A5016860157- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Photosynthetic Processes and Mechanisms
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine and coastal plant biology
- Pain Management and Opioid Use
- Helicobacter pylori-related gastroenterology studies
- Genomics and Phylogenetic Studies
- Respiratory Support and Mechanisms
- Biocrusts and Microbial Ecology
- Heavy metals in environment
- Diatoms and Algae Research
- Anesthesia and Pain Management
- Inflammatory mediators and NSAID effects
- Microbial Metabolic Engineering and Bioproduction
- Geochemistry and Elemental Analysis
- Pharmacological Effects and Toxicity Studies
- Infrastructure Maintenance and Monitoring
- Intensive Care Unit Cognitive Disorders
- Environmental Toxicology and Ecotoxicology
- Fungal Infections and Studies
- Cystic Fibrosis Research Advances
- Remote Sensing and Land Use
Japanese Red Cross Kyushu International College of Nursing
2025
The University of Tokyo
2012-2024
National Institute for Environmental Studies
2018-2024
Kanagawa Cancer Center
2014-2023
Kanagawa Prefectural Hospital Organization
2014-2023
Yao Tokushukai General Hospital
2022-2023
Kanagawa University
2021-2023
Fukuoka Tokushukai Hospital
2017-2022
Tokyo University of Agriculture and Technology
2021
Life Science Institute
2021
Haematococcus pluvialis is a freshwater species of green algae and well known for its accumulation the strong antioxidant astaxanthin, which used in aquaculture, various pharmaceuticals, cosmetics. High levels astaxanthin are present cysts, rapidly accumulate when environmental conditions become unfavorable normal cell growth. It not understood, however, how high soluble oil, becomes possible during encystment. Here, we performed ultrastructural 3D reconstruction based on over 350 serial...
The unicellular green alga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin to protect against environmental stresses. cells that accumulate in central part (green-red cyst cells) respond rapidly intense light by distributing diffusively peripheral cell within 10 min after irradiation. This response is reversible: when astaxanthin-diffused were placed dark, was redistributed center cell. Although possesses several pigments other astaxanthin, subcellular...
Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, starch productivity levels. However, further biotechnological exploitation has been hampered by lack genomic information.Here, we sequenced whole genome transcriptome, analyzed behavior P. kessleri NIES-2152 under lipid production-inducing...
Phosphorus is an essential element for life on earth and also important modern agriculture, which dependent inorganic fertilizers from phosphate rock. Polyphosphate a biological polymer of residues, accumulated in organisms during the wastewater treatment process to enhance phosphorus removal. Here, we investigated relationship between polyphosphate accumulation electron-dense bodies green alga Parachlorella kessleri. Under sulfur-depleted conditions, some symporter genes were upregulated,...
Abstract The type of Haematococcus Flot. (Volvocales, Chlorophyceae) accumulates astaxanthin in its dormant stage. Currently, only one species is recognized the genus; however, it referred to by two names: H. lacustris (Gir.‐Chantr.) Rostaf. and pluvialis This confusion arose partly because misunderstanding over nomenclature inadequate descriptions Volvox Gir.‐Chantr. . Here, we designate an epitype based on a single cryopreserved culture that supports newly designated lectotype V. Our...
Abstract Background Previously, we isolated a mutant of Parachlorella kessleri named strain PK4 that accumulated higher concentrations lipids than the wild-type strain. Resequencing genome identified mutations in three genes which may be associated with high-lipid phenotype. The first gene, CDMT1 , encodes protein calcium-dependent membrane association domain; second DMAN1 endo-1,4-β-mannanase, while third AATPL1 plastidic ATP/ADP antiporter-like protein. Results To determine these are...
The COVID-19 pandemic has affected healthcare workers and medical students including the restriction of nursing from clinical practice, which hinders learning process. This study examined changes in nurses' perceptions concerning students' practice education during pandemic.A web-based questionnaire was administered to nurses two facilities, comprised pre- post-pandemic teaching experiences. IBM SPSS Statistics for Windows, Version 26.0 (Released 2019; Corp., Armonk, NY, USA) KH Coder (KH...
