A5004340414- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Supramolecular Chemistry and Complexes
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Extraction and Separation Processes
- Vector-Borne Animal Diseases
- Radioactive element chemistry and processing
- Crystallography and molecular interactions
- Organophosphorus compounds synthesis
- Molecular Sensors and Ion Detection
- Protist diversity and phylogeny
- Carbohydrate Chemistry and Synthesis
- Metal-Organic Frameworks: Synthesis and Applications
- Animal Virus Infections Studies
- Legume Nitrogen Fixing Symbiosis
- Analytical chemistry methods development
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Nonlinear Optical Materials Research
- Lanthanide and Transition Metal Complexes
- Immune Response and Inflammation
- Phosphorus compounds and reactions
- Microbial infections and disease research
- Synthesis of Organic Compounds
- Chemical Synthesis and Characterization
Akita University
2015-2024
National Agriculture and Food Research Organization
2013-2023
National Institute of Animal Health
2013-2023
Kazusa DNA Research Institute
2008-2023
Graduate School USA
2021
Kyoto Medical Center
2018
Breakthrough
2018
Shizuoka University
1981-2016
Culture Resource
2014-2016
Shoei Junior College
2016
The sequence determination of the entire genome Synechocystis sp. strain PCC6803 was completed. total length finally confirmed 3,573,470 bp, including previously reported 1,003,450 bp from map position 64% to 92% genome. assembled sequences physical map-based contigs cosmid clones and lambda long PCR products which were used for gap-filling. accuracy guaranteed by analysis both strands DNA through authenticity supported restriction products, directly amplified genomic using data. To predict...
The sequence determination of the entire genome Synechocystis sp. strain PCC6803 was completed. total length finally confirmed 3,573,470 bp, including previously reported 1,003,450 bp from map position 64% to 92% genome. assembled sequences physical map-based contigs cosmid clones and λ long PCR products which were used for gap-filling. accuracy guaranteed by analysis both strands DNA through authenticity supported restriction products, directly amplified genomic using data. To predict...
The complete nucleotide sequence of the genome a symbiotic bacterium Bradyrhizobium japonicum USDA110 was determined. B. single circular chromosome 9,105,828 bp in length with an average GC content 64.1%. No plasmid detected. comprises 8317 potential protein-coding genes, one set rRNA genes and 50 tRNA genes. Fifty-two percent protein showed similarity to known function 30% hypothetical remaining 18% had no apparent reported Thirty-four significant those both Mesorhizobium loti Sinorhizobium...
The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such symbiotic nitrogen fixation.Here, we report structural features L. genome.The 315.1-Mb sequences determined in this and previous studies correspond 67% genome (472 Mb), are likely cover 91.3% gene space.Linkage mapping anchored 130-Mb onto six linkage groups.A total 10 951 complete 19 848 partial structures protein-encoding genes were assigned...
Between 2 and 44 gL−1 of the diamide 1 are necessary to obtain stable gels with a wide variety organic solvents. The circular dichroism can be attributed formation chiral aggregates from 1, which held together by hydrogen bonds van der Waals interactions.
Cultivated strawberry (Fragaria x ananassa) is octoploid and shows allogamous behaviour. The present study aims at dissecting this genome through comparison with its wild relatives, F. iinumae, nipponica, nubicola, orientalis by de novo whole-genome sequencing on an Illumina Roche 454 platforms. total length of the assembled sequences obtained was 698 Mb for ananassa, ∼200 each four species. Subsequently, a virtual reference termed FANhybrid_r1.2 constructed integrating homoeologous...
The nucleotide sequence of the complete genome a cyanobacterium, Microcystis aeruginosa NIES-843, was determined. M. is single, circular chromosome 5 842 795 base pairs (bp) in length, with an average GC content 42.3%. comprises 6312 putative protein-encoding genes, two sets rRNA 42 tRNA genes representing 41 species, and for tmRNA, B subunit RNase P, SRP RNA, 6Sa RNA. Forty-five percent sequences showed similarity to known function, 32% were similar hypothetical remaining 23% had no...
We determined the nucleotide sequence of entire genome a diazotrophic endophyte, Azospirillum sp.B510.Strain B510 is an endophytic bacterium isolated from stems rice plants (Oryza sativa cv.Nipponbare).The consisted single chromosome (3 311 395 bp) and six plasmids, designated as pAB510a (1 455 109 bp), pAB510b (723 779 pAB510c (681 723 pAB510d (628 837 pAB510e (537 299 pAB510f (261 596 bp).The bears 2893 potential protein-encoding genes, two sets rRNA gene clusters (rrns), 45 tRNA genes...
Abstract Graphene is an interesting two‐dimensional carbon sheet possessing single‐layered atom thickness that confers unique physical and chemical properties. The pristine graphene sheets have some limited applications in water purification, but the modification of into composite by incorporation functional groups or nanoparticles on surface extensively increases its environmental applications. Recently, nanocomposites found to show very promising all types purification. present review...
