A5086125482- Plant Molecular Biology Research
- Plant Reproductive Biology
- Tree Root and Stability Studies
- Plant Gene Expression Analysis
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Material Properties and Processing
- Biofuel production and bioconversion
- Polysaccharides Composition and Applications
- Lignin and Wood Chemistry
- Plant Water Relations and Carbon Dynamics
- Aeolian processes and effects
- Leaf Properties and Growth Measurement
- Bamboo properties and applications
- Photosynthetic Processes and Mechanisms
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
Nara Institute of Science and Technology
2020-2024
Kumamoto University
2023-2024
National Institute of Advanced Industrial Science and Technology
2018-2021
Rikkyo University
2018-2020
National Institute for Basic Biology
2012-2018
Kyoto University
2012
During leaf development in flowering plants, adaxial (upper) and abaxial (lower) side–specific genes are responsible for blade outgrowth, which takes places predominantly the lateral direction, margin as well differentiation of tissues. However, underlying mechanisms poorly understood. Here, we show that two WUSCHEL-RELATED HOMEOBOX (WOX) genes, PRESSED FLOWER (PRS)/WOX3 WOX1, encoding homeobox transcription factors, act outgrowth downstream adaxial/abaxial polarity establishment. The...
Wood fibers form thick secondary cell wall (SCW) in xylem tissues to give mechanical support trees. NAC SECONDARY WALL THICKENING PROMOTING FACTOR3/SECONDARY WALL-ASSOCIATED DOMAIN PROTEIN 1 (NST3/SND1) and NST1 were identified as master regulators of SCW formation fiber cells the model plant Arabidopsis thaliana. In Populus species, four NST/SND orthologs have been conserved coordinately control wood phloem fibers. However, it remains be elucidated whether other cells, such ray parenchyma...
During the chemical and biochemical decomposition of lignocellulosic biomasses, lignin is highly recalcitrant. Genetic transformation plants to qualitatively and/or quantitatively modify may reduce these recalcitrant properties. Efficient discovery genes achieve manipulation thus required. To screen for new recalcitrance, we heterologously expressed 50 enzymatic under control a cinnamate 4-hydroxylase (C4H) gene promoter, derived from hybrid aspen, which preferentially active in tissues with...
Blade outgrowth and region-specific cell differentiation are crucial events during the early development of plant leaves, progression both these requires a normal adaxial-abaxial pattern. In recent study, we had demonstrated that two WUSCHEL-RELATED HOMEOBOX (WOX) family genes, i.e., PRESSED FLOWER (PRS) WOX1, act redundantly in blade patterning. During leaf development, genes expressed domain between adaxial abaxial domains, designated "the middle domain." Together with additional data,...
Organ size regulation is dependent on the precise spatial and temporal of cell proliferation expansion. A number transcription factors have been identified that play a key role in determination aerial lateral organ size, but their functional relationship to various chromatin modifiers has not well understood. To understand how leaf regulated, we previously isolated oligocellula1 (oli1) mutant Arabidopsis thaliana develops smaller first leaves than wild type (WT) mainly due reduction number....
Abstract Leaves have evolved shape diversity, ranging from simple leaves with a smooth margin to complicated shapes toothed/serrated, lobed, and dissected leaflets. In the model plant Arabidopsis thaliana producing serrated margin, boundary regulatory factor genes CUP SHAPED COTYLEDON 2 (CUC2) CUC3 play important roles in promoting leaf initiation maintenance of serration. Stem cell-related WUSCHEL-RELATED HOMEOBOX1 (WOX1) PRESSED FLOWER (PRS)/WOX3 are also essential for morphogenesis, but...
The WUSCHEL-RELATED HOMEOBOX1 (WOX1) transcription factor and its homolog PRESSED FLOWER (PRS) are multifunctional regulators of leaf development that act as transcriptional repressors. These genes promote cell proliferation under certain conditions, but the related molecular mechanisms not well understood. Here, we present a new function for WOX1 in proliferation. To identify downstream genes, performed microarray analysis shoot apices transgenic Arabidopsis thaliana lines harboring...
Mechanical properties are rarely used as quantitative indices for the large-scale mutant screening of plants, even in model plant Arabidopsis thaliana. The mechanical stems generally influence their vibrational characteristics. Here, we developed Python-based software, named AraVib, high-throughput analysis free vibrations stems, focusing specifically on stem vibrations, and its extended version, AraVibS, to identify mutants with altered properties. These programs can be without knowledge...
Abstract The cortical motor cells (CMCs) in a legume pulvinus execute the reversible deformation leaf movement that is driven by changes turgor pressure. In contrast to underlying osmotic regulation property, cell wall structure of CMCs contributes has yet be characterized detail. Here, we report circumferential slits with low levels cellulose deposition, which are widely conserved among species. This unique and distinct from any other primary walls reported so far; thus, named them...
The plastid evolved from a symbiotic cyanobacterial ancestor and is an essential organelle for plant life, but its developmental roles in roots have been largely overlooked. Here, we show that translation connected to the stem-cell patterning lateral root primordia. RFC3 gene encodes plastid-localized protein conserved bacterial ribosomal S6 of β/γ proteobacterial origin. rfc3 mutant developed with disrupted associated decreased leaf photosynthetic activity, reduced accumulation rRNAs roots,...
Arabidopsis thaliana transcription factors belonging to the ERFIIId and ERFIIIe subclade (ERFIIId/e) of APETALA 2/ethylene response factor (AP2/ERF) family enhance primary cell wall (PCW) formation. These activate expression genes encoding PCW-type cellulose synthase (CESA) subunits other for PCW biosynthesis. In this study, we show that fiber-specific ERF035-VP16 ERF041-VP16, which are VP16-fused proteins ERFIIId/e members, promote thickening in a wild-type background with concomitant...
The mechanical strength of a plant stem (a load-bearing organ) helps the resist drooping, buckling and fracturing. We previously proposed method for quickly evaluating stiffness an inflorescence in model Arabidopsis thaliana based on measuring its natural frequency free-vibration test. However, relationship between flexural rigidity stems was unclear. Here, we compared our described test with three-point bending test, most popular calculating A. stems, examined extent to which results were...
Plastid ribosome biogenesis is important for plant growth and development. REGULATOR OF FATTY ACID COMPOSITION3 (RFC3) a member of the bacterial ribosomal protein S6 family lateral root rfc3-2 dramatically reduces plastid rRNA level produces roots that lack stem cells. In this study, we isolated suppressor rfc three2 (sprt2) mutant enabled recovery most rfc3 phenotypes, including abnormal primary development reduced level. Northern blotting showed immature mature levels were reduced, with...
Abstract The cortical motor cells (CMCs) in a legume pulvinus execute the reversible deformation leaf movement that is driven by changes turgor pressure. In contrast to underlying osmotic regulation proper, cell wall structure of CMCs contributes has yet be characterized detail. Here, we report circumferential slits with low levels cellulose deposition. This unique and distinct from any other primary walls reported so far; thus, named them “pulvinar slits.” Notably, predominantly detected...
Abstract Mechanical properties are rarely used as quantitative indices for the large-scale mutant screening of plants, even in model plant Arabidopsis thaliana . The mechanical stems generally influence their vibrational characteristics. Here, we developed Python-based software, named AraVib, high-throughput analysis free vibrations stems, focusing specifically on stem vibrations, and its extended version, AraVibS, to identify mutants with altered properties. These programs can be without...