Yuka Imamura Kawasawa
A5012097110- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- RNA Research and Splicing
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Immune cells in cancer
- Birth, Development, and Health
- Neurogenesis and neuroplasticity mechanisms
- Bioactive natural compounds
- Immune Response and Inflammation
- Oral Health Pathology and Treatment
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Acute Lymphoblastic Leukemia research
- Extracellular vesicles in disease
- Neuroscience and Neuropharmacology Research
- Obesity, Physical Activity, Diet
- Biochemical Analysis and Sensing Techniques
- Prenatal Substance Exposure Effects
- Immune Cell Function and Interaction
- Synthesis of Organic Compounds
- Genomics and Chromatin Dynamics
- Acute Myeloid Leukemia Research
Pennsylvania State University
2016-2025
Wake Forest Baptist Comprehensive Cancer Center
2024
Penn State Milton S. Hershey Medical Center
2015-2023
Children's National
2022
Kyoto University Hospital
2022
Center for Personalized Cancer Treatment
1998-2021
Yale University
2008-2020
Dokkyo Medical University
2020
Mackay Memorial Hospital
2019
Mackay Medical College
2019
This study describes comprehensive polling of transcription start and termination sites analysis previously unidentified full-length complementary DNAs derived from the mouse genome. We identify 5' 3' boundaries 181,047 transcripts with extensive variation in arising alternative promoter usage, splicing, polyadenylation. There are 16,247 new protein-coding transcripts, including 5154 encoding proteins. Genomic mapping transcriptome reveals transcriptional forests, overlapping on both...
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There an international collaborative effort to identify all full-length mRNA transcripts from mouse, and ensure that each represented in physical collection clones. Here we report manual annotation 60,770 complementary DNA sequences. These are clustered 33,409 'transcriptional units', contributing 90.1% newly established transcriptome database. Of these transcriptional units, 4,258 new...
INTRODUCTION The brain is responsible for cognition, behavior, and much of what makes us uniquely human. development the a highly complex process, this process reliant on precise regulation molecular cellular events grounded in spatiotemporal transcriptome. Disruption can lead to neuropsychiatric disorders. RATIONALE regulatory, epigenomic, transcriptomic features human have not been comprehensively compiled across time, regions, or cell types. Understanding etiology disorders requires...
INTRODUCTION Improved understanding of how the developing human nervous system differs from that closely related nonhuman primates is fundamental for teasing out human-specific aspects behavior, cognition, and disorders. RATIONALE The shared unique functional properties are rooted in complex transcriptional programs governing development distinct cell types, neural circuits, regions. However, precise molecular mechanisms underlying features have been only minimally characterized. RESULTS We...
The makings of the primate brain Although nonhuman brains are similar to our own, disparity between their and cognitive abilities tells us that surface similarity is not whole story. Sousa et al. overlaid transcriptome histological analyses see what makes human different from those primates. Various differentially expressed genes, such as encoding transcription factors, could alter transcriptional programs. Others were associated with neuromodulatory systems. Furthermore, dopaminergic...
Neocortical projection neurons exhibit layer-specific molecular profiles and axonal connections. Here we show that the identities of early-born subplate deep-layer are not acquired solely during generation or shortly thereafter but undergo progressive postmitotic refinement mediated by SOX5. Fezf2 Bcl11b, transiently expressed in all subtypes newly postmigratory neurons, subsequently downregulated layer 6 thereby establishing their 5-enriched postnatal patterns. In Sox5-null mice, this...
Here, we report the isolation and characterization of an endogenous peptide ligand GPR103 from rat brains. The purified was found to be 43-residue RF-amide QRFP. We also describe two mouse homologues human GPR103, termed GPR103A GPR103B. QRFP binds activates as well GPR103B, with nanomolar affinities in transfected cells. Systematic situ hybridization analysis brains showed that is expressed exclusively periventricular lateral hypothalamus, whereas receptor mRNAs are distinctly localized...
Exposure to stress during early life (infancy/childhood) has long-term effects on the structure and function of prefrontal cortex (PFC), increases risk for adult depression anxiety disorders. However, little is known about molecular cellular mechanisms these effects. Here, we focused changes induced by chronic maternal separation first 2 weeks postnatal life. Unbiased mRNA expression profiling in medial PFC (mPFC) maternally separated (MS) pups identified an increased myelin-related genes a...
