A5028447404- Tendon Structure and Treatment
- Lipid Membrane Structure and Behavior
- Protease and Inhibitor Mechanisms
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Electrostatics and Colloid Interactions
- MicroRNA in disease regulation
- Polymer Surface Interaction Studies
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- Risk Perception and Management
- Sports injuries and prevention
- Neonatal Respiratory Health Research
- Proteoglycans and glycosaminoglycans research
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Surfactants and Colloidal Systems
- Force Microscopy Techniques and Applications
- Green IT and Sustainability
- Innovative Approaches in Technology and Social Development
- Crop Yield and Soil Fertility
- Immune cells in cancer
- Biofuel production and bioconversion
- Rice Cultivation and Yield Improvement
- Biochemical and Structural Characterization
Ambo University
2016-2025
University of Ibadan
2024
Pennsylvania State University
2014-2024
Manchester University NHS Foundation Trust
2024
Stroke Association
2021-2023
Penn State Milton S. Hershey Medical Center
2014-2022
George Washington University
2022
Rajagiri Hospital
2022
University of Hong Kong
2007-2021
Core Laboratories (United States)
2018-2021
Background— Intimal smooth muscle cells (SMCs) contribute to the foam cell population in arterial plaque, and express lower levels of cholesterol exporter ATP-binding cassette transporter A1 (ABCA1) comparison with medial SMCs. The relative contribution SMCs total their expression ABCA1 intimal monocyte-derived macrophages, however, are unknown. Although macrophage markers by following lipid loading has been described, relevance this phenotypic switch human coronary atherosclerosis not...
Abstract The mechanism of phagophore closure remains unclear due to technical limitations in distinguishing unclosed and closed autophagosomal membranes. Here, we report the HaloTag-LC3 autophagosome completion assay that specifically detects phagophores, nascent autophagosomes, mature autophagic structures. Using this assay, identify endosomal sorting complexes required for transport (ESCRT)-III component CHMP2A as a critical regulator closure. During autophagy, translocates regulates...
To study the levels of perceived threat, severity, vulnerability, response efficacy, and self-efficacy for severe acute respiratory syndrome (SARS) eight other diseases in five European three Asian countries.A computer-assisted phone survey was conducted among 3,436 respondents. The questionnaire focused on related to SARS diseases.Perceived threat case an outbreak country higher than that diseases. Perceived vulnerability at intermediate level severity high compared varied between countries...
Mathematical and computational models provide valuable tools that help public health planners to evaluate competing interventions, especially for novel circumstances cannot be examined through observational or controlled studies, such as pandemic influenza. The spread of diseases like influenza depends on the mixing patterns within population, these depend in part local factors including spatial distribution age structure size composition households, employment status commuting adults,...
Patients with ischemic stroke attributed to large- or small-vessel disease are not considered at high risk for atrial fibrillation (AF), and the AF incidence rate in this population is unknown.To determine whether long-term cardiac monitoring more effective than usual care detection patients through 12 months of follow-up.The STROKE-AF trial was a randomized (1:1), multicenter (33 sites US) clinical that enrolled 496 between April 2016 July 2019, primary end point follow-up August 2020....
During autumn 2005, we conducted 3,436 interviews in European and Asian countries. We found risk perceptions of avian influenza to be at an intermediate level beliefs efficacy slightly lower. Risk were higher Europe than Asia; [corrected] lower Asia.
Rationale : Myocardial infarction (MI) results in remodeling of the myocardium and extracellular matrix (ECM). Tissue inhibitors metalloproteinases (TIMPs) are critical regulators ECM integrity via inhibiting (MMPs). TIMP2 is highly expressed heart only TIMP that, addition to MMPs, required for cell surface activation pro-MMP2. Hence, it difficult predict function as protective (MMP-inhibiting) or harmful (MMP-activating) disease. Objective We examined role cardiac response MI. Methods...
Changes in cellular cholesterol affect insulin secretion, and β-cell-specific deletion or loss-of-function mutations the efflux transporter ATP-binding cassette A1 (ABCA1) result impaired glucose tolerance β-cell dysfunction. Upregulation of ABCA1 expression may therefore be beneficial for maintenance normal islet function diabetes. Studies suggest that microRNA-33a (miR-33a) inversely correlates with hepatocytes macrophages. We examined whether miR-33a regulates pancreatic islets, thereby...
