A5087268447- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Light effects on plants
- Cancer Immunotherapy and Biomarkers
- Photosynthetic Processes and Mechanisms
- Lung Cancer Treatments and Mutations
- Atrial Fibrillation Management and Outcomes
- Biofuel production and bioconversion
- Metastasis and carcinoma case studies
- Lung Cancer Research Studies
- Tracheal and airway disorders
- Plant Water Relations and Carbon Dynamics
- Plant Reproductive Biology
- Bioenergy crop production and management
- Lignin and Wood Chemistry
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Cardiac Arrhythmias and Treatments
- Plant tissue culture and regeneration
- Streptococcal Infections and Treatments
- Pharmacovigilance and Adverse Drug Reactions
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Otolaryngology and Infectious Diseases
- Cancer Genomics and Diagnostics
Toyama University Hospital
2020-2025
Toyama Prefectural Central Hospital
2022-2024
Kobe Central Hospital
2024
Kagawa Prefectural Central Hospital
2019-2024
Kobe University Hospital
2024
Hyogo Prefectural Awaji Medical Center
2023-2024
Kobe Kaisei Hospital
2024
St. Mary Medical Center
2024
Kashiwa Municipal Hospital
2024
Tokyo Metropolitan Tama-Hokubu Medical Center
2024
Wood fibers form thick secondary cell wall (SCW) in xylem tissues to give mechanical support trees. NAC SECONDARY WALL THICKENING PROMOTING FACTOR3/SECONDARY WALL-ASSOCIATED DOMAIN PROTEIN 1 (NST3/SND1) and NST1 were identified as master regulators of SCW formation fiber cells the model plant Arabidopsis thaliana. In Populus species, four NST/SND orthologs have been conserved coordinately control wood phloem fibers. However, it remains be elucidated whether other cells, such ray parenchyma...
• LHY/CCA1 genes play a key role in the plant circadian clock system and are highly conserved among species. However, evolutionary process of gene family remains unclear angiosperms. To obtain details phylogeny these genes, this study characterized model woody plant, Populus tree. The angiosperm was elucidated using three approaches: comparison exon–intron structures, reconstruction phylogenetic trees examination syntenic relationships. In addition, molecular rates expression patterns LHYs...
Abstract Background Plant circadian clocks regulate many photoperiodic and diurnal responses that are conserved among plant species. The clock system has been uncovered in the model plant, Arabidopsis thaliana , using genetics systems biology approaches. However, it is still not clear how had organized evolutionary history of plants. We recently revealed molecular phylogeny LHY/CCA1 genes, one essential components system. aims this study to reconstruct phylogenetic relationships angiosperm...
Abstract Lignocellulose, composed of cellulose, hemicellulose and lignin, in the secondary cell wall constitutes wood is most abundant form biomass on Earth. Enhancement accumulation may be an effective strategy to increase as well strength, but currently only limited research has been undertaken. Here, we demonstrated that OsSWN1, orthologue rice N AC S econdary-wall T hickening factor (NST) transcription factor, effectively enhanced formation Arabidopsis inflorescence stem poplar ( Populus...
Severe odontogenic infections in the head and neck region, especially necrotizing soft tissue infection (NSTI) deep abscess, are potentially fatal due to their delayed diagnosis treatment. Clinically, it is often difficult distinguish NSTI abscess its early stage from cellulitis, decision perform contrast-enhanced computed tomography imaging for detection a challenge. This retrospective case-control study aimed examine utility of routine blood tests as an adjunctive diagnostic tool region...
Background/Aim: There is insufficient evidence regarding the optimal treatment for squamous cell lung cancer patients with epidermal growth factor receptor (EGFR) mutations, and a standard strategy has not been established. Case Report: A 60-year-old woman was diagnosed advanced EGFR-mutant cancer. She treated EGFR tyrosine kinase inhibitors then received combined therapy of cytotoxic agents plus immune checkpoint as third-line therapy. fourth-line combination cisplatin, gemcitabine,...
In plant cell walls, lignin, cellulose, and the hemicelluloses form intricate three-dimensional structures. Owing to its complexity, lignin often acts as a bottleneck for efficient utilization of polysaccharide components biochemicals functional materials. A promising approach mitigate and/or overcome recalcitrance is qualitative quantitative modification by genetic engineering. Feruloyl-CoA 6′-hydroxylase (F6′H1) 2-oxoglutarate-dependent dioxygenase that catalyzes conversion feruloyl-CoA,...
Abstract Understanding the timely regulation of plant growth and phenology is crucial for assessing a terrestrial ecosystem’s productivity carbon budget. The circadian clock, system genetic oscillators, acts as ‘Master Ceremony’ during physiological processes. mechanism particularly elusive in trees despite its relevance. primary secondary tree growth, leaf senescence, bud set, burst timing were investigated 68 constructs transformed into Populus hybrids compared with untransformed or...
The genus Populus is accepted as a model system for molecular tree biology. To investigate gene functions in spp. trees, generating stable transgenic lines the common technique functional genetic studies. However, limited number of genes have been targeted due to lengthy process. Transient transformation assays complementing significant advantages rapid vivo assessment function. aim this study develop simple and efficient transient hybrid aspen provide its potential applications genomic...