Abstract Discrete bladder cancer molecular subtypes exhibit differential clinical aggressiveness and therapeutic response, which may have significant implications for identifying novel treatments this common malignancy. However, research is hindered by the lack of suitable models to study each subtype. To address limitation, we classified cell lines into using publically available data in Cancer Cell Line Encyclopedia (CCLE), guided genomic characterization The Genome Atlas (TCGA). This...
The disappearance of fine motor control Manual skills are much better developed in primates than rodents. This difference is part due to species-specific differences the motoneurons by brain. Gu et al. used a range approaches evaluate potential corticospinal tract projections neonatal mice. These exist immediately after birth but disappear within first 2 postnatal weeks owing actions plexin A, member semaphorin receptor family. Targeted deletion receptors mutant mice prevented elimination...
The process of phagophore closure requires the endosomal sorting complex required for transport III (ESCRT-III) subunit CHMP2A and AAA ATPase VPS4, but their regulatory mechanisms remain unknown. Here, we establish a FACS-based HaloTag-LC3 autophagosome completion assay to screen genome-wide CRISPR library identify ESCRT-I VPS37A as critical component closure. localizes on through N-terminal putative ubiquitin E2 variant domain, which is found be dispensable formation degradation epidermal...
Gut-resident CX3CR1 hi macrophages drive formation of tertiary lymphoid structures that initiate IgA responses to Salmonella infection.
Hypoxic damage to the developing brain due preterm birth causes many anatomical changes, including periventricular white matter. This results in loss of glial cells, significant disruptions myelination, and thereby cognitive behavioral disabilities seen throughout life. Encouragingly, these neurological morbidities can be improved by environmental factors; however, underlying cellular mechanisms remain unknown. We found that early continuous enrichment selectively enhances endogenous repair...
Type 1 interferon (T1IFN) signaling promotes inflammation and lupus pathology, but its role in autoreactive B cell development the antibody-forming (AFC) germinal center (GC) pathways is unclear. Using a model that allows for focused study of AFC GC responses, we show T1IFN crucial pathways. Through bone marrow chimeras, DNA-reactive transfer, GC-specific Cre mice, confirm IFNαR cells into both Transcriptomic analysis reveals gene expression alterations multiple non-GC absence IFNαR....
Abstract YAP1 fusion-positive supratentorial ependymomas predominantly occur in infants, but the molecular mechanisms of oncogenesis are unknown. Here we show YAP1-MAMLD1 fusions sufficient to drive malignant transformation mice, and resulting tumors share histo-molecular characteristics human ependymomas. Nuclear localization protein is mediated by MAMLD1 independent YAP1-Ser127 phosphorylation. Chromatin immunoprecipitation-sequencing analyses YAP1-MAMLD1-positive ependymoma reveal...
Compromised placental function or premature loss has been linked to diverse neurodevelopmental disorders. Here we show that placenta allopregnanolone (ALLO), a progesterone-derived GABA-A receptor (GABAAR) modulator, reduction alters neurodevelopment in sex-linked manner. A new conditional mouse model, which the gene encoding ALLO's synthetic enzyme (akr1c14) is specifically deleted trophoblasts, directly demonstrated ALLO insufficiency led cerebellar white matter abnormalities correlated...
Cortical excitatory glutamatergic projection neurons and inhibitory GABAergic interneurons follow substantially different developmental programs. In rodents, originate from progenitors within the dorsal forebrain, whereas arise in ventral forebrain. contrast, it has been proposed that humans, majority of cortical suggesting their origin migration is complex evolutionarily divergent. However, whether molecularly defined human interneuron subtypes distinct progenitors, including those remains...
The calcium-permeable ion channel TRPM2 is highly expressed in a number of cancers. In neuroblastoma, full-length (TRPM2-L) protected cells from moderate oxidative stress through increased levels forkhead box transcription factor 3a (FOXO3a) and superoxide dismutase 2. Cells expressing the dominant negative short isoform (TRPM2-S) had reduced FOXO3a 2 levels, calcium influx response to stress, enhanced reactive oxygen species, leading decreased cell viability. Here, xenografts generated with...
Background While the morbidity and mortality from cancer are largely attributable to its metastatic dissemination, integral features of cascade not well understood. The widely accepted hypothesis is that primary tumor microenvironment induces epithelial-to-mesenchymal transition in cells, facilitating their escape into bloodstream, possibly accompanied by stem cells. An alternative theory for metastasis involves fusion macrophages with cells (MTFs). Here we culture characterize apparent MTFs...