A5057138397- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Environmental DNA in Biodiversity Studies
- Marine Biology and Ecology Research
- Genomics and Phylogenetic Studies
- Arctic and Antarctic ice dynamics
- Cystic Fibrosis Research Advances
- Food Industry and Aquatic Biology
- Antibiotic Resistance in Bacteria
- Photosynthetic Processes and Mechanisms
- Mycobacterium research and diagnosis
- Otolaryngology and Infectious Diseases
- Infectious Diseases and Tuberculosis
- Arctic and Russian Policy Studies
- Methane Hydrates and Related Phenomena
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Marine and coastal plant biology
- Tuberculosis Research and Epidemiology
- Healthcare Decision-Making and Restraints
- Wastewater Treatment and Nitrogen Removal
- Nosocomial Infections in ICU
- Ocean Acidification Effects and Responses
- Bacillus and Francisella bacterial research
Station Biologique de Roscoff
2008-2024
Adaptation et Diversité en Milieu Marin
2011-2024
Centre National de la Recherche Scientifique
2008-2023
Centre Hospitalier de Cornouaille
2010-2023
Hospices Civils de Lyon
2023
Université Claude Bernard Lyon 1
2023
Centre International de Recherche en Infectiologie
2023
Inserm
2023
École Normale Supérieure de Lyon
2023
Sorbonne Université
2004-2022
Prochlorococcus marinus , the dominant photosynthetic organism in ocean, is found two main ecological forms: high-light-adapted genotypes upper part of water column and low-light-adapted at bottom illuminated layer. P. SS120, complete genome sequence reported here, an extremely form. The SS120 composed a single circular chromosome 1,751,080 bp with average G+C content 36.4%. It contains 1,884 predicted protein-coding genes size 825 bp, rRNA operon, 40 tRNA genes. Together 1.66-Mbp MED4, one...
Summary The importance of the cyanobacteria Prochlorococcus and Synechococcus in marine ecosystems terms abundance primary production can be partially explained by ecotypic differentiation. Despite dominance eukaryotes within photosynthetic picoplankton many areas a similar differentiation has never been evidenced for these organisms. Here we report distinct genetic [rDNA 18S internal transcribed spacer (ITS) sequencing], karyotypic (pulsed‐field gel electrophoresis), phenotypic (pigment...
Abstract Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of biosphere. The ongoing environmental metabarcoding revolution opens perspective for holistic biological studies these organisms, particular unicellular microalgae that often lack distinctive morphological characters and complex life cycles. To interpret sequences, necessarily relies on taxonomically curated databases containing reference sequences targeted gene (or barcode) from identified...
DNA barcoding offers an efficient way to determine species identification and measure biodiversity. For dinoflagellates, ancient alveolate group of about 2000 described extant species, studies have revealed large amounts unrecognized diversity, most which is not represented in culture collections. To date, two mitochondrial gene markers, Cytochrome Oxidase I (COI) b oxidase (COB), been used assess both failed amplify all taxa suffered from low resolution. Nevertheless, genes yielded many...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 43:79-93 (2006) - doi:10.3354/ame043079 Analysis of photosynthetic picoeukaryote diversity at open ocean sites in Arabian Sea using a PCR biased towards marine algal plastids Nicholas J. Fuller1, Colin Campbell1, David Allen1, Frances D. Pitt1, Katrin Zwirglmaier1, Florence Le Gall2, Daniel Vaulot2, Scanlan1,*...
Pigment analysis performed on 30 Prasinophyceae strains revealed two main groups: the prasinoxanthin‐containing and prasinoxanthin‐less Prasinophyceae. Prasinoxanthin‐containing comprised orders Mamiellales, Pseudoscourfieldiales ( Pycnococcaceae ), Prasinococcales. For this group, classification with pigment composition showed a good agreement molecular phylogeny. except Crustomastix stigmatica , accumulated uriolide, micromonal, dihydrolutein, Unidentified M1 as characteristic pigments....
Recent molecular investigations of marine samples taken from different environments, including tropical, temperate and polar areas, as well deep thermal vents, have revealed an unexpectedly high diversity protists, some them forming deep-branching clades within important lineages, such the alveolates heterokonts. Using same approach on coastal samples, we identified a novel group protist small subunit (SSU) rDNA sequences that do not correspond to any phylogenetic previously identified....
