A5046015612- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Chemical Synthesis and Analysis
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Click Chemistry and Applications
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Photochromic and Fluorescence Chemistry
- Supramolecular Self-Assembly in Materials
- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Advanced Fluorescence Microscopy Techniques
- Adipose Tissue and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Biotin and Related Studies
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Cardiac electrophysiology and arrhythmias
- Advanced Biosensing Techniques and Applications
- Polydiacetylene-based materials and applications
- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
Kyoto University
2011-2025
Japan Science and Technology Agency
2009-2024
Nagoya University
2020-2024
SPring-8
2023
Graduate School USA
2013-2021
Kyoto Katsura Hospital
2005-2017
Fukuoka University
2010-2016
National Institutes of Natural Sciences
2016
Okazaki Institute for Integrative Bioscience
2016
National Institute for Physiological Sciences
2016
Canonical transient receptor potential (TRPC) channels control influxes of Ca(2+) and other cations that induce diverse cellular processes upon stimulation plasma membrane receptors coupled to phospholipase C (PLC). Invention subtype-specific inhibitors for TRPCs is crucial distinction respective TRPC play particular physiological roles in native systems. Here, we identify a pyrazole compound (Pyr3), which selectively inhibits TRPC3 channels. Structure-function relationship studies compounds...
Neurotransmitter receptors are essential components of synapses for communication between neurons in the brain. Because spatiotemporal expression profiles and dynamics neurotransmitter involved many functions delicately governed brain, vivo research tools with high resolution intact brains highly desirable. Covalent labeling by chemical reaction (chemical labeling) proteins without genetic manipulation is now a powerful method analyzing vitro. However, selective target receptor brain has not...
In materials science, a dynamic property sensitive to an environmental change (heat, light, electric current, pH, and other chemical or physical changes) is indispensable for intelligent materials. Such organic materials, however, are very limited even in conventional polymers. This paper clearly demonstrates that, regardless of the low molecular weight, glycosylated amino acid derivative newly screened by combinatorial method forms macroscopic supramolecular hydrogel that reversibly swells...
AbstractTRPA1 is a member of the transient receptor potential (TRP) cation channel family, and predominantly expressed in nociceptive neurons dorsal root ganglia (DRG) trigeminal ganglia. Activation TRPA1 by environmental irritants such as mustard oil, allicin, acrolein causes acute pain. However, endogenous ligands that directly activate remain elusive inflammation. Here, we show variety inflammatory mediators (15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), nitric oxide (NO), hydrogen...
To accomplish the selective labeling of a specific protein in complicated biological systems, peptide tag incorporated into and complementary small molecular probe are required. Although variety tag/probe pairs have been developed as tools for analyses, availability suitable real-time imaging proteins is still limited. We now report new tag/artificial pair composed genetically encodable oligo-aspartate sequence (D4 tag, (D4)n, n = 1-3) corresponding multinuclear Zn(II) complexes...
Atrial and brain natriuretic peptides (ANP BNP, respectively) exert antihypertrophic effects in the heart via their common receptor, guanylyl cyclase (GC)-A, which catalyzes synthesis of cGMP, leading to activation protein kinase (PK)G. Still, much network molecular mediators ANP/BNP-GC-A signaling inhibit cardiac hypertrophy remains be characterized.We investigated effect ANP-GC-A on transient receptor potential subfamily C (TRPC)6, a receptor-operated Ca(2+) channel known positively...
Point the finger: Zinc-finger proteins are convenient and site-selective adaptors for targeting specific locations within DNA-origami structures. Orthogonal of in structures was demonstrated by using two adaptors, application Escherichia coli lysate that contained adaptor-fused successfully afforded expected protein–DNA assembly.
Abstract The location and number of neurotransmitter receptors are dynamically regulated at postsynaptic sites. However, currently available methods for visualizing receptor trafficking require the introduction genetically engineered into neurons, which can disrupt normal functioning processing original receptor. Here we report a powerful method native α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate (AMPARs) essential cognitive functions without any genetic...
A pH-responsive volume-change function was successfully introduced into a supramolecular hydrogel that contained GalNAc-appended (GalNAc=N-acetylgalactosamine) glutamate ester 1 by the simple mixing of it with an appropriate amount 2 or b amphiphilic carboxylic acid. In 1:1 mixture (1:2), swelled under neutral pH conditions, but shrank to almost half its original volume acidic conditions. The structure and response mixed were characterized using X-ray diffraction (XRD), confocal laser...
Abstract A combinatorial approach for the synthesis of supramolecular gelators as new organic materials is described herein. In course development a convenient and flexible solid‐phase artificial glycolipids, some these compounds were accidentally found to act low molecular‐weight toward solvents. Using this glycosylated amino acetates, screening optimization organo/hydro‐gelators efficiently carried out. We that an N ‐acetyl‐galactosamine‐appended acid ester (GalNAc‐aa) gelates broad...
TRPM2 is a Ca(2+)-permeable member of the transient receptor potential melastatin family cation channels whose activation by reactive oxygen/nitrogen species (ROS/RNS) and ADP-ribose (ADPR) linked to cell death. While these are broadly expressed in CNS, presence neurons remains controversial more specifically, whether they hippocampus an open question. With this mind, we examined functional neuronal population. Using combination molecular biochemical approaches, demonstrated expression...
Catalysts hold promise as tools for chemical protein modification. However, the application of catalysts or catalyst-mediated reactions to proteins has only recently begun be addressed, mainly in vitro systems. By radically improving affinity-guided DMAP (4-dimethylaminopyridine) (AGD) that we previously reported (Koshi, Y.; Nakata, E.; Miyagawa, M.; Tsukiji, S.; Ogawa, T.; Hamachi, I. J. Am. Chem. Soc. 2008, 130, 245.), here have developed a new organocatalyst-based approach allows specific...
The functions of membrane proteins are tightly controlled by the dynamics such as protein trafficking and degradation. We demonstrated that ligand-directed acyl imidazole (LDAI) chemistry is broadly applicable to selective chemical labeling various types membrane-bound under live cell conditions without a need for any tag fragments. LDAI enabled pulse-chase analysis these determine half-life, well their degradation pathways imaging, almost natural cellular conditions.