A5036460871- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Cardiovascular and exercise physiology
- Sports Performance and Training
- Pharmacological Effects and Assays
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Glycogen Storage Diseases and Myoclonus
- High Altitude and Hypoxia
- Metabolism, Diabetes, and Cancer
- Veterinary Equine Medical Research
- Autophagy in Disease and Therapy
- Genomics, phytochemicals, and oxidative stress
- Genetics and Physical Performance
- Genetic Neurodegenerative Diseases
- Muscle activation and electromyography studies
- Neuroscience and Neuropharmacology Research
- Physiological and biochemical adaptations
- Neurological disorders and treatments
- Biochemical Acid Research Studies
- Calpain Protease Function and Regulation
- PI3K/AKT/mTOR signaling in cancer
Kanagawa University
2018-2025
The University of Tokyo
2009-2022
Yong In University
2022
Meikai University
2022
McMaster University
2013-2021
University of Tsukuba
2010-2013
Institute for Environmental Sciences
2011
Japan Racing Association
2010-2011
Abstract Resistance exercise (RE) activates signalling by the mammalian target of rapamycin (mTOR) and it has been suggested that rapamycin-sensitive mTOR controls RE-induced changes in protein synthesis, ribosome biogenesis, autophagy expression peroxisome proliferator gamma coactivator 1 alpha (PGC-1α). However, direct evidence to support aforementioned relationships is lacking. Therefore, this study, we investigated role activation muscle PGC-1α hypertrophy. The results indicated...
Oxidative stress and mitochondrial dysfunction are associated with the aging process. However, role of nuclear factor erythroid 2 -related (Nrf2) in skeletal muscle during remains to be clarified. In current study, we assessed whether lack Nrf2, which is known as a master regulator redox homeostasis, promotes age-related atrophy muscle. Here, demonstrated that 4-hydroxynonenal protein carbonyls, markers oxidative stress, were robustly elevated aged Nrf2 knockout (KO) mice because decreased...
To examine the potential role of lactate as a signalling molecule in skeletal muscle, we performed global gene expression analysis mouse gastrocnemius 3 h after administration using Affymetrix GeneChip system (Affymetrix, Santa Clara, Calif., USA). Among top 15 genes with largest fold change, increased Ppargc1a, Pdk4, and Ucp3 was confirmed real-time quantitative polymerase chain reaction. Our findings suggest that serves signal for upregulating related to mitochondrial function.
Traumatic nerve injury or disease leads to denervation and severe muscle atrophy. Recent evidence shows that mitochondrial loss could be a key mediator of skeletal Here, we show daily heat stress treatment rescues denervation-induced mitochondria concomitant We also found denervation-activated autophagy-dependent clearance (mitophagy) was suppressed by treatment. The molecular basis this observation is explained our results showing attenuates the increase proteins regulate tagging step for...
Abstract Background Human genetic disorders and transgenic mouse models have shown that mitochondrial DNA (mtDNA) mutations telomere dysfunction instigate the aging process. Epidemiologically, exercise is associated with greater life expectancy reduced risk of chronic diseases. While beneficial effects are well established, molecular mechanisms instigating these observations remain unclear. Results Endurance reduces mtDNA mutation burden, alleviates multisystem pathology, increases lifespan...
It is known that repeated bouts of high-intensity interval training (HIIT) lead to enhanced levels glycolysis, glycogenesis, and lactate transport proteins in skeletal muscle; however, little about the molecular mechanisms underlying these adaptations. To decipher mechanism leading improvement muscle glycolytic capacity associated with HIIT, we examined role hypoxia-inducible factor-1α (Hif-1α), major transcription factor regulating expression genes related anaerobic metabolism, adaptation...
Abstract Electrical stimulation is widely used to investigate localised muscle adaptations, with applications in both sports and rehabilitation. However, the systemic effects of electrical stimulation, particularly contralateral muscles that are not directly stimulated, well understood. This study investigated whether unilateral induces transcriptional changes electrically stimulated (ES) non‐stimulated (non‐ES) legs, compared legs sedentary control mice. RNA‐sequence analysis revealed 1320...
High-intensity interval training (HIIT) can increase mitochondrial volume in skeletal muscle. However, it is unclear whether HIIT alters the intrinsic capacity of fatty acid oxidation, or such changes are associated with FAT/CD36, a regulator reciprocal nuclear receptor coactivator (peroxisome proliferator-activated gamma coactivator-1alpha (PGC-1α)) and corepressor (receptor-interacting protein 140 (RIP140)). We examined oxidation rates isolated subsarcolemmal (SS) intermyofibrillar (IMF)...
It is well known that resistance exercise increases muscle protein synthesis and strength. However, little about the effect of on mitochondrial dynamics, which coupled with function. In skeletal muscle, mitochondria exist as dynamic networks are continuously remodeling through fusion fission. The purpose this study was to investigate acute chronic exercise, induces hypertrophy, expression proteins related dynamics in rat muscle. Resistance consisted maximum isometric contraction, induced by...
Growing evidence shows that lactate is not merely an intermediate metabolite, but also a potential signaling molecule. However, whether daily administration induces physiological adaptations in skeletal muscle remains to be elucidated. In this study, we first investigated the effects of (equivalent 1 g/kg body weight) for 3 weeks on mitochondrial muscle. We demonstrated 3-week increased enzyme activity (citrate synthase, 3-hydroxyacyl CoA dehydrogenase, and cytochrome c oxidase) plantaris...