The process of phagophore closure requires the endosomal sorting complex required for transport III (ESCRT-III) subunit CHMP2A and AAA ATPase VPS4, but their regulatory mechanisms remain unknown. Here, we establish a FACS-based HaloTag-LC3 autophagosome completion assay to screen genome-wide CRISPR library identify ESCRT-I VPS37A as critical component closure. localizes on through N-terminal putative ubiquitin E2 variant domain, which is found be dispensable formation degradation epidermal...
Pancreatic ductal adenocarcinoma (PDAC) tumor growth is enhanced by tumor-associated macrophages (TAMs), yet the mechanisms which cells and TAMs communicate are not fully understood. Here we show that exosomes secreted PDAC cell lines differed in their surface proteins, lipid composition, efficiency of fusing with THP-1-derived vitro. Exosomes from AsPC-1, an ascites-derived human line, were enriched ICAM-1, mediated docking to through interactions surface-exposed CD11c on macrophages....
Abstract FOXP3 is required for the development of Treg and its expression often used as a surrogate marker functional suppression. However, it now known that activated human T effector cells can also express without acquiring regulatory activity. To more closely examine requirements to reprogram into Treg, we developed conditionally active form show here full acquisition phenotype function strictly dependent on amount cell expresses. In addition, phenotypic alterations induced by are only...
The calcium-permeable ion channel TRPM2 is highly expressed in a number of cancers. In neuroblastoma, full-length (TRPM2-L) protected cells from moderate oxidative stress through increased levels forkhead box transcription factor 3a (FOXO3a) and superoxide dismutase 2. Cells expressing the dominant negative short isoform (TRPM2-S) had reduced FOXO3a 2 levels, calcium influx response to stress, enhanced reactive oxygen species, leading decreased cell viability. Here, xenografts generated with...
Background While the morbidity and mortality from cancer are largely attributable to its metastatic dissemination, integral features of cascade not well understood. The widely accepted hypothesis is that primary tumor microenvironment induces epithelial-to-mesenchymal transition in cells, facilitating their escape into bloodstream, possibly accompanied by stem cells. An alternative theory for metastasis involves fusion macrophages with cells (MTFs). Here we culture characterize apparent MTFs...
The influenza pandemic of 2009 revealed shortcomings in the existing guidelines for risk and outbreak communication. Concepts such as building trust proved hard to achieve practice, whereas other issues communicating through internet coping with political fallout disease outbreaks are not dealt guidelines. This article surveys current makes recommendations additional tools be developed four areas: integrating long-term behavior change models communications; research develop a better...
Here we describe isolation and characterization of macrophage-tumor cell fusions (MTFs) from the blood pancreatic ductal adenocarcinoma (PDAC) patients. The MTFs were generally aneuploidy, immunophenotypic characterizations showed that express markers characteristic PDAC stem cells, as well M2-polarized macrophages. Single RNASeq analyses many transcripts implicated in cancer progression, LINE1 retrotransposons, very high levels several long non-coding involved metastasis (such MALAT1). When...
Extracellular matrix (ECM) remodeling is a critical aspect of cardiac following myocardial infarction. Tissue inhibitors metalloproteinases (TIMPs) are physiological (MMPs) that degrade the ECM proteins. TIMP3 highly expressed in heart, and markedly downregulated patients with ischemic cardiomyopathy. We therefore examined time- region-dependent role response to infarction (MI). TIMP3(-/-) wild-type (WT) mice were subjected MI by ligation left anterior descending artery. TIMP3(-/-)-MI...
Granzyme B (GZMB) is a proapoptotic serine protease that released by cytotoxic lymphocytes. However, GZMB can also be produced other cell types and capable of cleaving extracellular matrix (ECM) proteins. contributes to abdominal aortic aneurysm (AAA) through an extracellular, perforin-independent mechanism involving ECM cleavage. The murine inhibitor, Serpina3n (SA3N), inhibitor GZMB. In the present study, administration SA3N was assessed using mouse Angiotensin II-induced AAA model. Mice...
Atg9 is a multispanning transmembrane protein that required for autophagosome formation. During autophagy, vesicles containing are generated through an unknown mechanism and delivered to the formation sites. We have previously reported Atg9-containing membranes undergo continuous tubulation fission during nutrient starvation in manner dependent on curvature-inducing Bif-1/Sh3glb1. Here, we identify Dynamin 2 (DNM2) as Bif-1-interacting mediates of autophagy. The interaction Bif-1 DNM2...