Although the clinical efficacy of prostaglandins (PGs), especially on gastric mucosal injuries induced by nonsteroidal antiinflammatory drugs, is widely appreciated, their mechanism action, apart from acid suppression, quite unclear. In this study, we have established a primary culture system human fibroblasts and clearly demonstrated that PGs strongly induce expression hepatocyte growth factor (HGF) in fibroblasts, which mediated PGE specific receptor, EP2 or EP4. Since HGF facilitates...
Aims Glypican 3 (GPC3) is a cell surface heparan sulphate proteoglycan expressed specifically in the fetal liver and malignant neoplasms of hepatocyte lineage. The aim was to evaluate significance GPC3 α‐fetoprotein (AFP)‐producing gastric carcinoma (GC) other forms GC. Methods results We immunohistochemically evaluated expression representative cases AFP‐producing GC tissue microarray consecutive series GCs with markers lineage (AFP, PIVKA‐II antigen, HEP). In 10 GC, we observed...
Cellular bioenergetic failure caused by mitochondrial dysfunction is a key process of alveolar epithelial injury during acute respiratory distress syndrome (ARDS). Prolyl hydroxylases (PHDs) act as cellular oxygen sensors, and their inhibition activates hypoxia-inducible factor (HIF), resulting in enhanced glycolytic activity, which could compensate for impaired function protect cells from ARDS. Here, we evaluated the effects pharmacological PHD with dimethyloxalylglycine (DMOG) on cell...
Patients with acute respiratory distress syndrome receiving mechanical ventilation show inhomogeneous lung aeration. Atelectasis during uneven leads to alveolar hypoxia and could therefore result in inflammation injury. We aimed elucidate whether how atelectasis causes hypoxia-induced an open-chest differential-ventilation rat model.We first investigated inflammatory histological changes the bilateral lungs of unilaterally ventilated rats, which right was atelectatic left high tidal volume...
A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties producing lipids extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount acidic EPS, when cultured in nitrogen-deficient BG110 medium (BG11–N) with 2 % CO2-air supply. main component intracellularly accumulated triacylglycerol (TAG), depending on the different cultivation conditions BG11, BG11–N, BG11–P (phosphate depleted), BG11–N–P (nitrogen phosphate...
Summary Septins are a group of GTP ‐binding proteins that multi‐functional, with well‐known role in cytokinesis animals and fungi. Although the functions septins have been thoroughly studied opisthokonts (fungi animals), function evolution plant/algal not as well characterized. Here we describe septin localization expression green algae N annochloris bacillaris M arvania geminata . The present data suggest localize at division site when occurs. In addition, show homologs may be found only...
We used a microplate-based method to quantify microalgal lipids with Nile Red staining and fluorescence microplate reader. However, starch that combines microplates has not been reported. Therefore, we examined quantification of using Lugol staining. Neither nor accumulated during the zero phase cultured Parachlorella kessleri, only phase, was subsequently lost oil phase. The quantities were measured method, which indicated linear production within limited ranges (lipids, 0.071–0.380 g mL−1;...
Secondary plastids are acquired by the engulfment and retention of eukaryotic algae, which results in an additional surrounding membrane or pair membranes relative to more familiar primary land plants. In most cases, endocytosed alga loses its genome as it becomes integrated, but two algal groups, cryptophytes chlorarachniophytes, secondary retain a vestigial nucleus periplastidal compartment (PPC), remnant cytoplasm between inner outer pairs. Many essential housekeeping genes missing from...
A new species of a chlorarachniophyte alga, Bigelowiella longifila sp. nov., is described. It classified as member flagellate cells constitute the main stage life cycle. However, this alga different from only described genus, B. natans Moestrup, in having unique amoeboid We observed an interesting behavior daughter after cell division: One two inherits long filopodium parental cell, and it subsequently transports its contents through to develop at opposite end. The other forms filopodium....