A5077350209- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant Reproductive Biology
- Slime Mold and Myxomycetes Research
- Protist diversity and phylogeny
- Marine and coastal plant biology
- Plant Molecular Biology Research
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Plant and animal studies
- Diatoms and Algae Research
- Plant tissue culture and regeneration
- Biocrusts and Microbial Ecology
- Mitochondrial Function and Pathology
- Marine and coastal ecosystems
- Plant Genetic and Mutation Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Seaweed-derived Bioactive Compounds
- Plant Physiology and Cultivation Studies
- Microbial Metabolic Engineering and Bioproduction
- Polar Research and Ecology
- Plant Parasitism and Resistance
- Synthetic Organic Chemistry Methods
The University of Tokyo
2015-2024
Tokyo Future University
2019-2023
Japan Science and Technology Agency
2012-2019
Wakayama Department of Agriculture, Forestry and Fisheries
2016
Tokyo University of Science
1993-2015
National Institute of Advanced Industrial Science and Technology
2010
University of Tsukuba
2008-2010
Ube Frontier University
2001
National Institute for Basic Biology
1979-1988
Yamaguchi University
1987
The precise guidance of the pollen tube to embryo sac is critical successful sexual reproduction flowering plants. We demonstrate here in vitro by using naked Torenia fournieri, which protrudes from micropyle ovule. developed a medium for culture both ovule and T. fournieri cocultivated them thin layer solid medium. Although tubes that had germinated passed sacs, some grew semi-in through cut style arrived precisely at site entry into sac, namely, filiform apparatus synergids. When were...
Haematococcus pluvialis is a freshwater species of green algae and well known for its accumulation the strong antioxidant astaxanthin, which used in aquaculture, various pharmaceuticals, cosmetics. High levels astaxanthin are present cysts, rapidly accumulate when environmental conditions become unfavorable normal cell growth. It not understood, however, how high soluble oil, becomes possible during encystment. Here, we performed ultrastructural 3D reconstruction based on over 350 serial...
The number, size, shape and distribution of chloroplast nuclei (ct-nuclei) in variety plants, 69 species, their changes during development division, were studied with 4′6-diamidino-2-phenylindole (DAPI) epifluorescent microscopy. proplastid dormant seeds Brassica juncea contained only one ovoid ct-nucleus. This ct-nucleus increased 2- to 4-fold size dark culture became cup-shaped. Occasionally the was located near starch grains or a prolamellar body. During greening divided into about 20...
Axillary shoot formation is a key determinant of plant architecture. Formation the axillary regulated by initiation meristem or outgrowth bud. Here, we show that rice (Oryza sativa) TILLERS ABSENT1 (TAB1; also known as Os WUS), an ortholog Arabidopsis thaliana WUS, required to initiate development. We found in proceeds via transient state, which term premeristem, characterized expression OSH1, marker indeterminate cells apical meristem. In tab1-1 (wus-1) mutant, however, arrested at various...
Photosynthetic carbon partitioning into starch and neutral lipids, as well the influence of nutrient depletion replenishment on growth, pigments storage compounds, were studied in microalga, Parachlorella kessleri. Starch was utilized a primary energy compound, but drove microalgae to channel fixed lipids secondary compounds. Nutrient inhibited both cellular division growth caused degradation chlorophyll. content decreased from an initial value 25, around 10% dry weight (DW), while increased...
The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form starch rather than lipids. If complete medium, cultures grew rapidly, producing large amounts biomass a relatively short time. cells, however, contained negligible lipid reserves (1-10% DW). Treatments inducing hyperproduction lipids P. kessleri were described. absence or fivefold decreased concentration either nitrogen phosphorus sulfur. Limitation all...
