A5028967936- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Environmental DNA in Biodiversity Studies
- Marine Biology and Ecology Research
- Arctic and Antarctic ice dynamics
- Methane Hydrates and Related Phenomena
- Food Industry and Aquatic Biology
- Arctic and Russian Policy Studies
- Oceanographic and Atmospheric Processes
- Marine Sponges and Natural Products
- Coral and Marine Ecosystems Studies
- Coastal wetland ecosystem dynamics
- Ocean Acidification Effects and Responses
- Parasite Biology and Host Interactions
- Geological and Geophysical Studies
- Polar Research and Ecology
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Marine Bivalve and Aquaculture Studies
Universidad Mayor
2019-2024
Station Biologique de Roscoff
2018-2022
Centre National de la Recherche Scientifique
2018-2022
Sorbonne Université
2018-2022
Marine Ecology Research Institute
2020
Adaptation et Diversité en Milieu Marin
2020
Universidade de São Paulo
2013-2018
Universidade Federal do Paraná
2011
For almost three decades, flow cytometry has allowed researchers to investigate ocean planktonic communities using size and cell fluorescence properties. However, oceanographic applications must face two constraints. First, when dealing with marine microbes, instruments be sensitive because these organisms are very small low fluorescence. Second, portable used on board ships. We compared the performance of instruments, BD FACSCanto™ Accuri™ C6. The former is an expensive laboratory-based...
Abstract. The Green Edge initiative was developed to investigate the processes controlling primary productivity and fate of organic matter produced during Arctic phytoplankton spring bloom (PSB) determine its role in ecosystem. Two field campaigns were conducted 2015 2016 at an ice camp located on landfast sea southeast Qikiqtarjuaq Island Baffin Bay (67.4797∘ N, 63.7895∘ W). During both expeditions, a large suite physical, chemical biological variables measured beneath consolidated sea-ice...
Abstract Marine sponges host diverse microbial communities. Although we know many of its ecological patterns, a deeper understanding the polar sponge holobiont is still needed. We combine high-throughput sequencing ribosomal genes, including largest taxonomic repertoire Antarctic species analyzed to date, functional metagenomics, and metagenome-assembled genomes (MAGs). Our findings show that harbor more exclusive bacterial archaeal communities than seawater, while eukaryotes are mostly...
Pico and nanoplankton communities from the Southwest Atlantic Ocean along Brazilian Bight are poorly described. The hydrography in this region is dominated by a complex system of layered water masses, which includes warm oligotrophic Tropical Water (TW), cold nutrient rich South Central (SACW) Coastal (CW), have highly variable properties. In order to assess how pico- distributed these different we determined flow cytometry abundance heterotrophic bacteria, Prochlorococcus, Synechococcus...
Abstract Year-round reports of phytoplankton dynamics in the West Antarctic Peninsula are rare and mainly limited to microscopy and/or pigment-based studies. We analyzed community from coastal waters Fildes Bay between January 2014 2015 using metabarcoding nuclear plastidial 18/16S rRNA gene both size-fractionated flow cytometry sorted samples. Overall 14 classes photosynthetic eukaryotes were present our samples with following dominating: Bacillariophyta (diatoms), Pelagophyceae...
Seasonal phytoplankton blooms are important Arctic phenomena, contributing to global primary production and biogeochemical cycling. The decline in sea-ice extent thickness favors a longer open-water period with impacts on dynamics. net productivity is influenced by microalgae living associated sea ice, distinct species thought be favored ice-covered ice-free waters. In this study, we investigated the community structure Baffin Bay, semi-enclosed where North Atlantic water masses interact. We...
Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under pack ice. An extensive culturing effort was conducted before and during a bloom in Baffin Bay between April July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single pipetting, serial dilution. Although all three techniques yielded most common organisms, each technique retrieved specific taxa, highlighting importance of using several methods to...
Members of the class Mamiellophyceae comprise species that can dominate picophytoplankton diversity in polar waters. Yet, are often morphologically indistinguishable from temperate species, although clearly separated by molecular features. Here we examine four strains Canadian Arctic. The 18S rRNA and Internal Transcribed Spacer 2 (ITS2) gene phylogeny place these within family Mamiellaceae (Mamiellales, Mamiellophyceae) two separate clades genus Mantoniella. ITS2 synapomorphies support...
