- Microbial Community Ecology and Physiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Particle accelerators and beam dynamics
- Algal biology and biofuel production
- Particle Accelerators and Free-Electron Lasers
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Plant Pathogens and Fungal Diseases
- Superconducting Materials and Applications
- Mycorrhizal Fungi and Plant Interactions
- Infectious Diseases and Mycology
- Biocrusts and Microbial Ecology
- Neurological Disorders and Treatments
- Magnetic confinement fusion research
- Nematode management and characterization studies
- Biofuel production and bioconversion
- Environmental DNA in Biodiversity Studies
- Biodiesel Production and Applications
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Marine Toxins and Detection Methods
- Porphyrin Metabolism and Disorders
- Photosynthetic Processes and Mechanisms
- Analytic Number Theory Research
- Phytochemical and Pharmacological Studies
National Institute for Environmental Studies
2002-2024
University of Tsukuba
2012-2023
Kobe University
2019
Tohoku University
2013-2017
Tokyo Metropolitan Geriatric Hospital
2004
Toshiba (Japan)
1981-2002
The University of Tokyo
1996-2002
High Energy Accelerator Research Organization
2002
Wakayama Prefectural Tanabe High School
1987
The nucleotide sequence of the complete genome a cyanobacterium, Microcystis aeruginosa NIES-843, was determined. M. is single, circular chromosome 5 842 795 base pairs (bp) in length, with an average GC content 42.3%. comprises 6312 putative protein-encoding genes, two sets rRNA 42 tRNA genes representing 41 species, and for tmRNA, B subunit RNase P, SRP RNA, 6Sa RNA. Forty-five percent sequences showed similarity to known function, 32% were similar hypothetical remaining 23% had no...
After a general clinical observation period of over 4 weeks, 20 essential hypertensive subjects (Japanese) were randomly divided into two groups. One group (n = 10; men and 6 women; 51.4 +/- 2.8 years age) agreed to physical training using bicycle ergometer exercise with the intensity at blood lactate threshold for 60 minutes three times week 10 while other 51.0 2.9 did no particular was followed once as control. Changes in pressure, hemodynamics, humoral factors exercised compared values...
The Zygomycota is an ecologically heterogenous assemblage of nonzoosporic fungi comprising two classes, Zygomycetes and Trichomycetes. Phylogenetic analyses have suggested that the phylum polyphyletic; four orders Trichomycetes are related to Mesomycetozoa (protists) diverged near fungal/animal split. Current circumscription includes only with representatives produce zygospores. We present a molecular-based phylogeny including recognized combined dataset for nuclear rRNA 18S (SSU), 5.8S 28S...
Microcystis aeruginosa is a bloom-forming cyanobacterium found in eutrophic water bodies worldwide. M. blooms usually occur freshwater; however, they have also been reported to brackish water. Because often produces the cyanotoxin microcystin, are major concern public health and environment. Despite this, ecology, genomic basis, evolutionary process underlying bloom invasion from fresh poorly investigated. Hence, present study, we sequenced characterized genomes of two newly discovered...
Microcystis aeruginosa is one of the most prevalent bloom-forming cyanobacteria and has been cause increasing public health concern due to production hepatotoxins (microcystins). To investigate genetic diversity, clonality evolutionary background with regard toxicity M. aeruginosa, a multilocus sequence typing (MLST) scheme was developed, based on seven selected housekeeping loci (ftsZ, glnA, gltX, gyrB, pgi, recA tpi). Analysis collection 164 isolates from Japan other countries identified...
The Zygomycota is an ecologically heterogenous assemblage of nonzoosporic fungi comprising two classes, Zygomycetes and Trichomycetes. Phylogenetic analyses have suggested that the phylum polyphyletic; four orders Trichomycetes are related to Mesomycetozoa (protists) diverged near fungal/animal split. Current circumscription includes only with representatives produce zygospores. We present a molecular-based phylogeny including recognized combined dataset for nuclear rRNA 18S (SSU), 5.8S 28S...
Botryococcus braunii is a colony-forming green alga that accumulates large amounts of liquid hydrocarbons within the colony. The utilization B. for biofuel production however hindered by its low biomass productivity. Here we describe novel bacterial ectosymbiont (BOTRYCO-2) confers higher productivity to braunii. 16S rDNA analysis indicated sequence BOTRYCO-2 shows similarity (<90%) cultured species and located phylogenetic lineage consisting uncultured alphaproteobacterial clones....
