A5062270994- Biomarkers in Disease Mechanisms
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Neuroblastoma Research and Treatments
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- S100 Proteins and Annexins
- Cell death mechanisms and regulation
- Ferroptosis and cancer prognosis
- Immune Response and Inflammation
- Cellular transport and secretion
- Hematopoietic Stem Cell Transplantation
- Adrenal and Paraganglionic Tumors
- Lymphadenopathy Diagnosis and Analysis
- Acute Lymphoblastic Leukemia research
- Sarcoma Diagnosis and Treatment
- Connexins and lens biology
- NF-κB Signaling Pathways
- Effects of Radiation Exposure
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Mesenchymal stem cell research
University of Pennsylvania
2025
Pennsylvania State University
2016-2024
Penn State Milton S. Hershey Medical Center
2018-2024
Haverford College
2011
Abstract The mechanism of phagophore closure remains unclear due to technical limitations in distinguishing unclosed and closed autophagosomal membranes. Here, we report the HaloTag-LC3 autophagosome completion assay that specifically detects phagophores, nascent autophagosomes, mature autophagic structures. Using this assay, identify endosomal sorting complexes required for transport (ESCRT)-III component CHMP2A as a critical regulator closure. During autophagy, translocates regulates...
Non-invasive prognostic biomarkers to inform clinical decision-making are an urgent unmet need for the management of patients with glioblastoma (GBM). We previously showed that higher circulating cell-free DNA concentration [ccfDNA] is associated worse survival in GBM. However, biology underlying this unknown. prospectively enrolled 129 treatment-naïve GBM blood drawn prior initial resection (baseline) and at time first post-radiotherapy MRI. performed ccfDNA methylation deconvolution...
Abstract Neuroblastoma is the most common extracranial solid malignancy in pediatric population, accounting for over 9% of all cancer-related deaths children. Autophagy a cell self-protective mechanism that promotes tumor growth and survival, making it an attractive target treating cancer. However, role autophagy neuroblastoma metastasis largely undefined. Here we demonstrate targeted inhibition essential kinase, unc-51 like kinase 1 (ULK1), with recently developed small-molecule inhibitor...
Background Anti-GD2 monoclonal antibody immunotherapy has significantly improved the overall survival rate for high-risk neuroblastoma patients. However, 40% of patients fail to respond or develop resistance treatment, and molecular mechanisms by which this occurs remain poorly understood. Tumor-derived small extracellular vesicles (sEVs) have emerged as critical regulators in modulating response immunotherapy. In study, we investigated role neuroblastoma-derived sEVs promoting anti-GD2...
Abstract Although neoadjuvant chemotherapy is a standard component of breast cancer treatment, recent evidence suggests that chemotherapeutic drugs can promote metastasis through poorly defined mechanisms. Here we utilize xenograft mouse models triple-negative to explore the importance chemotherapy-induced tumor-derived small extracellular vesicles (sEV) in metastasis. Doxorubicin (DXR) enhanced tumor cell sEV secretion accelerate pulmonary by priming premetastatic niche. Proteomic analysis...
Abstract Background Novel treatments are needed for oligodendroglioma that has recurred following radiotherapy (RT) and chemotherapy. The cyclin D1–CDK4 axis is frequently dysregulated in oligodendroglioma. Abemaciclib a selective CDK4/6 inhibitor achieves pharmacologically relevant concentrations brain tumor tissue. Methods We conducted single-arm, phase 2 trial evaluating the efficacy of abemaciclib patients with recurrent oligodendroglioma, isocitrate dehydrogenase (IDH)-mutant...
<p>Supplemental Figure 1. Patients and assays Supplemental 2. Survival analysis at baseline post-radiation 3. Correlative of all ccfDNA deconvolution subsets with [ccfDNA]. 4. Analysis % neutrophil tissue DNA pre-operative steroids. 5. [citH3]. 6. 7. [citH3], steroid dose, tumor volume. 8. by exposure. 9. Percent [citH3] time first MRI. 10. total [ccfDNA] 11. Molecular sub-type Clusters 1 2 from plasma differential methylation results. 12. Unsupervised clustering data, methylation,...
<div>AbstractPurpose:<p>Noninvasive prognostic biomarkers to inform clinical decision-making are an urgent unmet need for the management of patients with glioblastoma (GBM). We previously showed that higher circulating cell-free DNA (ccfDNA) concentration is associated worse survival in GBM. However, biology underlying this unknown.</p>Experimental Design:<p>We prospectively enrolled 129 treatment-naïve GBM blood drawn prior initial resection (baseline) and at time...
Enlarged lymph nodes are a common complaint in Pediatrician's office. Diagnosis of reactive lymphadenopathy secondary to infectious, inflammatory, immune dysregulation calls for clinical investigation, including thorough history, physical exam, imaging, and less often, biopsy the node. Here we discuss rare presentation extensive generalized, chronic, waxing, waning diagnosed as Progressive Transformation Germinal Centers (PTGC) course illness over eight years follow up period.Progressive is...
Purpose: To investigate the role of protein misfolding in retinal pigment epithelial (RPE) cell dysfunction, effects R345W-Fibulin-3 expression on RPE phenotype were studied. Methods: Primary cells cultured to confluence Transwells and infected with lentivirus constructs express wild-type (WT)- or R345W-Fibulin-3. Barrier function was assessed by evaluating zonula occludens-1 (ZO-1) distribution trans-epithelial electrical resistance (TER). Polarized secretion vascular endothelial growth...