome analysis performed order genes whose...
Keratinocyte monolayers, cultured in immersed conditions, constitute a frequently used vitro model system to study keratinocytes behaviour response environmental assaults. However, monolayers lack the keratinocyte terminal differentiation and organization of epidermal tissue, which are observed vivo. Advancements techniques were reconstruct three-dimensional equivalents that mimic human epidermis terms layering, barrier function. Here, we update published method illustrate progressive...
ABSTRACT Background Trichophyton benhamiae is a common dermatophyte whose natural host the guinea pig and which causes highly inflammatory skin lesions in humans. The subtilisin 6 (SUB6) of this fungus belongs to family 12 SUB genes. Its encoding gene, overexpressed vivo but not vitro, has been considered potentially important virulence factor, its role pathogenesis remains be elucidated. Objectives aim study was assess T. SUB6 mouse infection model. Methods To contribution virulence,...
In order to study cellular metabolism of low density lipoproteins (LDL), ultracentrifugal methods have been used isolate the lipoproteins.The use vertical rotor ultracentrifugation very quickly produces small quantities diluted per gradient, as well volumes lipoproteindeficient serum.We present modifications this method in prepare routinely more concentrated LDL and a sufficient volume lipoprotein-deficient serum relatively short time with minimal cost handling.-Pournay, Y.,...
Atopic dermatitis (AD) is a Th2-type inflammatory disease characterized by an alteration of epidermal barrier following the release IL-4 and IL-13. These cytokines activate type II IL-4Rα/IL-13Rα1 receptors in keratinocyte. Whilst IL-2Rγ, that forms I receptor for IL-4, only expressed haematopoietic cells, recent studies suggest its induction keratinocytes, which questions about role. We studied expression IL-2Rγ keratinocytes role keratinocyte function barrier. was using both reconstructed...
Ophiobolin A, a sesterterpenoid produced by plant pathogenic fungi, was purified from the culture extract of Drechslera gigantea and tested for its growth-inhibitory activity in both mammalian cells. A induced cell death Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells at concentrations ≥10 µM, with TBY-2 showing typical features apoptosis-like death. At concentration 5 ophiobolin did not affect viability but prevented proliferation. When on eight cancer lines, <1 µM inhibited growth...
Abstract TMEM45A ( DERP7 , DNAPTP4 or FLJ10134 ) gene, belonging to the TMEM family encoding predicted transmembrane proteins, is highly expressed in epidermal keratinocytes. To investigate potential involvement of during differentiation and keratinization processes, its expression has been characterized normal human keratinocytes protein subcellular localization studied this cell type, both vitro vivo . upregulated with differentiation, either induced by cultured keratinocyte confluence...