A5058748014- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Adipose Tissue and Metabolism
- Cardiac pacing and defibrillation studies
- Cardiac Ischemia and Reperfusion
- Cardiomyopathy and Myosin Studies
- Osteoarthritis Treatment and Mechanisms
- Gastric Cancer Management and Outcomes
- Esophageal Cancer Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Pancreatic and Hepatic Oncology Research
- ECG Monitoring and Analysis
- Neuroendocrine Tumor Research Advances
- Algal biology and biofuel production
- Neuropeptides and Animal Physiology
- Cell Adhesion Molecules Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Congenital heart defects research
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiac Arrhythmias and Treatments
- Anesthesia and Sedative Agents
Shiga University of Medical Science
2016-2025
Aichi University of Technology
2023-2024
Mitsubishi Tanabe Pharma Corporation
2023
Tokai University
2023
Shiga University
2004-2022
Saiseikai Fukuoka General Hospital
1997-2021
NTT Medical Center
2018
University of Tsukuba
2011-2015
Taisho Pharmaceutical (Japan)
2013
Japan Society for the Promotion of Science
2013
Here we report on the 18W-13a strain of Aurantiochytrium sp., which accumulates very high amounts squalene. The squalene contents and production at 4 d culture were 198 mg/g 1.29±0.13 g/L, respectively, exceptionally values compared to previous reports.
Brugada syndrome (BrS) has a significantly higher incidence among the male sex. Among genes coding ion channels and their modulatory proteins, KCNE5 (KCNE1L) is located in X chromosome encodes an auxiliary β-subunit for K channels. been shown to modify transient outward current (I(to)), which plays key role determining repolarization process myocardium. This study investigated whether mutations could be responsible BrS other idiopathic ventricular fibrillation (IVF).In 205 Japanese patients...
Drugs with I(Kr)-blocking action cause secondary long-QT syndrome. Several cases have been associated mutations of genes coding cardiac ion channels, but their frequency among patients affected by drug-induced syndrome (dLQTS) and the resultant molecular effects remain unknown.Genetic testing was carried out for syndrome-related in 20 subjects dLQTS 176 congenital (cLQTS); electrophysiological characteristics dLQTS-associated were analyzed using a heterologous expression system Chinese...
Background— Mutations of KCNJ2 , the gene encoding human inward rectifier potassium channel Kir2.1, cause Andersen-Tawil syndrome (ATS), a disease exhibiting ventricular arrhythmia, periodic paralysis, and dysmorphic features. However, some mutation carriers lack ATS triad sometimes share phenotype catecholaminergic polymorphic tachycardia (CPVT). We investigated clinical biophysical characteristics with “atypical ATS.” Methods Results— Mutational analyses were performed in 57 unrelated...
CACNA1C mutations have been reported to cause LQTS type 8 (LQT8; Timothy syndrome), which exhibits severe phenotypes, although the frequency of patients with LQT8 exhibiting only QT prolongation is unknown. This study aimed elucidate in long syndrome (LQTS), except those and investigate phenotypic variants.CACNA1C gene screening was performed 278 probands negative for LQTS-related mutations. Functional analysis mutant channels using a whole-cell patch-clamp technique also performed. Using...
Laminin-5, consisting of the α3, β3, and γ2 chains, is localized in skin basement membrane supports structural stability epidermo-dermal linkage regulates various cellular functions. The α chains laminins have been shown to biological activities. In this study, we identified a sequence α3 chain C-terminal globular domain (LG1-LG5 modules) required for both heparin binding cell adhesion using recombinant proteins synthetic peptides. We found that LG3 LG4 modules activity has adhesion. Studies...
Free radical-induced oxidant stress has been implicated in ischemia and reperfusion-induced injury the heart. A number of studies have reported that reduces activity isolated Na+,K(+)-ATPase enzyme. We studied effects on Na(+)-K+ pump current recorded rabbit ventricular myocytes using whole-cell voltage-clamp technique. Singlet oxygen superoxide were generated by photoactivation rose bengal (50 nM). The compositions Tyrode's pipette solutions designed to block channel currents electrogenic...
1. The hypothesis that the inwardly rectifying K+ channel consists of a triple‐barrel structure was investigated. Inward currents were recorded under blocking effects external Cs+ or Rb+ in cell‐attached configuration patch‐clamp technique using single ventricular cells enzymatically isolated from guinea‐pig hearts. 2. (10‐100 microM) (20‐100 added to 150 mM‐K+ pipette solution induced rapid open‐blocked transitions inward open‐channel currents. In about 20% experiments current showed two...
Botryococcus braunii is a colony-forming green alga that accumulates large amounts of liquid hydrocarbons within the colony. The utilization B. for biofuel production however hindered by its low biomass productivity. Here we describe novel bacterial ectosymbiont (BOTRYCO-2) confers higher productivity to braunii. 16S rDNA analysis indicated sequence BOTRYCO-2 shows similarity (<90%) cultured species and located phylogenetic lineage consisting uncultured alphaproteobacterial clones....
Background— Angiotensin II (Ang II) has diverse actions on cardiac electrical activity. Little information is available, however, regarding immediate electrophysiological effects of Ang repolarization. Methods and Results— The present study investigated the slow component delayed rectifier K + current ( I Ks ) action potentials in guinea pig atrial myocytes using whole-cell patch-clamp technique. Bath application increased amplitude (EC 50 , 6.16 nmol/L) concentration dependently. stable...
Single atrial cells from guinea pig heart were voltage-clamped using the whole-cell configuration of patch-clamp technique under conditions in which most ionic and exchange currents known cardiac minimized. Extracellular 5 or 50 microM ATP activated a Cl- current, addition to rapidly desensitizing cation-selective current. A nonhydrolyzable analogue, adenosine-5'-O-(3-thiotriphosphate) (50 microM), also evoked these two currents, indicating involvement purinoceptors rather than ecto-ATPase...