A5015604527- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Advanced Electron Microscopy Techniques and Applications
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Antibiotics Pharmacokinetics and Efficacy
- Biocrusts and Microbial Ecology
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Synthesis of Organic Compounds
- Phytase and its Applications
- Magnetic and Electromagnetic Effects
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Plant Reproductive Biology
- Synthesis of β-Lactam Compounds
- Phenothiazines and Benzothiazines Synthesis and Activities
- Plant responses to water stress
- Polysaccharides Composition and Applications
- Electron and X-Ray Spectroscopy Techniques
- Lipid metabolism and biosynthesis
Saitama University
2015-2025
Synthetic Biologics (United States)
2018
Kookmin University
2018
Graduate School USA
2013
Tokyo University of Agriculture and Technology
1998
Chiba University
1993-1996
Urawa University
1989-1995
University of Wisconsin–Madison
1985-1994
Toho University
1976-1994
Ajinomoto (Japan)
1991
Summary Lipid transfer proteins (LTPs), mediate the of phospholipids between membranes in vitro. However, vivo function LTPs is not known. To determine precise location a non‐specific LTP from Arabidopsis , cDNA clone was used to produce an LTP:protein A fusion. Antibodies raised against fusion were localize by immunoelectron microscopy. found be located cell wall, mainly epidermal cells. This appears inconsistent with proposed role protein intracellular lipid transfer.
The brittle culm (bc) mutants of Gramineae plants having skeletal structures are valuable materials for studying secondary cell walls. In contrast to other recessive bc mutants, rice Bc6 is a semi-dominant mutant with easily breakable plant bodies. this study, the gene was cloned by positional cloning. encodes cellulose synthase catalytic subunit, OsCesA9, and has missense mutation in its highly conserved region. culms mutant, proportion reduced 38%, while that hemicellulose increased 34%....
Higher plants have two metabolic pathways for isoprenoid biosynthesis: the cytosolic mevalonate (MVA) pathway and plastidal non-mevalonate (MEP) pathway. Despite compartmentalization of these pathways, flow occurs between them. However, little is known about mechanisms that regulate cross-talk. To identify such regulatory mechanisms, we isolated characterized Arabidopsis T-DNA insertion mutant lovastatin insensitive 1 (loi1), which resistant to clomazone, inhibitors MVA MEP respectively. The...
All known cyanobacterial genomes possess multiple copies of genes encoding AbrB-like transcriptional regulators, as cyAbrBs, which are distinct from those conserved among other bacterial species. In this study, we addressed the physiological roles Sll0822 and Sll0359, two cyAbrBs in Synechocystis sp. strain PCC 6803, under nonstress conditions (20 μmol photons m⁻² s⁻¹ ambient CO₂). When sll0822 gene was disrupted, expression levels nitrogen-related such urtA, amt1, glnB significantly...
Carboxysomes in rapidly frozen ice-embedded whole cells of the cyanobacterium Synechococcus sp. strain PCC 7942 were visualized by recently developed Hilbert differential contrast transmission electron microscope. Structural details carboxysomes especially clearly ruptured cells. The novel microscopy exhibited paracrystalline arrays molecules enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase much better than conventional with ultrathin sections carboxysome was surrounded a 5- to...
We studied the heat resistance and range of growth temperature o gram-negative rods to find one bacterial factors governing their infectivity in exogenous endogenous infections predisposed patients. Escherichia coli, Klebsiella pneumoniae, serratia marcescens, Pseudomonas aeruginosa, Acinetobacter calcoaceticus grew equally well at 25, 30, 37, 42 C. Among other sugar non-fermenting rods, six species showed suppressed either or both ends incubation range. All tested were killed within 30 min...
Several brittle culm (bc) mutants known in grasses are considered excellent materials to study the process of secondary cell wall formation. The phenotype rice bc5 (brittle node) mutant appears exclusively developed nodes, which is distinct from other bc (bc1, 2, 3, 4, 6 and 7) that show culms leaves. To address defects node-specific formation, we analyzed tissue morphology composition. mutation was found affect deposition node sclerenchyma tissues at 1 week after heading, stage sugar...