EPF1-EPF2 and EPFL9/Stomagen act antagonistically in regulating leaf stomatal density. The aim of this study was to elucidate the evolutionary functional divergence EPF/EPFL family genes. Phylogenetic analyses showed that AtEPFL9/Stomagen-like genes are conserved only vascular plants closely related AtEPF1/EPF2-like Modeling peptides share a common 3D structure is constituted scaffold loop. Molecular dynamics simulation suggested form an additional disulfide bond their loop regions show...
Lignin is a phenolic polymer deposited in the plant cell wall, and mainly polymerized from three canonical monomers (monolignols), i.e. p-coumaryl, coniferyl sinapyl alcohols. After polymerization, these alcohols form different lignin substructures. In dicotyledons, monolignols are biosynthesized phenylalanine, an aromatic amino acid. Shikimate acts at two positions route to building blocks. It part of shikimate pathway that provides precursor for biosynthesis involved transesterification...
Abstract Objective Studies have suggested the potential efficacy of immune checkpoint inhibitors (ICIs) for pulmonary sarcomatoid carcinoma. This multicenter observational study was conducted to evaluate systemic ICI therapy and chemoradiation followed by durvalumab Methods We analyzed data patients with carcinoma who received or between 2016 2022. Results In this study, a total 22 four were analyzed. therapy, median progression‐free survival after initiation 9.6 months, overall did not...
The correct localization of integral membrane proteins to subcellular compartments is important for their functions. Synaptotagmin contains a single transmembrane domain that functions as type I signal-anchor sequence in its N terminus and two calcium-binding domains (C2A C2B) C terminus. Here, we demonstrate the an Arabidopsis synaptotagmin homolog, SYT1, plasma (PM) modulated by tandem C2 domains. An analysis roots transformant-expressing green fluorescent protein-tagged SYT1 driven native...
Abstract Trees are carbon dioxide sinks and major producers of terrestrial biomass with distinct seasonal growth patterns. Circadian clocks enable the coordination physiological biochemical temporal activities, optimally regulating multiple traits including growth. To dissect clock's role in growth, we analysed Populus tremula × P. tremuloides trees impaired clock function due to down‐regulation central components. late elongated hypocotyl ( lhy‐10 ) trees, which expression LHY1 LHY2 is...
Stomata in the plant epidermis open response to light and regulate CO2 uptake for photosynthesis transpiration of water nutrients from roots. Light-induced stomatal opening is mediated by activation plasma membrane (PM) H+-ATPase guard cells. Overexpression PM cells promotes light-induced opening, enhancing growth Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing (AHA2) under strong cell promoter GC1 (AtGC1) showed enhanced photosynthesis, growth. First, we...
Plants balance water availability with gas exchange and photosynthesis by controlling stomatal aperture. This control is regulated in part the circadian clock, but it remains unclear how signalling pathways of daily rhythms are integrated into stress responses. The serine/threonine protein kinase OPEN STOMATA 1 (OST1) contributes to regulation closure via activation S-type anion channels. OST1 also mediates gene response ABA/drought stress. We show that ZEITLUPE (ZTL), a blue light...
Abstract The circadian clock of the model plant Arabidopsis (Arabidopsis thaliana) is made up a complex series interacting feedback loops whereby proteins regulate their own expression across day and night. early bird (ebi) mutation that causes to speed up: ebi plants have short periods, phase gene expression, are flowering. We show EBI associates with ZEITLUPE (ZTL), known act in as posttranslational mediator protein degradation. However, not degraded by its interaction ZTL. Instead, ZTL...
We previously succeeded in enhancing wood formation of transgenic poplar plants by overexpressing secondary wall NAM/ATAF/CUC (NAC) domain protein 1 from Oryza sativa (OsSWN1), a transcription factor 'master regulator' cell rice, under control the fiber preferential NST3/SND1 promoter Arabidopsis. Transgenic had an increased thickness and density individual cells xylem stems as well density. OsSWN1 triggers induction polysaccharide lignin biosynthetic gene expressions, however, resulting no...
Background Necrotizing fasciitis (NF) is an acute and life-threatening soft-tissue infection however rarely seen in oro-cervical region. Therefore, the details of NF (OCNF) are not well known. The purpose this study was to investigate characteristics OCNF by comparing it with severe cellulitis region (OCSC) or other body regions (e.g., limb, perineum, trunk) (BNF), respectively. Materials methods At first, various risk factors for (OCSI; composed OCSC), including neutrophil-to-lymphocyte...
Background: Among patients with non-small cell lung cancer (NSCLC), we compared the progression-free survival (PFS) and proportion of acquisition T790M mutation epidermal growth receptor gene (EGFR) after first-line treatment factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) in patient groups without tumor expression programmed death ligand-1 (PD-L1). Methods: Data EGFR-mutant NSCLC were retrospectively analyzed. Tumor PD-L1 was evaluated by immunohistochemistry using 22C3 antibody....