A5017981514- Shape Memory Alloy Transformations
- Oral microbiology and periodontitis research
- Titanium Alloys Microstructure and Properties
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Periodontal Regeneration and Treatments
- Surface Treatment and Residual Stress
- Oral and gingival health research
- Laser and Thermal Forming Techniques
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Algal biology and biofuel production
- Oral Health Pathology and Treatment
- HIV/AIDS oral health manifestations
- Exercise and Physiological Responses
- Luminescence Properties of Advanced Materials
- Polymer composites and self-healing
- Advanced X-ray Imaging Techniques
- X-ray Spectroscopy and Fluorescence Analysis
- Atomic and Molecular Physics
- High Altitude and Hypoxia
- Lattice Boltzmann Simulation Studies
- Metabolism, Diabetes, and Cancer
- Nuclear Physics and Applications
- Mesenchymal stem cell research
Tokyo Medical and Dental University
1971-2024
The University of Tokyo
2015-2024
Osaka University
2008-2024
Kawasaki Medical School
2023
Ludwig-Maximilians-Universität München
2023
Singapore Management University
2023
University of Tsukuba
2011-2023
Aichi Institute of Technology
2013-2023
Meiji University
2021-2023
National University of Singapore
2023
Delayed gingival wound healing is widely observed in periodontal patients with diabetes. However, the molecular mechanisms of impaired function fibroblasts diabetes remain unclear. The purpose this study was to investigate changes properties human (HGFs) under high-glucose conditions. Primary HGFs were isolated from healthy gingiva and cultured 5.5, 25, 50, 75 mM glucose for 72 h. In vitro healing, 5-ethynyl-2′-deoxyuridine (EdU), water-soluble tetrazolium salt (WST-8) assays performed...
Oxidative stress and mitochondrial dysfunction are associated with the aging process. However, role of nuclear factor erythroid 2 -related (Nrf2) in skeletal muscle during remains to be clarified. In current study, we assessed whether lack Nrf2, which is known as a master regulator redox homeostasis, promotes age-related atrophy muscle. Here, demonstrated that 4-hydroxynonenal protein carbonyls, markers oxidative stress, were robustly elevated aged Nrf2 knockout (KO) mice because decreased...
During high-intensity exercise, the concentration of ammonia is augmented in skeletal muscle. Ammonia activates phosphofructokinase and prevents oxidation pyruvate to acetyl CoA, thus leading exhaustion. Citrulline an amino acid component urea cycle liver, along with ornithine arginine. The aim this study was examine effect citrulline supplementation on fatigue performance during exercise. We constructed a swimming exercise protocol, which mice were subjected exhaustive load 5% body weight,...
The aim of this study is to investigate the mechanisms linking high glucose gingival wound healing. Bilateral wounds were created in palatal gingiva adjacent maxillary molars control rats and with streptozotocin-induced diabetes. After evaluating postsurgical closure by digital imaging, maxillae including resected for histological examinations. mRNA expressions angiogenesis, inflammation, oxidative stress markers surgical sites quantified real-time polymerase chain reaction. Primary...
To examine the potential role of lactate as a signalling molecule in skeletal muscle, we performed global gene expression analysis mouse gastrocnemius 3 h after administration using Affymetrix GeneChip system (Affymetrix, Santa Clara, Calif., USA). Among top 15 genes with largest fold change, increased Ppargc1a, Pdk4, and Ucp3 was confirmed real-time quantitative polymerase chain reaction. Our findings suggest that serves signal for upregulating related to mitochondrial function.
Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, starch productivity levels. However, further biotechnological exploitation has been hampered by lack genomic information.Here, we sequenced whole genome transcriptome, analyzed behavior P. kessleri NIES-2152 under lipid production-inducing...
Abstract Aim This study aimed to investigate the effects of diabetes care on periodontal inflammation. Materials and Methods prospective cohort included 51 Japanese patients with type 2 who underwent intensive including educational hospitalization regular outpatient treatment for 6 months. Dental prophylaxis without subgingival scaling was provided three times during observational period. Associations between changes in parameters glycaemic control levels were evaluated using multiple...
Mammalian target of rapamycin (mTOR) pathway positively regulates the cell growth through ribosome biogenesis in many type. In general, myostatin is understood to repress skeletal muscle hypertrophy inhibition mTOR and myogenesis. However, these relationships have not been clarified undergoing atrophy. Here, we observed a significant decrease mass at 2 weeks after denervation. Unexpectedly, however, expression genes related myogenesis were markedly increased, that was decreased. de novo...
It is known that repeated bouts of high-intensity interval training (HIIT) lead to enhanced levels glycolysis, glycogenesis, and lactate transport proteins in skeletal muscle; however, little about the molecular mechanisms underlying these adaptations. To decipher mechanism leading improvement muscle glycolytic capacity associated with HIIT, we examined role hypoxia-inducible factor-1α (Hif-1α), major transcription factor regulating expression genes related anaerobic metabolism, adaptation...
Few studies have reported on the impact of oxidative stress dental implant failure. The aim this study was to investigate hyperglycemia-induced osseointegration in diabetes mellitus (DM).Acid-treated titanium implants were bilaterally placed maxillary alveolar ridge streptozotocin-induced diabetic (DM group) and control rats after extraction first molars. Histological analysis micro-push-out test performed 4 weeks surgery. Oxidative osteogenic markers surrounding bone quantified by real-time...
