A5004662093- Lung Cancer Research Studies
- Hematopoietic Stem Cell Transplantation
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- Bone health and treatments
- CAR-T cell therapy research
- Neuroblastoma Research and Treatments
- Viral-associated cancers and disorders
- Cell death mechanisms and regulation
- Lymphoma Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Hematological disorders and diagnostics
- Peripheral Neuropathies and Disorders
- CRISPR and Genetic Engineering
- Immunodeficiency and Autoimmune Disorders
- Immune cells in cancer
- Microbial Community Ecology and Physiology
- T-cell and B-cell Immunology
- Bone Metabolism and Diseases
- Protist diversity and phylogeny
The University of Tokyo
2013-2025
University of Tsukuba
2022-2024
University of York
2024
Stanford University
2015-2023
Nihonkai General Hospital
2011
Kobe City Medical Center General Hospital
2009-2010
Kanazawa University
2006
Kazusa DNA Research Institute
1996-2000
Kyoto University
1999
The sequence determination of the entire genome Synechocystis sp. strain PCC6803 was completed. total length finally confirmed 3,573,470 bp, including previously reported 1,003,450 bp from map position 64% to 92% genome. assembled sequences physical map-based contigs cosmid clones and lambda long PCR products which were used for gap-filling. accuracy guaranteed by analysis both strands DNA through authenticity supported restriction products, directly amplified genomic using data. To predict...
The sequence determination of the entire genome Synechocystis sp. strain PCC6803 was completed. total length finally confirmed 3,573,470 bp, including previously reported 1,003,450 bp from map position 64% to 92% genome. assembled sequences physical map-based contigs cosmid clones and λ long PCR products which were used for gap-filling. accuracy guaranteed by analysis both strands DNA through authenticity supported restriction products, directly amplified genomic using data. To predict...
Abstract Foxp3 + regulatory T cells (Treg cells) modulate the immune system and maintain self-tolerance, but whether they affect haematopoiesis or haematopoietic stem cell (HSC)-mediated reconstitution after transplantation is unclear. Here we show that B-cell lymphopoiesis impaired in Treg-depleted mice, yet this reduced rescued by adoptive transfer of affected HSCs bone marrow into Treg-competent recipients. abrogated both syngeneic allogeneic using mice as Treg can control physiological...
We demonstrated previously that membrane depolarization and excitatory receptor agonists such as noradrenaline induce Ca2+-dependent Rho activation in VSM (vascular smooth muscle), resulting MP (myosin phosphatase) inhibition through the mechanisms involving kinase-mediated phosphorylation of its regulatory subunit MYPT1. In present study, we show de-endothelialized strips PI3K (phosphoinositide 3-kinase) inhibitors LY294002 wortmannin inhibited KCl depolarization- noradrenaline-induced...
Advanced breast cancer is frequently associated with skeletal metastases and accelerated bone loss. Recombinant parathyroid hormone [teriparatide, PTH(1-34)] the first anabolic agent approved in US for treatment of osteoporosis. While signaling through PTH receptor osteoblast lineage regulates marrow hematopoietic niches, effects on metastatic niche are unknown. Here, we demonstrate, using orthotopic intratibial models 4T1 murine MDA-MB-231 human tumors, that decreases both tumor engraftment...
Gene editing using engineered nucleases frequently produces unintended genetic lesions in hematopoietic stem cells (HSCs). Gene-edited HSC cultures thus contain heterogeneous populations, the majority of which either do not carry desired edit or harbor unwanted mutations. In consequence, transplanting edited HSCs carries risks suboptimal efficiency and mutations graft. Here, we present an approach for expanding gene-edited at clonal density, allowing profiling individual clones before...
Abstract Small cell lung cancer (SCLC) is exceptionally aggressive, with limited treatment options. Disialoganglioside (GD2) highly expressed on SCLC and considered a good target for chimeric antigen receptor (CAR) T cells (CART). Although GD2-directed CARTs (GD2-CART) exhibit cytotoxicity against various GD2-expressing tumors, they lack significant SCLC. To enhance of GD2-CARTs SCLC, we introduced GD2-CAR into induced pluripotent stem (iPSC)-derived rejuvenated cytotoxic lymphocytes...
Bacillus cereus is a growing concern as cause of life-threatening infections in patients with hematologic malignancies. However, the risk factors for unfavorable outcomes have not been fully elucidated. At our institution, we observed growth B. blood culture 68 (23) or without (45) malignancies treated from September 2002 to November 2009. We defined case having sepsis when more than two sets were positive only single set was absence other microorganisms who had definite infectious lesions....
Bone metastases are a common complication of breast cancer. We have demonstrated that intermittent administration parathyroid hormone (PTH[1-34]) reduces the incidence bone in murine models cancer by acting on osteoblasts to alter microenvironment. Here, we examined role signaling mediated PTH 1 receptor (PTH1R) both and cells influencing metastases. In mice with impaired PTH1R osteoblasts, did not reduce metastasis. Intermittent also metastasis when expression was knocked down 4T1 shRNA....
Patients with permanent hypoparathyroidism require lifelong replacement therapy to avoid life-threatening complications, The benefits of conventional treatment are limited, however. Transplanting a functional parathyroid gland (PTG) would yield better results. Parathyroid cells generated from pluripotent stem in vitro date cannot mimic the physiological responses extracellular calcium that essential for homeostasis. We thus hypothesized blastocyst complementation (BC) could be strategy...
A 55-year-old woman was diagnosed with POEMS syndrome; however, we could not perform high-dose chemotherapy autologous peripheral blood stem cell transplantation (auto-PBSCT) because of rapidly progressive congestive heart failure. Thus, treated the patient thalidomide plus dexamethasone, which brought about a marked improvement systolic dysfunction, syndrome-related symptoms and serum concentrations vascular endothelial growth factor. Subsequently, safely performed auto-PBCST. This report...
Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal (MSCs) on differentiation HSPCs and progenitors through C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα+Sca-1+ (PαS cells), were found secrete CCL2, particularly response lipopolysaccharide stimulation. MSC-secreted CCL2 promoted granulocyte/macrophage into myeloid...