Photosynthetic microbial eukaryotes play a pivotal role as primary producers in the Arctic Ocean, where seasonal blooms within and below ice are crucial phenomena, contributing significantly to global production biogeochemical cycling. In this study, we investigated taxonomic composition of sympagic algae phytoplankton communities during under-ice spring bloom using metabarcoding 18S rRNA gene. Samples were obtained from three size fractions over period nearly months at an camp deployed on...
Abstract. The Green Edge project was designed to investigate the onset, life, and fate of a phytoplankton spring bloom (PSB) in Arctic Ocean. lengthening ice-free period warming seawater, amongst other factors, have induced major changes Ocean biology over last decades. Because PSB is at base food chain, it crucial understand how environment will affect it. large multidisciplinary, collaborative bringing researchers technicians from 28 different institutions seven countries together, aiming...
The mangrove's sediments from the coastal areas under human activities may contain significant contaminations by hydrocarbons, even when there are no visual evidences of it. microorganisms essential to these ecosystems, especially in control their chemical environment. Sediment samples were collected two regions different environment conditions (pristine and contaminated) Paranaguá Estuarine Complex (Paranaguá Bay Laranjeiras Bay), Brazil. Aliphatic hydrocarbons determined GC-FID assess...
Abstract Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under pack ice. An extensive culturing effort was conducted before and during a bloom in Baffin Bay between April July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single pipetting serial dilution. Although all three techniques yielded most common organisms, each technique retrieved specific taxa, highlighting importance of using several methods to...
The haptophyte genus Pseudohaptolina (formerly Chrysochromulina clade B1-3) currently harbours two species: arctica and sorokinii. In addition, birgeri is expected to belong this due its morphological similarity P. sorokinii, but it has not yet been genetically characterised. A strain belonging was brought into culture from Arctic waters, characterised by 18S 28S rRNA gene sequencing as well optical transmission electron microscopy, deposited in the Roscoff Culture Collection with code...
For metabarcoding purpose, the first step involves amplification by PCR of a given gene region (for example V4 or V9 18S rRNA gene) itself if its size does not exceed 600bp (the longest fragment that can be sequenced Illumina technology). The defined forward and reverse primers are complementary upstream downstream interest, needs to designed with overhang adapters which will used in subsequent limited‐cycle step, order add dual‐index barcodes flow cell adapters. To design illumina primers,...
In this work, the density of bacterioplankton, bacterial biomass and environmental variables were monitored in two seasons (summer winter), times each month (spring tide neap sampling), over a 12 h period, comprising tidal cycle (semidiurnal), from subsurface bottom waters, fixed station Estuarine Turbidity Maximum Zone (ETMZ) Paranaguá Bay, Brazil. The data treated with multivariate analyses methods order to indentify key controlling factors community dynamics. microbial seemed be...
Marine sponges are dominant components of Antarctic benthos and representative the high endemism that characterizes this environment. All microbial groups part sponge holobionts, but eukaryotes have been studied less, their symbiotic role still needs to be better understood. Here, we characterize dynamics associated with sponges, focusing on dinoflagellates over three summer periods understand members, interannual variations, trophic lifestyle strategies.
Abstract. The Green Edge initiative was developed to investigate the processes controlling primary productivity and fate of organic matter produced during Arctic phytoplankton spring bloom (PSB) determine its role in ecosystem. Two field campaigns were conducted 2015 2016 at an ice camp located on landfast sea southeast Qikiqtarjuaq Island Baffin Bay (67.4797N, 63.7895W). During both expeditions, a large suite physical, chemical biological variables measured beneath consolidated cover from...
Abstract. The Green Edge project was designed to investigate the onset, life and fate of a phytoplankton spring bloom (PSB) in Arctic Ocean. lengthening ice-free period warming seawater, amongst other factors, have induced major changes arctic ocean biology over last decades. Because PSB is at base Ocean food chain, it crucial understand how environment will affect it. large multidisciplinary collaborative bringing researchers technicians from 28 different institutions seven countries,...
Abstract Phytoplankton under-ice blooms have been recently recognized as an important Arctic phenomenon for global primary production and biogeochemical cycling. Drastic sea-ice decline in both extension thickness enables the development of early blooms, sometimes hundreds kilometers beneath pack ice. Baffin Bay is a semi-enclosed sea where North Atlantic water masses interact. It totally covered by March ice-free August/September. In present work, we investigated phytoplankton community...