A5081651802- Retinal Diseases and Treatments
- Extracellular vesicles in disease
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Connexins and lens biology
- Connective tissue disorders research
- Ocular Diseases and Behçet’s Syndrome
- Mosquito-borne diseases and control
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- melanin and skin pigmentation
- interferon and immune responses
- RNA regulation and disease
- Immune cells in cancer
- Ocular Surface and Contact Lens
- Traumatic Ocular and Foreign Body Injuries
- Retinal and Optic Conditions
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Restraint-Related Deaths
- Cell Image Analysis Techniques
- Corneal Surgery and Treatments
- Genomics and Rare Diseases
- Child Abuse and Related Trauma
Pennsylvania State University
2020-2025
Penn State Milton S. Hershey Medical Center
2018-2024
Michigan Medicine
2024
W.K. Kellogg Foundation
2018-2021
University of Michigan
2018-2021
Michigan United
2020-2021
Hershey (United States)
2018
Purpose: Current evidence suggests that retinal neurodegeneration is an early event in the pathogenesis of diabetic retinopathy. Our main goal was to examine whether, human retina, common proteins and pathways are shared with brain neurodegenerative diseases. Methods: A proteomic analysis performed on three groups postmortem retinas matched by age: nondiabetic control (n = 5), without glial activation 5). Retinal lysates from each group were pooled run SDS-PAGE gel. Bands analyzed...
To investigate the molecular components of vitreous in order to better understand retinal physiology and disease.Vitreous was acquired from patients undergoing vitrectomy for macular hole and/or epiretinal membrane, postmortem donors, C57BL/6J mice. Unbiased proteomic analysis performed via electrospray ionization tandem mass spectrometry (MS/MS). Gene ontology results were confirmed with transmission electron microscopy, atomic force nanoparticle tracking (NTA).Proteomic obtained prior...
Purpose: To investigate the role of protein misfolding in retinal pigment epithelial (RPE) cell dysfunction, effects R345W-Fibulin-3 expression on RPE phenotype were studied. Methods: Primary cells cultured to confluence Transwells and infected with lentivirus constructs express wild-type (WT)- or R345W-Fibulin-3. Barrier function was assessed by evaluating zonula occludens-1 (ZO-1) distribution trans-epithelial electrical resistance (TER). Polarized secretion vascular endothelial growth...
We have shown previously that expression of R345W-Fibulin-3 induces epithelial-mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells. The purpose the current study was to determine if extracellular vesicles (EVs) derived from RPE cells expressing mutation are sufficient induce EMT recipient ARPE-19 were infected with luciferase-tagged wild-type (WT)- Fibulin-3 or (R345W) using lentiviruses. EVs isolated media by ultracentrifugation density gradient ultracentrifugation....
Abstract Background Vitreous is an accessible, information-rich biofluid that has recently been studied as a source of retinal disease-related proteins and pathways. However, the number samples required to confidently identify perturbed pathways remains unknown. In order these pathways, power analysis must be performed determine required, sample preparation rigorously defined. Methods Control (n = 27) proliferative diabetic retinopathy 23) vitreous were treated biologically distinct...
Due to the close relationship between vitreous and posterior eye layers, microenvironment of these layers can affect composition vitreous. Molecular analysis may therefore provide important insights into pathogenesis chorioretinal diseases. In this study, cytokines (n = 41) were evaluated gain further tumor in uveal melanoma (UM) arising from choroid (CM). Cytokine levels measured using a bead-based multiplex immunoassay panel samples obtained 32 eyes, including 18 with CM 14 controls....
Bardet-Biedl syndrome (BBS), an autosomal recessive ciliopathy with pleiotropic effects, manifests as a spectrum of anomalies involving multiple genes and affects fewer than 3,000 individuals in the USA. Due to its rarity phenotypic variability, early diagnosis BBS poses significant challenge. Therefore, we aim shed light on intrafamilial variation resulting from
Diabetic retinopathy (DR) remains a leading cause of blindness in developed countries. Current treatments target late stages DR when vision has already been affected. The current study used an unbiased proteomic approach to gain better understanding early pathogenesis prior clinically detectable microvascular lesions. During these stages, neurodegeneration occurs and is marked by glial activation throughout the neuroretina. As increases, fibrillary acidic protein (GFAP) upregulated can be...
ABSTRACT Purpose Previous studies in our lab found that expression of R345W-Fibulin-3 induces retinal pigment epithelial (RPE) cells to undergo epithelial-mesenchymal transition (EMT). The purpose the current study was investigate size, cargo and function extracellular vesicles (EVs) derived from RPE expressing wild-type (WT)-Fibulin-3 compared mutation, determine role these EVs regulating cell dysfunction. Methods ARPE-19 were infected with luciferase-tagged Fibulin-3 (WT)- or (mutant)...