Abstract cyAbrB is a transcriptional regulator unique to and highly conserved among cyanobacterial species. A gene-disrupted mutant of cyabrB2 (sll0822) in Synechocystis sp. PCC 6803 exhibited severe growth inhibition abnormal accumulation glycogen granules within cells under photomixotrophic conditions. Within 6 h after the shift conditions, sodium bicarbonate-dependent oxygen evolution activity markedly declined ƊcyabrB2 mutant, but decrease methyl viologen-dependent electron transport was...
We characterized the pcb2 (pale-green and chlorophyll b reduced 2) mutant. found through electron microscopic observation that chloroplasts of mesophyll cells lacked distinctive grana stacks. High-performance liquid chromatography (HPLC) analysis showed mutant accumulated divinyl chlorophylls, relative amount was remarkably less than a. The responsible gene mapped in an area 190 kb length at upper arm 5th chromosome, comparison DNA sequences revealed a single nucleotide substitution causing...
Abstract Some cyanobacteria exhibit compaction of DNA in synchrony with their circadian rhythms accompanying cell division. Since the structure is transient, it has not yet been described detail. Here, we successfully visualize ultrastructure compacted cyanobacterium Synechococcus elongatus PCC 7942 under rigorous synchronized cultivation by means high-voltage cryo-electron tomography. In 3D reconstructions rapidly frozen cells, appears as an undulating rod resembling a eukaryotic condensed...
Abstract During the past 2 decades, a substantial body of structural, biochemical, and molecular evidence has been amassed in support hypothesis that charophycean green algae are closest extant protist relatives land plants (embryophytes). Charophycean include filamentous unicellular Zygnematales, represented by familiar Spirogyra desmids; relatively large complex Charales, such as Chara; less conspicuous, but well‐studied Coleochaete; several other well‐known taxa. Ultrastructural studies...
Abstract Although most cyanobacterial cells contain prominent polyphosphate bodies in the central cytoplasmic area enclosed by peripheral thylakoid membranes, their roles are not fully understood. Storing phosphate for nucleotide production might be one of important survival cells. As a step towards identifying possible contribution to DNA synthesis, relationship between and throughout cell division cycle Synechococcus elongatus PCC 7942 cultured under light/dark cycles was investigated with...
Using 18-day-old Arabidopsis thaliana seedlings grown under increased (780 p.p.m., experimental plants) or ambient (390 control CO2 conditions, we evaluated 14CO2 photoassimilation in and translocation from representative source leaves. The total amounts the third leaves of plants comparison with that found for plants, but rates were comparable first two groups. In contrast, labeled assimilates doubled group, whereas was, at best, passively enhanced even though their transcript levels...
Heterogeneous distribution of PSI and PSII in thick grana shade chloroplasts is argued to hinder spillover chlorophyll excitations from PSI. To examine this dogma, we measured fluorescence induction at 77 K 690 nm (PSII) 760 (mostly PSI) the leaf discs Spinacia oleracea, Cucumis sativus, shade-tolerant Alocasia odora, grown high low light, quantified their capacities. (FI) consists intrinsic (FIα) caused by spilt over (FIβ). When FI FII parameters between State 1 2, induced weak far-red blue...
ABSTRACT The molecular mechanisms underlying the synthesis and secretion of large cell wall polysaccharides in plant cells are not fully understood. In this study, we report that two atypical endo-β-1,4-mannanases (MANs), which secreted do degrade glucomannan wall, play a novel role synthesis. Among six MANs Arabidopsis, AtMAN2 AtMAN5 contain transmembrane (TM) domain at their N-terminal region instead signal peptide. Subcellular localization using MAN protein fused with fluorescent...
High-pressure freezing–freeze substitution (HPF-FS) methods were applied to germinating pea leaves. Good ultrastructural preservation without visible freezing damage was obtained up 200 μm in thickness. Compared conventional chemical fixation (CF), cellular membranes smoother undulation, and organelles appeared more turgid. The matrices of cytoplasm denser homogeneous. These features imply that HPF-FS samples retain substances ultrastructure closer the living state. There differences...