ABSTRACT Cystic fibrosis (CF) is the most frequent lethal genetic disease in Caucasian population. Lung destruction principal cause of death by chronic Pseudomonas aeruginosa colonization. There a high prevalence oropharyngeal anaerobic bacteria sputum CF patients. This study was carried out due to lack results comparing subgingival periodontal pathogenic between oral cavity and lungs patients with relation P. presence. Our first goal detect samples culture molecular methods determine...
Abstract. The Green Edge initiative was developed to investigate the processes controlling primary productivity and fate of organic matter produced during Arctic phytoplankton spring bloom (PSB) determine its role in ecosystem. Two field campaigns were conducted 2015 2016 at an ice camp located on landfast sea southeast Qikiqtarjuaq Island Baffin Bay (67.4797∘ N, 63.7895∘ W). During both expeditions, a large suite physical, chemical biological variables measured beneath consolidated sea-ice...
Abstract Major seasonal community reorganizations and associated biomass variations are landmarks of plankton ecology. However, the processes turnover rates have not been fully elucidated so far. Here, we analyse patterns planktonic protist succession in temperate latitudes, based on quantitative taxonomic data from both microscopy counts (cells >10 μm) ribosomal DNA metabarcoding (size fraction >3 μm, 18S rRNA gene) samples collected bimonthly over 8 years (2009–2016) at SOMLIT‐Astan...
Prochlorococcus is an important component of phototrophic biomass in oligotrophic areas. In such systems, diel variations optical properties have been reported. order to better understand these natural variations, the (absorption, attenuation, and scattering) biochemical (carbon pigment) axenic clone , PCC 9511, grown a turbidostat set under light conditions that simulated those found near ocean surface, were monitored every 2 h for several consecutive days. All parameters showed pronounced...
The marine diatom Guinardia delicatula is a cosmopolitan species that dominates seasonal blooms in the English Channel and North Sea. Several eukaryotic parasites are known to induce mortality of this species. Here, we report isolation characterization first viruses infect G. delicatula. Viruses were isolated from Western (SOMLIT-Astan station) during late summer bloom decline A combination laboratory approaches revealed these lytic (GdelRNAV) small tailless particles 35-38 nm diameter...
We developed oligonucleotide probes, based on plastid 16S ribosomal DNA (rDNA) sequences, to target 10 different algal classes (Chlorarachniophyceae, Chrysophyceae, Cryptophyceae, Eustigmatophyceae, Pavlovophyceae, Pelagophyceae, Pinguiophyceae, Prasinophyceae [clade VI], Prymnesiophyceae, and Trebouxiophyceae), for use with dot blot hybridization technology. These class‐specific probes were then used investigate the community structure of photosynthetic picoeukaryotes (≪3 µm) along a...
Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under pack ice. An extensive culturing effort was conducted before and during a bloom in Baffin Bay between April July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single pipetting, serial dilution. Although all three techniques yielded most common organisms, each technique retrieved specific taxa, highlighting importance of using several methods to...
Abstract. In late 2004, the BIOSOPE cruise sailed between equatorial influenced waters off Marquesas islands and nutrient enriched of Chilean upwelling. Along way, it explored Southeast Pacific gyre centred around Easter Island, which is probably most oligotrophic oceanic region on earth. During this cruise, we undertook a vigorous effort to isolate novel photosynthetic picoplanktonic eukaryotes. Two strategies were attempted board: enrichment samples with culture medium sorting specific...
Abstract Background The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa . This bacterium plays an important role in the poor outcome CF patients. During disease progress, first acquisition P. key-step management Quantitative PCR (qPCR) offers opportunity detect earlier by Given lack a validated protocol, our goal was find optimal molecular protocol for detection Methods We compared two formerly described qPCR formats early sputum samples:...
Limited pharmacokinetics data support dalbavancin long-term use in off-label indications and the optimal dosing regimen is debated. We aimed to describe concentrations an observational retrospective multicentre study.
Abstract. Aerobic anoxygenic phototrophic (AAP) bacteria play significant roles in the bacterioplankton productivity and biogeochemical cycles of surface ocean. In this study, we applied both cultivation mRNA-based molecular methods to explore diversity AAP along an oligotrophic gradient Mediterranean Sea early summer 2008. Colony-forming units obtained on three different agar media were screened for production bacteriochlorophyll-a (BChl-a), light-harvesting pigment bacteria....