Algae have contributed greatly to the creation of Earth's environment and development human civilization. Currently, microalgae are considered be among most promising sources for biofuel. Most accumulate triacylglycerols; however, fatty acid methyl esters produced from triacylglycerols by transesterification critical end-use issues. Hydrocarbons Botryococcus Aurantiochytrium suitable algal oils replacing existing transportation fuels highly compatible with petroleum infrastructure. Over...
The water-bloom-forming cyanobacterium Microcystis aeruginosa is a known producer of various kinds toxic and bioactive chemicals. Of these, hepatotoxic cyclic heptapeptides microcystins have been studied most intensively due to increasing concerns for human health risks environmental damage. More than 70 variants are known, single microcystin synthetase (mcy) gene cluster consisting 10 genes (mcyA mcyJ) has identified be responsible the production all microcystins. Our previous multilocus...
In previous studies, we have demonstrated that the population structure of bloom-forming cyanobacterium Microcystis aeruginosa is clonal. Expanded multilocus sequence typing analysis M. using 412 isolates identified five intraspecific lineages suggested to be panmictic while maintaining overall clonal probably due a reduced recombination rate between lineages. Interestingly, since 2005 most strains belonging one these clusters (group G) been found in particular locality (Lake Kasumigaura...
SUMMARY Previous polyphasic analyses of five morphospecies the water‐bloom‐forming cyanobacterial genus Microcystis , aeruginosa (Kützing) Lemmermann (= Kützing), ichthyoblabe Kützing, novacekii (Komárek) Compère, viridis (A. Braun) Lemmermann, and wesenbergii Komárek in Kondratieva, have shown them to be conspecific they been proposed included under binomial Lemmermann. However, several from tropical regions, such as bengalensis Banerji, panniformis Komárek, Komárková‐Legnerová, Sant'anna,...
Microcystis aeruginosa NIES-2549 is a freshwater bloom-forming cyanobacterium isolated from Lake Kasumigaura, Japan. We report the complete 4.29-Mbp genome sequence of and its annotation discuss genetic diversity M. strains. This third Kasumigaura.
Abstract Marine bacterial lineages associated with algal blooms, such as the Roseobacter clade, have been well characterized in ecological and genomic contexts, yet rarely explored freshwater blooms. This study performed phenotypic analyses of an alphaproteobacterial lineage ‘Candidatus Phycosocius’ (denoted CaP clade), one few ubiquitously described a novel species: ‘Ca. Phycosocius spiralis.’ Phylogenomic indicated that clade is deeply branching Caulobacterales. Pangenome revealed...
Nanda Kyaw Thu, Tanabe Y., Yoshida M., Matsuura H. and Watanabe M.M. 2012. Aerosakkonema funiforme gen. et sp. nov. (Oscillatoriales), a new gas-vacuolated oscillatorioid cyanobacterium isolated from mesotrophic reservoir. Phycologia 51: 672–683. DOI: 10.2216/11-130.1A novel was the Nam Ngum Reservoir, Lao People's Democratic Republic. Five strains were tentatively assigned to this because they showed morphological characteristics different previously described freshwater species of...
Aphelids (phylum Aphelida = Aphelidiomycota) are intracellular parasitoids of algae and represent one the early-diverging or sister lineages kingdom Fungi. Although aphelids a small group comprising four genera 17 species, molecular phylogenetic analyses revealed that numerous environmental DNA sequences undescribed lineages, indicating their hidden diversity. Here, we investigated novel aphelid strain, KS114, parasitizes selenastracean green algae. KS114 exhibited life cycle typical...
A DNA fragment that caused pigment production in Streptomyces lividans was isolated from a gene library of PstI-digested chromosomal fragments S. coelicolor A3(2). Subcloning and nucleotide sequencing proved the identity cloned to ptpA encoding low-molecular-mass phosphotyrosine protein phosphatase. The transformant containing on pIJ41 with copy number 3–4 per genome produced large amounts undecylprodigiosin A-factor, addition pigmented antibiotic actinorhodin, whereas an SCP2* derivative...