A5078351196- Algal biology and biofuel production
- Geology and Paleoclimatology Research
- Geomagnetism and Paleomagnetism Studies
- Photosynthetic Processes and Mechanisms
- Marine and coastal plant biology
- Geological and Geochemical Analysis
- earthquake and tectonic studies
- Geological and Geophysical Studies
- Plant Stress Responses and Tolerance
- Microbial Community Ecology and Physiology
- Plant Molecular Biology Research
- Methane Hydrates and Related Phenomena
- Magnetic and Electromagnetic Effects
- Marine and environmental studies
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Plant Reproductive Biology
- Geological Studies and Exploration
- Aquatic Ecosystems and Phytoplankton Dynamics
- Coastal wetland ecosystem dynamics
- Pleistocene-Era Hominins and Archaeology
- Marine Biology and Environmental Chemistry
- Genomics and Phylogenetic Studies
- Seaweed-derived Bioactive Compounds
- Geological and Geophysical Studies Worldwide
The University of Tokyo
2013-2024
Japan Aerospace Exploration Agency
2022-2023
Japan Science and Technology Agency
2013-2019
Japan Space Forum
2019
Tohoku University
1972-2016
Sphere Institute
2015
Geological Survey of Japan
2014
Iwate University
2006-2010
Midorigaoka Hospital
2008
RELX Group (Netherlands)
2008
Abstract Plant freezing tolerance involves the prevention of lethal freeze-induced damage to plasma membrane. We hypothesized that plant membrane resealing, which, in animal cells, is accomplished by calcium-dependent exocytosis following mechanical disruption In Arabidopsis thaliana protoplasts, extracellular calcium enhanced not only but also electroporation, which typically punctures However, did enhance survival when protoplasts were exposed osmotic stress mimicked dehydration....
Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, starch productivity levels. However, further biotechnological exploitation has been hampered by lack genomic information.Here, we sequenced whole genome transcriptome, analyzed behavior P. kessleri NIES-2152 under lipid production-inducing...
Phosphorus is an essential element for life on earth and also important modern agriculture, which dependent inorganic fertilizers from phosphate rock. Polyphosphate a biological polymer of residues, accumulated in organisms during the wastewater treatment process to enhance phosphorus removal. Here, we investigated relationship between polyphosphate accumulation electron-dense bodies green alga Parachlorella kessleri. Under sulfur-depleted conditions, some symporter genes were upregulated,...
MIZ1 is encoded by a gene essential for root hydrotropism in Arabidopsis. To characterize the property of MIZ1, we used transgenic plants expressing GFP-tagged (MIZ1-GFP) and mutant (MIZ1(G235E)-GFP) miz1-1 mutant. Although both chimeric genes were transcribed, translational products MIZ1(G235E)-GFP did not accumulate roots. Moreover, MIZ1-GFP complemented phenotype but MIZ1(G235E)-GFP. The signal corresponding to was detected at high levels cortical cells lateral cap accumulated...
The correct localization of integral membrane proteins to subcellular compartments is important for their functions. Synaptotagmin contains a single transmembrane domain that functions as type I signal-anchor sequence in its N terminus and two calcium-binding domains (C2A C2B) C terminus. Here, we demonstrate the an Arabidopsis synaptotagmin homolog, SYT1, plasma (PM) modulated by tandem C2 domains. An analysis roots transformant-expressing green fluorescent protein-tagged SYT1 driven native...
Summary Septins are a group of GTP ‐binding proteins that multi‐functional, with well‐known role in cytokinesis animals and fungi. Although the functions septins have been thoroughly studied opisthokonts (fungi animals), function evolution plant/algal not as well characterized. Here we describe septin localization expression green algae N annochloris bacillaris M arvania geminata . The present data suggest localize at division site when occurs. In addition, show homologs may be found only...
The evolution of sex chromosomes and mating loci in organisms with UV systems sex/mating type determination haploid phases via genes on is not well understood. We report the structure (MT) locus its evolutionary history green seaweed Ulva partita, which a multicellular organism an isomorphic haploid-diploid life cycle phase. Comprehensive comparison total 12.0 16.6 Gb genomic next-generation sequencing data for mt- mt+ strains identified highly rearranged MT 1.0 1.5 Mb size containing 46 67...
We report a paleomagnetic secular variation in the Society Islands, French Polynesia for past 5 Ma. Paleomagnetic measurements were performed on volcanic rocks applying thermal and alternating field demagnetizations principal-component analysis. As result, 82 normal, 48 reversed, 10 intermediate directions observed after correction of site localities absolute motion Pacific plate. A mean VGP combined data set normal reversed polarities is located at 87.5°N 4.0°W (A95 = 2.3°), which close to...
We used a microplate-based method to quantify microalgal lipids with Nile Red staining and fluorescence microplate reader. However, starch that combines microplates has not been reported. Therefore, we examined quantification of using Lugol staining. Neither nor accumulated during the zero phase cultured Parachlorella kessleri, only phase, was subsequently lost oil phase. The quantities were measured method, which indicated linear production within limited ranges (lipids, 0.071–0.380 g mL−1;...
SUMMARY Although the green seaweed Ulva is one of most common seaweeds in coastal regions with well‐studied ecological characteristics, few reverse genetic technologies have been developed for it. The clustered regularly interspaced short palindromic repeats (CRISPR)‐Cas9 system a simple genome‐editing technology based on ribonucleoprotein (RNP) complex composed an endonuclease and programmable RNA to target particular DNA sequences. Genome editing makes it possible generate mutations gene...
Extracellular freezing in plants results dehydration and mechanical stresses upon the plasma membrane. Plants that acquire enhanced tolerance after cold acclimation can withstand these two physical stresses. To understand to freeze-induced stresses, cryobehavior of membrane was observed using protoplasts isolated from cold-acclimated Arabidopsis thaliana leaves with combination a lipophilic fluorescent dye FM 1-43 cryomicroscopy. We found many vesicular structures appeared cytoplasmic region...
Apomixis is an asexual reproduction system without fertilization, which important proliferation strategy for plants and algae. Here, we report on the apomeiosis in green seaweed Ulva prolifera, has sexual obligate populations. Genomic PCR of mating type (MT)-locus genes revealed thalli carrying both MT genomes. Observation chromosomes during formation each reproductive cell that cells performed chromosome reduction. Moreover, genotyping laboratory-cultured sporophytic produced not only...
In this report, Ulva partita Ichihara, sp. nov. (Ulvales, Ulvophyceae) was described from Japanese seashores. This species characterized by (i) frequent branches in the basal region, but not middle to upper (ii) being up 20 cm height and 5 mm diameter, (iii) containing chloroplasts covering outer cell wall regions of thalli, with many leaning one side cells region. is distinguished morphologically similar based on branching pattern, early development gamete size. Molecular phylogenetic...
Two FtsZ paralogues ( NbFtsZ1 and NbFtsZ2 ) were isolated from the unicellular green alga Nannochloris bacillaris Naumann. These sequences encoded proteins of 435 439 amino acids with tubulin signature motifs (GGGTG[T/S]G), which are important for GTP binding activity. had four three introns, respectively, two different putative core promoters; a TATA box (TATAAAA) an initiator element (CCCAGG) located 40 bp 80 upstream coding regions , respectively. Southern blot hybridization...