A5043066178- Planarian Biology and Electrostimulation
- Marine Ecology and Invasive Species
- Biocrusts and Microbial Ecology
- Tardigrade Biology and Ecology
- Reproductive biology and impacts on aquatic species
- Sperm and Testicular Function
- Marine Biology and Environmental Chemistry
- Plant and Biological Electrophysiology Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Reproductive Biology and Fertility
- Echinoderm biology and ecology
- bioluminescence and chemiluminescence research
- Cephalopods and Marine Biology
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Anesthesia and Pain Management
- Protist diversity and phylogeny
- Marine and coastal plant biology
- Cardiac, Anesthesia and Surgical Outcomes
- Photoreceptor and optogenetics research
- Insect and Arachnid Ecology and Behavior
- Biofield Effects and Biophysics
- Invertebrate Immune Response Mechanisms
- Chemical Reactions and Isotopes
- Cardiac Ischemia and Reperfusion
Keio University
2012-2024
Keio University Shonan Fujisawa
2019
Science and Technology Interactive Center
2015
Tachikawa Hospital
2000-2009
Remote Sensing Technology Center of Japan
2004
Kazusa DNA Research Institute
2000-2003
Tokyo Institute of Technology
1992-1999
Mitsubishi Group (Japan)
1990-1999
Keio University Hospital
1998
Sumitomo Precision Products (Japan)
1998
The complete nucleotide sequence of the genome a symbiotic bacterium Bradyrhizobium japonicum USDA110 was determined. B. single circular chromosome 9,105,828 bp in length with an average GC content 64.1%. No plasmid detected. comprises 8317 potential protein-coding genes, one set rRNA genes and 50 tRNA genes. Fifty-two percent protein showed similarity to known function 30% hypothetical remaining 18% had no apparent reported Thirty-four significant those both Mesorhizobium loti Sinorhizobium...
Abstract Background In gonochoristic animals, the sex determination pathway induces different morphological and behavioral features that can be observed between sexes, a condition known as sexual dimorphism. While many components of this differentiation cascade show high levels diversity, factors such Doublesex-Mab-3-Related Transcription factor (DMRT) are widely conserved across animal taxa. Species phylum Tardigrada exhibit remarkable diversity in morphology behavior suggesting regulating...
It has been known for a long time that heme oxygenase (HO) is key enzyme in catabolism, and it was found to act as an oxidative‐stress protein produce carbon monoxide, which similar actions those of nitrogen monoxide. We examined transcriptional control the HO gene mouse M1 (myeloleukemia) cells during treatment with lipopolysaccharide (LPS; oxidative reagent). Since promoter region this human contains 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate(TPA)‐responsive element (TRE)...
Abstract Aim Freshwater planarians may have a wide geographical range despite their assumed low vagility. Found across four continents, Dugesia either an ancient origin on large palaeo landmass, followed by colonisation in different regions before continental fragmentation, or more recent and subsequent transoceanic dispersal. We seek to resolve between these two hypotheses. Location Africa, Eurasia Australasia. Taxon Genus (Platyhelminthes: Tricladida: Dugesiidae). Methods used data from...
Starfish‐oocyte maturation induced by 1‐methyladenine (MeAde) was inhibited microinjection of pertussis toxin (PTX). The inhibition appeared to result from PTX‐catalyzed ADP‐ribosylation a 39‐kDa guanosine‐nucleotide‐binding regulatory protein (G protein) in the oocyte. These results strongly support hypothesis that MeAde‐induced signals operate via membrane receptor and are carried PTX‐sensitive G protein. When PTX‐injected oocytes were treated with dithiothreitol, 85% them reinitiated...
Boreal ecosystems play an essential role in global climate regulation. Forests constitute pools of terrestrial carbon and are generally considered as sinks atmospheric CO/sub 2/, contributing to attenuating the greenhouse effect. Large amounts also stored boreal lakes, bogs wetlands, partially released CH/sub 4/ other trace gases atmosphere during spring summer months. Human activities forest zone however reducing size pool change is triggering shorter winters earlier thaw onset, changing...
