A5068732831- Protein Tyrosine Phosphatases
- Protein Kinase Regulation and GTPase Signaling
- Pulmonary Hypertension Research and Treatments
- Cardiac Valve Diseases and Treatments
- RNA modifications and cancer
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Radical Photochemical Reactions
- Cardiac, Anesthesia and Surgical Outcomes
- Endoplasmic Reticulum Stress and Disease
- Nitric Oxide and Endothelin Effects
- Cardiomyopathy and Myosin Studies
- Anesthesia and Pain Management
- Cardiac Imaging and Diagnostics
- Coronary Interventions and Diagnostics
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Atmospheric chemistry and aerosols
- Air Quality and Health Impacts
- Traumatic Brain Injury and Neurovascular Disturbances
- Infrastructure Maintenance and Monitoring
- Acute Myocardial Infarction Research
- Aortic Disease and Treatment Approaches
- Cardiac and Coronary Surgery Techniques
- Air Quality Monitoring and Forecasting
Yokohama City University
2013-2025
The University of Tokyo
1999-2024
King's College London
2024
National Institute of Diabetes and Digestive and Kidney Diseases
2024
ProQuest (United States)
2024
Hamamatsu University School of Medicine
2024
Horiba (Japan)
2007-2023
Gifu University
2015-2023
Osaka University
2022-2023
Nagoya City University
2023
AbstractTRPA1 is a member of the transient receptor potential (TRP) cation channel family, and predominantly expressed in nociceptive neurons dorsal root ganglia (DRG) trigeminal ganglia. Activation TRPA1 by environmental irritants such as mustard oil, allicin, acrolein causes acute pain. However, endogenous ligands that directly activate remain elusive inflammation. Here, we show variety inflammatory mediators (15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), nitric oxide (NO), hydrogen...
Ca 2+ /calmodulin (CaM)-dependent phosphorylation of myosin regulatory light chain (RLC) by kinase (MLCK) initiates smooth muscle contraction and regulates actomyosin-based cytoskeletal functions in nonmuscle cells. The net extent RLC is controlled MLCK activity relative to phosphatase activity. We have constructed a CaM-sensor where -dependent CaM binding increases the catalytic domain, whereas coincident biosensor domain decreases fluorescence resonance energy transfer between two...
Ca 2+ /calmodulin (CaM)-dependent phosphorylation of myosin regulatory light chain (RLC) in smooth muscle by kinase (MLCK) and dephosphorylation phosphatase (MLCP) are subject to modulatory cascades that influence the sensitivity RLC hence contraction intracellular concentration ([Ca ] i ). We designed a CaM-sensor MLCK containing fused two fluorescent proteins linked CaM-binding sequence measure activation vivo expressed it specifically mouse muscle. In phasic bladder muscle, there was...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTConstruction of Chiral Quaternary Carbon Centers by Catalytic Enantioselective Radical-Mediated Allylation α-Iodolactones Using Allyltributyltin in the Presence a Lewis AcidMasatoshi Murakata, Toshiaki Jono, Yusuke Mizuno, and Osamu HoshinoView Author Information Faculty Pharmaceutical Sciences Science University Tokyo Shinjuku-ku, 162, Japan Cite this: J. Am. Chem. Soc. 1997, 119, 48, 11713–11714Publication Date (Web):December 3,...
Flubendiamide is a benzenedicarboxamide derivative that shows selective insecticidal activity against lepidopterous insects. The specific modulatory effects of flubendiamide on ryanodine binding in insect muscle microsomal membranes suggest the receptor (RyR) Ca(2+) release channel primary target flubendiamide. However, molecular mechanisms underlying species-specific action are unclear. We have cloned cDNA encoding novel RyR from silkworm (sRyR) and tested sensitivity to recombinant sRyR...
The reaction of a N-acyliminium ion pool with benzylsilane proceeds by chain mechanism involving oxidative C−Si bond cleavage. A radical/cation/radical cation crossover single electron transfer has been proposed. effective binary system consisting stoichiometric amount and catalytic more easily oxidized benzylstannane developed.
Background: Postoperative complication rates of carotid endarterectomy (CEA) and artery stenting (CAS) for stenosis are recommended to be maintained below a certain threshold. While the association between hospital volume complications has been reported, situation is uncertain in Japan, one countries with highest density neuro-specialists. This study aimed investigate outcomes patients who underwent CEA or CAS Japan. Methods: We retrospectively analyzed data from Japanese nationwide...
Disruption of protein synthesis and folding results in ER stress, which is associated with the pathophysiology diverse diseases affecting secretory muscle cells. Cells are protected against stress by activation unfolded response (UPR) that regulated kinase PERK, phosphorylates translation initiation factor 2 eIF2alpha to attenuate synthesis. PERK-/- cells unable modulate load experience high levels stress. In addition its role synthesis, also orchestrates many signaling events essential for...
Excitation-contraction (E-C) coupling and Ca2+-induced Ca2+ release in smooth cardiac muscles is mediated by the L-type channel isoform Cav1.2 ryanodine receptor RyR2. Although physical between Cav1.1 RyR1 skeletal muscle well established, it generally assumed that RyR2 do not directly communicate either passively or dynamically during E-C coupling. In present work, we re-examined this assumption studying detrusor of wild type Homer1-/- mice demonstrating a Homer1-mediated dynamic...
Each of the two isozymes, which are different in thermostability and quaternary structure, isocitrate dehydrogenase (NADP+) [IDH: EC 1.1.1.42] was purified to an electrophoretically homogeneous state from obligately psychrophilic marine bacterium, Vibrio sp. strain ABE-1. Hydrophobic chromatography efficient procedure separate isozymes each other. The isoelectric points isozyme I (IDH-I; a dimer, Mr 88,100) II (IDH-II; monomer, 80,500) were found be pH 4.9 5.2, respectively. similar amino...
Bilateral quadratus lumborum blockade (QLB) using ultrasound guidance has been introduced as an abdominal truncal block to improve postoperative analgesia and quality of recovery (QoR) after surgery, but efficacy remains controversial. The primary aim this study was evaluate the posterior QLB on QoR, secondarily pain gynaecological laparoscopic surgery (LS). This a single-centre randomized controlled trial. group patients underwent bilateral injections with 25-30 mL 0.25% levobupivacaine...
The enzyme which catalyzes the adenosine triphosphatedependent addition of meso-,6-diaminopimelic acid to uridine diphosphate N-acetyhnuramyl-L-alanyl-D-glutamic has been purified from Bacillus cereus approximately 270-fold.The products reaction are N-acetyhnuramyl-L-alanyl-y-D-glutamyl-meso-2,6diaminopimelic acid, dlphosphate, and inorganic phosphate.The other optical isomers 2,64aminopimelic L-lysine, fail act as substrates.In Mg++ or Mn++, I(+ NH4+ is required in this reaction.The...