We generated a high-density genetic linkage map of soybean using expressed sequence tag (EST)-derived microsatellite markers. A total 6920 primer pairs (10.9%) were designed to amplify simple repeats (SSRs) from 63 676 publicly available non-redundant ESTs. The polymorphism two parent plants, the Japanese cultivar 'Misuzudaizu' and Chinese line 'Moshidou Gong 503', examined 10% polyacrylamide gel electrophoresis. Primer showing then used for genotyping 94 recombinant inbred lines (RILs)...
Abstract Background Biological nitrogen fixation is a prokaryotic process that plays an essential role in the global cycle. Azorhizobium caulinodans ORS571 has dual capacity to fix both as free-living organism and symbiotic interaction with Sesbania rostrata . The host fast-growing, submergence-tolerant tropical legume on which A. can efficiently induce nodule formation root system adventitious rootlets located stem. Results 5.37-Mb genome consists of single circular chromosome overall...
Despite the collection and availability of abundant tomato genome sequences, PCR-based markers adapted to large scale analysis have not been developed in species. Therefore, using public sequence data tomato, we three types DNA markers: expressed tag (EST)-derived simple repeat (SSR) (TES markers), genome-derived SSR (TGS markers) EST-derived intronic polymorphism (TEI markers). A total 2,047 TES, 3,510 TGS 674 TEI were established used polymorphic a cultivated (Solanum lycopersicum) 'LA925'...
Large-scale development of expressed sequence tag simple repeat (EST-SSR) markers was performed in peanut (Arachis hypogaea L.) to obtain more informative genetic markers. A total 10,102 potential non-redundant EST sequences, including 3,445 contigs and 6,657 singletons, were generated from cDNA libraries the gynophore, roots, leaves seedlings. 3,187 primer pairs designed on flanking regions SSRs, some which allowed one two base mismatches. Among generated, 2,540 (80%) trinucleotide repeats,...
In order to upgrade the genome sequence information of J. curcas L., we integrated de novo assembly a total 537 million paired-end reads generated from Illumina sequencing platform into current which was obtained by combination conventional Sanger method and Roche/454 platform. The length upgraded sequences thus 297,661,187 bp consisting 39,277 contigs. average N50 lengths contigs were 7,579 15,950 bp, both increased fourfold previous assembly. Along with upgrading, currently available...
Currently, in mass spectrometry-based metabolomics, limited reference spectra are available for flavonoid identification. In the present study, a database of probable fragments 6,867 known flavonoids (FsDatabase) was manually constructed based on new structure- and fragmentation-related rules using heuristics to overcome complexity. We developed FlavonoidSearch system annotation, which consists FsDatabase computational tool (FsTool) automatically search metabolite peaks as queries. This...
Previous studies indicated that inorganic pyrophosphatase of Ascaris suum (AsPPase) plays an important role in larval survival the host. Here we describe a precise for AsPPase molting and development also potential recombinant (rAsPPase) protective immunity to A. infection. Using reverse transcriptase PCR analysis, found disruption gene function by RNA interference resulted suppression mRNA levels. caused inhibition third-stage larvae (31%) native protein expression, as demonstrated 56%...
<italic>N</italic>-Ethyl quinolinium chromophore crystal 2-[2-(4-chloro-phenyl)-vinyl]-1-ethyl-quinolinium iodide (PCLQI) has been successfully grown by the slow evaporation technique.
Following an outbreak of classical swine fever (CSF) in Japan, 2018, CSFV JPN/1/2018 was isolated from infected pig sample. In this study, we carried out a comparative experimental infection pigs using strain and the highly virulent ALD compared outcomes, including clinical manifestation, virus shedding patterns antibody responses. Although inoculated orally or intramuscularly with developed hyperthermia had decreased leucocyte numbers, they survived for whole period showed less severe signs...
African swine fever (ASF) is an infectious Suidae disease caused by the ASF virus (ASFV). Adaptation to less susceptible, non-target host cells one of most common techniques used attenuate virulent viruses. However, this may induce many mutations and large-scale rearrangements in viral genome, resulting immunostimulatory potential loss vivo. This study continuously maintained ASFV strain, Armenia2007 (Arm07), establish attenuated strain with minimum genetic alteration a susceptible cell...
Zwischen 2 und 44 g L −1 des Diamids 1 werden nur benötigt, um mit einer großen Vielfalt organischer Lösungsmittel stabile Gele zu erhalten. Der Circulardichroismus der läßt sich auf die Bildung chiraler Aggregate aus zurückführen, durch Wasserstoffbrückenbindungen van‐der‐Waals‐Wechselwirkungen zusammengehalten werden. magnified image