Oocyte maturation and subsequent spawning in hydrozoan jellyfish are generally triggered by light-dark cycles. To examine if the initiation of process after light stimulus is mediated neurotransmitters, neuropeptides isolated originally from Hydra magnipapillata were applied to sexually mature female medusae Cytaeis uchidae. Among tested, Hym-53 (NPYPGLW-NH2 ), as well a nonphysiological peptide, CGLWamide (CGLW-NH2 most effective inducing oocyte spawning. Hym-355 (FPQSFLPRG-NH2 ) also these...
An assay system has been established for the sexual induction in OH strain, an exclusively fissiparous (asexual) of Dugesia ryukyuensis by feeding them with sexually matured worms Bdellocephala brunnea, oviparous (sexual) species. In this system, asexual gradually differentiated organs, namely ovary, testis, genital pore and yolk gland order, eventually mated laid cocoons filled fertilized eggs. Although strain were believed not to have any a pair undeveloped ovaries few oogonia detected...
Reproductive strategy is an important aspect of biological diversity. In tardigrades, several reproductive modes, including sexual reproduction, are known. However, tardigrade mating behavior has been observed only rarely in most species, and some cases, especially the freely ovipositing eutardigrades, remains entirely unknown. present study, we cultured two sexually reproducing species that lay eggs freely, Paramacrobiotus sp. TYO strain Macrobiotus shonaicus, to investigate compare their...
Tardigrades are microscopic organisms, famous for their tolerance against extreme environments. The establishment of rearing systems multiple species has allowed comparison tardigrade physiology, in particular embryogenesis. Interestingly, in-lab cultures limnic showed smaller variation hatching timing than terrestrial species, suggesting a regulation mechanism acquired by adaptation to habitat.To this end, we screened coordinated gene expression during the development two tardigrades,...
Spermatozoa bind to the vitelline coat in ascidians and many other animals. The binding of sperm Halocynthia roretzi is mediated by a α-l-fucosidase complementary-l-fucosyl residues glycoproteins coat. cDNA clones for were isolated from growing testis mRNA. It contained 1398 bp full-length insert (HrFuc'ase) that encoded 466 amino acid H. α-l-fucosidase. A putative signal peptide 21 proceeded sequence mature protein (M.W. 52.4 kDa). coding HrFuc'ase showed 47.7% identity human liver...
The planarian Dugesia ryukyuensis reproduces both asexually and sexually, can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching asexual sexual mode. constructed cDNA library sexualized D. sequenced analyzed 8,988 expressed sequence tags (ESTs). ESTs were grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed basis unigene sets. Fifty-six percent cDNAs shared similarity (E-value<1E −20) with sequences...
Homologs of <I>nanos</I> are required for the formation and maintenance germline stem cell (GSC) systems gametogenesis in many metazoans. Planarians can change their reproductive mode seasonally, alternating between asexual sexual reproduction; they develop maintain somatic cells (SSCs) GCSs from pluripotent known as neoblasts. We isolated a homolog, <I>Dr-nanos</I>, expressed sequence tags (ESTs) sexualized form <I>Dugesia ryukyuensis</I>. examined expression of<I> Dr-nanos </I>in...
Hsc70t is a member of the Hsp70 family genes and constitutively expressed after meiosis in mouse spermatogenesis. Immunohistochemistry situ hybridization techniques were used to examine precise localization product during various stages A rabbit antiserum raised against Hsc70t-lacZ fusion protein detected late spermatid-enriched fraction two-dimensional Western blot analyses. On histological sections, appears cytoplasm spermatids as they progress from step 9 final An antisense RNA probe...
The genus Paramacrobiotus was erected in 2009 from the Macrobiotus, and 43 species have been described to date. Although first genome sequence reported for TYO strain of sp., which is a dioecious has five bivalent chromosomes, its precise taxonomic identification remained undetermined. Here, we report morphology, confirming presence microplacoid, cuticular bulge on inner side legs IIII, granulation IV under both light electron microscopy, smooth areoles egg shell, indicating that it differs...