- Insect Pheromone Research and Control
- Insect and Pesticide Research
- Neurobiology and Insect Physiology Research
- Plant and animal studies
- Insect-Plant Interactions and Control
- Enzyme Production and Characterization
- Insect and Arachnid Ecology and Behavior
- Insect Pest Control Strategies
- Pancreatic function and diabetes
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Pancreatitis Pathology and Treatment
- Legume Nitrogen Fixing Symbiosis
- Molecular spectroscopy and chirality
- Photoreceptor and optogenetics research
- Plant Gene Expression Analysis
- Pineapple and bromelain studies
- Insect Resistance and Genetics
- Molecular Biology Techniques and Applications
- Essential Oils and Antimicrobial Activity
- Traditional and Medicinal Uses of Annonaceae
- bioluminescence and chemiluminescence research
- Calcium signaling and nucleotide metabolism
- Synthetic Organic Chemistry Methods
- Neuropeptides and Animal Physiology
Tokyo University of Agriculture and Technology
2011-2024
Purdue University West Lafayette
2024
Magneto-Inertial Fusion Technologies (United States)
2024
The University of Tokyo
2014-2019
Food Research Institute
2009-2013
Food Research Institute
2009-2013
Osaka University of Pharmaceutical Sciences
2008
University of Helsinki
2008
Nagoya University
2008
SPring-8
2005
The C10-C18 unsaturated, acyclic, aliphatic compounds that contain an oxygenated functional group (alcohol, aldehyde, or acetate ester) are a major class of sex pheromones produced by female moths. In the biosynthesis these pheromone components, key enzyme required to produce groups is fatty-acyl reductase (FAR). This converts precursors their corresponding alcohols, which, depending on moth species, can then be acetylated oxidized aldehydes. Despite significant role this has in generating...
The straight-chain C 10 to 18 unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated 16 fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis these components, various combinations limited chain-shortening and regio- stereospecific desaturation reactions significantly contribute production vast number species-specific in Lepidoptera. Biosynthesis silkmoth...
Flagellar motility and its glycosylation are indispensable for the virulence of Pseudomonas syringae pv. tabaci 6605. Six serine residues flagellin protein at positions 143, 164, 176, 183, 193 201 glycosylated, glycan structure was determined to consist a trisaccharide two L-rhamnosyl modified 4-amino-4,6-dideoxyglucosyl (viosamine) residue. To investigate structures attached other identify glycans important virulence, Ser/Ala-substituted mutants were generated. mutant strains that each...
Lignin is a phenolic polymer deposited in the plant cell wall, and mainly polymerized from three canonical monomers (monolignols), i.e. p-coumaryl, coniferyl sinapyl alcohols. After polymerization, these alcohols form different lignin substructures. In dicotyledons, monolignols are biosynthesized phenylalanine, an aromatic amino acid. Shikimate acts at two positions route to building blocks. It part of shikimate pathway that provides precursor for biosynthesis involved transesterification...
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Circadian rhythms are indispensable intrinsic programs that regulate the daily rhythmicity of physiological processes, such as feeding and sleep. The cricket has been employed a model organism for understanding neural mechanisms underlying circadian in insects. However, previous studies measuring rhythm-controlled behaviours only analysed locomotive activity using seesaw-type infrared sensor-based actometers. Meanwhile, advances deep learning techniques have made it possible to analyse...
During the chemical and biochemical decomposition of lignocellulosic biomasses, lignin is highly recalcitrant. Genetic transformation plants to qualitatively and/or quantitatively modify may reduce these recalcitrant properties. Efficient discovery genes achieve manipulation thus required. To screen for new recalcitrance, we heterologously expressed 50 enzymatic under control a cinnamate 4-hydroxylase (C4H) gene promoter, derived from hybrid aspen, which preferentially active in tissues with...
SUMMARY Type IV pilin (PilA) is a major constituent of pilus and required for bacterial biofilm formation, surface motility virulence. It known that mature PilA produced by cleavage the short leader sequence precursor, followed methylation N‐terminal phenylalanine. The molecular mass protein from tobacco pathogen Pseudomonas syringae pv. tabaci 6605 ( Pta 6605) has been predicted to be 12 329 Da its deduced amino acid sequence. Previously, we have detected as an approximately 13‐kDa...
Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and glycosylation was required for virulence. We further identified some glycosylation-related genes, including vioA, vioB, vioT, fgt1, fgt2. In this study, newly vioR vioM so-called island as biosynthetic genes of mVio Pta by the mass spectrometry (MS) glycan respective mutants. Furthermore, characterization...
Since the first identification of bombykol, sex pheromones about 700 moth species have been elucidated. Additionally, field evaluations synthetic and their related compounds revealed male attraction another 1,300 species. These attractants are listed on web-sites, "Pheromone Database, Part I." Pheromone components classified according to chemical structures into two major groups (Types I II) miscellaneous. Based our previous review published in 2004, studies reported during last decades...
Glycogen phosphorylase b was purified 70- to 90-fold from skeletal muscle of rainbow trout (Salmo gairdneri). The enzyme exhibited maximal activity near pH 6.8 at 37°. Of several 5′-nucleotides tested, only 5′-AMP caused stimulation b. K m value for glucose-1-phosphate 10–15 mM, and 5′-AMP, 0.2–0.4 mM. Glucose (25 mM) ATP (5 were both inhibitory, but glucose-6-phosphate had no effect. Inactive converted the active form in vivo by subjecting a fish physical exercise. conversion also catalyzed...
Abstract Increases in coniferaldehyde content, a minor lignin residue, significantly improves the sustainable use of plant biomass for feed, pulping, and biorefinery without affecting growth yields. Herein, different analytical methods are compared validated to distinguish from other residues. It is shown that specific genetic pathways regulate amount, linkage, position within polymer each cell type. This cellular regulation offers new possibilities designing novel targeted industrial uses.
Several geometrical isomers of 3,13- and 2,13-octadecadien-1-ols their acetates were synthesized starting from 1,8-octanediol or 1,9-nonanediol utilizing acetylene coupling reactions. In addition to commercially available compounds, all each dienyl compound analyzed by NMR GC-MS accumulate chemical data for studies sex pheromones secreted clearwing moths classified into the family Sesiidae Lepidoptera. Although acetoxy derivatives 2,13-dienes showed almost same mass spectra, alcohols...