ABSTRACT Mitochondrial nucleoids (mt-nucleoids) of the yeast, Saccharomyces cerevisiae, were isolated from spheroplasts stationary phase cells and their structure organization investigated by fluorescence microscopy, electron biochemical techniques. Isolated mt-nucleoids spherical or ovoid 0·3–0·6 μm in diameter, about same size shape as those observed cell DAPI staining technique. Measurement DNA content mt-nucleoids, using a video-intensified microscope system, after revealed that...
Chloroplast Division Machinery The machinery for photosynthesis, which captures the Sun's energy to generate carbohydrates, generally resides in subcellular chloroplasts of plant cells. Chloroplasts must divide as cell divides, but do so requires their own plastid dividing machinery. Yoshida et al. (p. 949 : see cover) have now analyzed single-celled alga Cyanidioschyzon merolae , whose cells each contain a single chloroplast. is made up polysaccharide chains and proteins that make them,...
The unicellular green alga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin to protect against environmental stresses. cells that accumulate in central part (green-red cyst cells) respond rapidly intense light by distributing diffusively peripheral cell within 10 min after irradiation. This response is reversible: when astaxanthin-diffused were placed dark, was redistributed center cell. Although possesses several pigments other astaxanthin, subcellular...
Abstract Sex chromosomes are particularly interesting regions of the genome for both molecular genetics and evolutionary studies; yet, most species, we lack basic information, such as gene order along chromosome. Because they recombination, Y-linked genes cannot be mapped genetically, leaving physical mapping only option establishing extent synteny homology with X Here, developed a novel general method deletion non-recombining by solving “the travelling salesman problem” evaluate its...
Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, starch productivity levels. However, further biotechnological exploitation has been hampered by lack genomic information.Here, we sequenced whole genome transcriptome, analyzed behavior P. kessleri NIES-2152 under lipid production-inducing...
Phosphorus is an essential element for life on earth and also important modern agriculture, which dependent inorganic fertilizers from phosphate rock. Polyphosphate a biological polymer of residues, accumulated in organisms during the wastewater treatment process to enhance phosphorus removal. Here, we investigated relationship between polyphosphate accumulation electron-dense bodies green alga Parachlorella kessleri. Under sulfur-depleted conditions, some symporter genes were upregulated,...
In cultured Bright Yellow-2 (BY-2) tobacco (Nicotiana tabacum) cells, the depletion of auxin (2,4-dichlorophenoxyacetic acid) in culture medium induces accumulation starch. This is accelerated by addition cytokinin (benzyladenine). Light and electron microscopic observations revealed that this amyloplast formation involves drastic changes plastid morphology. The effects on development were investigated adding or to cells grown a hormone-free culture. Auxin repressed development, whereas...
We have developed a method of isolating morphologically intact proplastid-nuclei (nucleoids) in large quantities from Nicotiana tabacum cultured cells (line BY-2) without contamination by mitochondria and cell-nuclei. Fluorescence microscopy using 4′,6-diamidino-2-phenylindole (DAPI) revealed that the compact structure isolated (pp-nuclei) was disorganized DNase I, micrococcal nuclease, proteinase K, 2 m NaCl KC1, but not affected RNase A, suggesting pp-nuclei are compactly organized an...
Abstract How do separate sexes originate and evolve? Plants provide many opportunities to address this question as they have diverse mating systems (dioecy) that evolved times independently. The classic “two-factor” model for evolution of proposes males females can evolve from hermaphrodites via the spread male female sterility mutations turn into males, respectively. This widely accepted was inspired by early genetic work in dioecious white campion (Silene latifolia) revealed presence two...
Abstract Background Previously, we isolated a mutant of Parachlorella kessleri named strain PK4 that accumulated higher concentrations lipids than the wild-type strain. Resequencing genome identified mutations in three genes which may be associated with high-lipid phenotype. The first gene, CDMT1 , encodes protein calcium-dependent membrane association domain; second DMAN1 endo-1,4-β-mannanase, while third AATPL1 plastidic ATP/ADP antiporter-like protein. Results To determine these are...