Stefano Gustincich
A5010889569- RNA Research and Splicing
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Genetic Neurodegenerative Diseases
- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- RNA regulation and disease
- Medical Imaging Techniques and Applications
- Retinal Development and Disorders
- Medical and Biological Sciences
- Advanced biosensing and bioanalysis techniques
- Olfactory and Sensory Function Studies
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Genetics and Neurodevelopmental Disorders
Italian Institute of Technology
2016-2025
Scuola Internazionale Superiore di Studi Avanzati
2014-2023
Istituto Universitario di Studi Superiori di Pavia
2010-2023
AREA Science Park
2006-2023
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2023
University of Trento
2022
Institute of Informatics and Telematics
2021
Bellvitge University Hospital
2012
Institut d'Investigació Biomédica de Bellvitge
2012
Harvard University
1999-2011
This study describes comprehensive polling of transcription start and termination sites analysis previously unidentified full-length complementary DNAs derived from the mouse genome. We identify 5' 3' boundaries 181,047 transcripts with extensive variation in arising alternative promoter usage, splicing, polyadenylation. There are 16,247 new protein-coding transcripts, including 5154 encoding proteins. Genomic mapping transcriptome reveals transcriptional forests, overlapping on both...
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There an international collaborative effort to identify all full-length mRNA transcripts from mouse, and ensure that each represented in physical collection clones. Here we report manual annotation 60,770 complementary DNA sequences. These are clustered 33,409 'transcriptional units', contributing 90.1% newly established transcriptome database. Of these transcriptional units, 4,258 new...
Abstract In the FANTOM5 project, transcription initiation events across human and mouse genomes were mapped at a single base-pair resolution their frequencies monitored by CAGE (Cap Analysis of Gene Expression) coupled with single-molecule sequencing. Approximately three thousands samples, consisting variety primary cells, tissues, cell lines, time series samples during activation development, subjected to uniform pipeline data production. The analysis started measuring RNA extracts assess...
The mechanisms that determine distinct embryonic pallial identities remain elusive. central role of Wnt signaling in directing dorsal telencephalic progenitors to the isocortex or hippocampus has been elucidated. Here, we show timely inhibition MAPK/ERK and BMP neuralized mouse stem cells (ESCs) specifies a cell identity characteristic allocortex. Comparison global gene expression profiles neural generated by (MiBi cells) with those from early postnatal encephalic regions reveals MiBi cells,...
The mesencephalic dopaminergic (mDA) cell system is composed of two major groups projecting cells in the substantia nigra (SN) (A9 neurons) and ventral tegmental area (VTA) (A10 cells). A9 neurons form nigrostriatal pathway are involved regulating voluntary movements postural reflexes. Their selective degeneration leads to Parkinson's disease. Here, we report that gene expression analysis (DA) identifies transcripts for α- β-chains hemoglobin (Hb). Globin immunoreactivity decorates majority...
Finding and characterizing mRNAs, their transcription start sites (TSS), associated promoters is a major focus in post-genome biology. Mammalian cells have at least 5–10 magnitudes more TSS than previously believed, deeper sequencing necessary to detect all active given tissue. Here, we present new method for high-throughput of 5′ cDNA tags—DeepCAGE: merging the Cap Analysis Gene Expression with ultra-high-throughput sequence technology. We apply DeepCAGE characterize 1.4 million sequenced...
Parkinson disease (PD) is no longer considered a complex motor disorder but rather systemic with variable nonmotor deficits that may include impaired olfaction, depression, mood and sleep disorders, altered cortical function. Increasing evidence indicates multiple metabolic defects occur in regions outside the substantia nigra, including cerebral cortex, even at premotor stages of disease. We investigated changes gene expression frontal cortex PD patient brains using transcriptomics...
Naturally occurring regulatory T (Treg) cells, which specifically express the transcription factor forkhead box P3 (Foxp3), are engaged in maintenance of immunological self-tolerance and homeostasis. By transcriptional start site cluster analysis, we assessed here how genome-wide patterns DNA methylation or Foxp3 binding sites were associated with Treg-specific gene expression. We found that hypomethylated regions closely Treg up-regulated clusters, whereas had no significant correlation...
Antisense (AS) transcripts are RNA molecules that transcribed from the opposite strand to sense (S) genes forming S/AS pairs. The most prominent configuration is when a lncRNA antisense protein coding gene. Increasing evidences prove transcription may control gene expression acting at distinct regulatory levels. However, its contribution brain function and neurodegenerative diseases remains unclear. We have recently identified AS Uchl1 as an mouse Ubiquitin carboxy-terminal hydrolase L1...
In anamniote embryos, the major wave of zygotic genome activation starts during mid-blastula transition. However, some genes escape global repression, are activated substantially earlier, and contribute to minor activation. The mechanisms underlying little understood. We explored genomic organization cis-regulatory a transcription body, in which is first detected zebrafish. identified miR-430 cluster as having excessive copy number highest density Pol-II-transcribed promoters genome, this...
Long Interspersed Nuclear Elements-1s (L1s) are transposable elements that constitute most of the genome's transcriptional output yet have still largely unknown functions. Here we show L1s required for proper mouse brain corticogenesis operating as regulatory long non-coding RNAs. They contribute to regulation balance between neuronal progenitors and differentiation, migration post-mitotic neurons proportions different cell types. In cortical cultured neurons, L1 RNAs mainly associated...
We report the construction of mouse full-length cDNA encyclopedia,the most extensive view a complex transcriptome,on basis preparing and sequencing 246 libraries. Before cloning,cDNAs were enriched in by Cap-Trapper,and cases,aggressively subtracted/normalized. have produced 1,442,236 successful 3′-end sequences clustered into 171,144 groups, from which 60,770 clones fully sequenced cDNAs annotated FANTOM-2 annotation. also 547,149 5′ end reads,which 124,258 groups. Altogether, these further...