<title>Abstract</title> Objectives Obesity is a risk factor for periodontal disease and associated with socioeconomic status (SES). However, it remains unclear whether SES modifies the relationship between obesity disease. This study aimed to investigate influence of on association Material Methods We used multilevel Poisson regression, adjusted SES, analyze body mass index (BMI) parameters 962 participants (mean age 58.3 years; SD: 13.8). was assessed based average income education levels...
To examine the applicability of hematite ore to ironmaking by microwave heating, carbothermic reduction powders heating were elucidated using gas analysis exhausted gas, and compared with that magnetite powders. The temperature – graphite mixed moderately increases at beginning, then, starts increase abruptly around 400°C owing thermal runaway Fe2O3. Once reaction take place, rate is even faster than magnetite, which same behavior as observed conventional heating. Hematite could be a...
Abstract Oxidative stress‐induced mitochondrial dysfunction is associated with age‐related and disuse‐induced skeletal muscle atrophy. However, the role of nuclear factor erythroid 2‐related 2 (Nrf2) during fiber atrophy remains to be elucidated. In this study, we examined whether deficiency Nrf2, a master regulator antioxidant transcription, promotes denervation‐induced fragmentation We found that expression Nrf2 its target genes was upregulated at weeks after denervation in wild‐type ( WT...
Information regarding periodontal regenerative therapy in patients with diabetes mellitus (DM) is limited. This pilot study compared the outcomes of minimally invasive surgery using enamel matrix derivative (EMD) between DM and non-DM patients.This prospective included deep intrabony defects or without type 2 DM. Minimally surgical technique (MIST) modified MIST (M-MIST) EMD, bone graft materials, was performed. Periodontal examination intraoral radiography were performed at baseline, 6...
Introduction The aim was to investigate the relationship of full-mouth inflammatory parameters periodontal disease with diabetes and obesity. Research design methods This cross-sectional study conducted diabetes-related examinations calculated inflamed epithelial surface area (PISA PESA) 71 Japanese patients type 2 diabetes. Multiple linear regression analyses were performed evaluate associations between PISA or PESA obesity parameters. Results Median value body mass index (BMI), hemoglobin...
The present study aimed to investigate the periodontal regenerative effect of enamel matrix derivative (EMD) in diabetes. Thirty-six rats were assigned streptozotocin-induced diabetes or control (non-diabetic) groups. Three-wall intrabony defects surgically generated bilateral maxilla molar, followed by application EMD saline. Primary wound closure and defect fill evaluated via histomorphological analysis micro-computed tomography. mRNA expression levels inflammatory angiogenic factors...
Abstract The aim of this study was to investigate the impact oral hygiene, periodontal diseases, and dental caries on all-cause mortality in hemodialysis. This prospective cohort included 266 patients with end-stage renal disease who were undergoing Medical interviews, blood biochemical tests, comprehensive examinations including pocket examination all teeth plaque accumulation by debris index-simplified (DI-S), performed. Survival rates assessed at a 3-year follow-up. Overall, 207...
Abstract Malnutrition-inflammation-atherosclerosis (MIA) syndrome is a significant risk factor for mortality in patients undergoing hemodialysis. This study aimed to investigate the association between MIA and oral health status hemodialysis patients. A cross-sectional was conducted on 254 Comprehensive medical dental examinations were performed. Three components included define syndrome: Geriatric Nutritional Risk Index, serum high-sensitivity C-reactive protein, history of cardiovascular...
It is now evident that exercise training leads to increases in monocarboxylate transporter (MCT)1 and MCT4, but little known about the mechanisms of coupling muscle contraction with these changes. The aim this study was investigate effect 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) induced activation AMP-activated protein kinase (AMPK) on MCT1, GLUT4 denervated muscle. Protein levels MCT4 after 10 days denervation were significantly decreased mice gastrocnemius muscle,...
We used a microplate-based method to quantify microalgal lipids with Nile Red staining and fluorescence microplate reader. However, starch that combines microplates has not been reported. Therefore, we examined quantification of using Lugol staining. Neither nor accumulated during the zero phase cultured Parachlorella kessleri, only phase, was subsequently lost oil phase. The quantities were measured method, which indicated linear production within limited ranges (lipids, 0.071–0.380 g mL−1;...
The purposes of our study were to examine (1) Rh B glycoprotein (Rhbg) and C (Rhcg) expressions in mouse skeletal muscle; (2) the effect 6-week training on Rhbg Rhcg expressions. levels much higher soleus (Sol) than plantaris (Pla) or gastrocnemius (Gas). Immunofluorescence microscopy demonstrated that colocalizes with dystrophin, a plasma membrane protein marker, whereas CD31, vascular endothelial cell marker. In swim study, we set up two different groups. Endurance (END) group underwent...
Lactate is a metabolic product of glycolysis and has recently been shown to act as signaling molecule that induces adaptations in oxidative metabolism. In this study, we investigated whether lactate administration enhanced muscle hypertrophy protein synthesis responses during resistance exercise animal models. We used male ICR mice (7-8 weeks old) were for chronic (mechanical overload induced by synergist ablation: [OL]) acute (high-intensity contraction electrical stimulation: [ES]) The...