A5029593209- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Cancer-related gene regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Gut microbiota and health
- Chronic Myeloid Leukemia Treatments
- Diet and metabolism studies
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Cancer Genomics and Diagnostics
- Acute Lymphoblastic Leukemia research
- Liver Disease Diagnosis and Treatment
- Mesenchymal stem cell research
- Zebrafish Biomedical Research Applications
- T-cell and B-cell Immunology
- RNA Research and Splicing
The University of Tokyo
2007-2025
Tokyo University of Science
2003-2024
Institute for Stem Cell Biology and Regenerative Medicine
2018-2024
Chiba University
2014-2023
Regenerative Medicine Institute
2018-2019
MED Institute
2019
Tokyo Medical University
2019
Japan Science and Technology Agency
2002-2016
Weatherford College
2012
Science and Technology Corporation (United States)
2012
This study describes comprehensive polling of transcription start and termination sites analysis previously unidentified full-length complementary DNAs derived from the mouse genome. We identify 5' 3' boundaries 181,047 transcripts with extensive variation in arising alternative promoter usage, splicing, polyadenylation. There are 16,247 new protein-coding transcripts, including 5154 encoding proteins. Genomic mapping transcriptome reveals transcriptional forests, overlapping on both...
Recent advances in stem cell biology enable us to identify cancer cells solid tumors as well putative normal organs. In this study, we applied side population (SP) analysis and sorting established hepatocellular carcinoma (HCC) lines detect subpopulations that function elucidate their roles tumorigenesis. Among four analyzed, SP were detected Huh7 (0.25%) PLC/PRF/5 (0.80%), but not HepG2 Huh6 cells. demonstrated high proliferative potential anti-apoptotic properties compared with those of...
The process through which multipotential hematopoietic cells commit to distinct lineages involves the induction of specific transcription factors. PU.1 (also known as Spi-1) and GATA-1 are factors essential for development myeloid erythroid lineages, respectively. Overexpression can block differentiation in they normally down-regulated, indicating that not only positive but negative regulation these plays a role normal lineage development. Here we demonstrate region Ets domain (the winged...
Obesity increases the risk of cancers, including hepatocellular carcinomas (HCC). However, precise molecular mechanisms through which obesity promotes HCC development are still unclear. Recent studies have shown that gut microbiota may influence liver diseases by transferring its metabolites and components. Here, we show hepatic translocation obesity-induced lipoteichoic acid (LTA), a Gram-positive microbial component, creating tumor-promoting microenvironment. LTA enhances...
CCAAT/enhancer binding protein α (C/EBPα) is an integral factor in the granulocytic developmental pathway, as myeloblasts from C/EBPα-null mice exhibit early block differentiation. Since deficient for known C/EBPα target genes do not same granulocyte maturation, we sought to identify additional essential myeloid cell development. To such genes, used both representational difference analysis and oligonucleotide array with RNA derived a C/EBPα-inducible line. From each of these independent...
The polycomb group (PcG) protein Bmi1 plays an essential role in the self-renewal of hematopoietic and neural stem cells. Derepression Ink4a/Arf gene locus has been largely attributed to Bmi1-deficient phenotypes nervous system. However, its cell (HSC) remained undetermined. In this study, we show that derepressed p16Ink4a p19Arf mice were tightly associated with a loss self-renewing HSCs. deletion both Ink4a Arf genes substantially restored capacity Bmi1−/− Thus, regulates HSCs by acting as...
GATA transcription factors are major regulators of hematopoietic and immune system. Among factors, GATA-1, GATA-2, GATA-3 play crucial roles in the development erythroid cells, stem, progenitor T helper type 2 (Th2) respectively. A high level GATA-1 GATA-2 expression has been observed eosinophils, but their eosinophil remain uncertain both vitro vivo. Here we show that enforced human primary myeloid cells completely switches cell fate into eosinophils. Expression exclusively promotes...
Monocytic leukemia zinc-finger protein (MOZ), a MYST family histone acetyltransferase, is involved in the chromosome translocations associated with acute myeloid leukemia. MOZ acts as transcriptional coactivator for AML1, which essential establishment of definitive hematopoiesis. To investigate roles normal hematopoiesis, we generated MOZ-null mice. −/− mice died around embryonic day 15 (E15). In E14.5 embryos, hematopoietic stem cells, lineage-committed progenitors, and B lineage cells were...
Side population (SP) cell analysis and sorting have been successfully applied to hepatocellular carcinoma (HCC) lines identify a minor with cancer stem properties. However, the molecular mechanisms operating in SP cells remain unclear. The polycomb gene product BMI1 plays central role self-renewal of somatic variety tissues organs seems be implicated tumor development. In this study, we determined critical maintenance phenotype HCC lines. was preferentially expressed Huh7 PLC/PRF/5 compared...
Common molecular machineries between hematopoietic stem cell (HSC) maintenance and leukemia prevention have been highlighted. The tumor suppressor Fbxw7 (F-box WD-40 domain protein 7), a subunit of an SCF-type ubiquitin ligase complex, induces the degradation positive regulators cycle. We demonstrate that inactivation in cells causes premature depletion HSCs due to active cycling p53-dependent apoptosis. Interestingly, deletion also confers selective advantage with suppressed p53 function,...