The aim of this study was to investigate the effects training and detraining on monocarboxylate transporter (MCT) 1 MCT4 levels in gluteus medius muscle Thoroughbred horses. Twelve horses were used for analysis. For 18 weeks, all underwent high-intensity (HIT), with running at 90-110% maximal oxygen consumption (VO2 max ) 3 min, 5 days week(-1). Thereafter, either 6 weeks by min moderate-intensity (MIT) 70% VO2 max, week(-1) (HIT-MIT group) or stall rest (HIT-SR group). an incremental...
Recent studies have demonstrated that resistance exercise leads not only to muscle hypertrophy, but it also improves mitochondrial function. Because calorie restriction (CR) has been suggested as a way induce biogenesis, we examined the effects of training with or without CR on weight and key parameters in rat skeletal muscle. Four weeks (thrice/wk) resulted increased gastrocnemius by 14% rats fed ad libitum (AL). The degree muscle-weight increase via was lower (7.4%). showed no effect...
Abstract Oxidative stress‐induced mitochondrial dysfunction is associated with age‐related and disuse‐induced skeletal muscle atrophy. However, the role of nuclear factor erythroid 2‐related 2 (Nrf2) during fiber atrophy remains to be elucidated. In this study, we examined whether deficiency Nrf2, a master regulator antioxidant transcription, promotes denervation‐induced fragmentation We found that expression Nrf2 its target genes was upregulated at weeks after denervation in wild‐type ( WT...
Nuclear factor erythroid 2-related 2 Nfe2l2 (Nrf2) is believed to play a crucial role in protecting cells against oxidative stress. In addition its primary function of maintaining redox homeostasis, there emerging evidence that Nrf2 also involved energy metabolism. this review, we briefly discuss the skeletal muscle metabolism from perspective exercise physiology. This article part special issue "Mitochondrial Function, Reactive Oxygen/Nitrogen Species and Skeletal Muscle" edited by Håkan...
It is now evident that exercise training leads to increases in monocarboxylate transporter (MCT)1 and MCT4, but little known about the mechanisms of coupling muscle contraction with these changes. The aim this study was investigate effect 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) induced activation AMP-activated protein kinase (AMPK) on MCT1, GLUT4 denervated muscle. Protein levels MCT4 after 10 days denervation were significantly decreased mice gastrocnemius muscle,...
High-intensity interval training (HIIT) consists of repeated short bursts high-intensity exercise and rest. Here we review recent work focusing on the metabolic adaptations to HIIT, especially in oxidative capacity substrate availability skeletal muscle. In this review, HIIT is defined as chronic training, for at least 2 weeks, involving short-duration >85% VO2 max, followed by complete rest or active rest, any given duration First, describe effects muscle metabolism, specifically terms...
We tested the hypothesis that oral lactate supplementation increases mitochondrial enzyme activity given potential role of for inducing biogenesis. In this study, mice were assigned to a saline-ingested sedentary group (S+S; n = 8), lactate-ingested (L+S; 9), training (S+T; and (L+T; 8). Mice in S+S S+T groups received saline, whereas L+S L+T sodium (equivalent 5 g/kg body weight) via gavage days week 4 weeks. At 30 min after ingestion, performed endurance (treadmill running, 20 m/min, min,...
This study aimed to examine the effects of voluntary wheel running on cancer cachexia-induced mitochondrial alterations in mouse skeletal muscle. Mice bearing colon 26 adenocarcinoma (C26) were used as a model cachexia. C26 mice showed lower gastrocnemius and plantaris muscle weight, but 4 weeks exercise rescued these changes. Further, attenuated observed declines levels oxidative phosphorylation proteins activities citrate synthase cytochrome c oxidase mice. Among morphology regulatory...
ABSTRACT High-intensity interval training has attracted considerable attention as a time-efficient strategy for inducing physiological adaptations, but the underlying mechanisms have yet to be elucidated. By using metabolomics techniques, we investigated changes in metabolic network responses Thoroughbred horses high-intensity exercise performed with two distinct (15 min or 2 min) rest intervals. The peak plasma lactate level was higher during duration than that 15 (24.5±6.8 versus 13.3±2.7...
We investigated whether calorie restriction (CR) enhances metabolic adaptations to endurance training (ET). Ten-week-old male Institute of Cancer Research (ICR) mice were fed ad libitum or subjected 30% CR. The subdivided into sedentary and ET groups. group performed treadmill running (20-25 m/min, 30 min, 5 days/week) for weeks. found that CR decreased glycolytic enzyme activity monocarboxylate transporter (MCT) 4 protein content, while enhancing glucose content in the plantaris soleus...
McArdle disease (glycogen storage Type V; MD) is a metabolic myopathy caused by deficiency in muscle glycogen phosphorylase. Since an important fuel for during exercise, this inborn error of metabolism provides model understanding the role function and compensatory adaptations that occur response to impaired glycogenolysis. Patients with MD have exercise intolerance symptoms including premature fatigue, myalgia, and/or cramps. Despite this, patients are able perform prolonged as result...
This study investigated whether endurance training attenuates orchiectomy (ORX)-induced metabolic alterations. At 7 days of recovery after sham operation or ORX surgery, the mice were randomized to remain sedentary undergo 5 weeks treadmill running (15-20 m/min, 60 min, days/week). decreased glycogen concentration in gastrocnemius muscle, enhanced phosphofructokinase activity plantaris and lactate dehydrogenase soleus muscles. Mitochondrial enzyme activities protein content muscles also ORX,...