A5059886372- Pharmacological Effects of Natural Compounds
- Oral microbiology and periodontitis research
- Microbial Natural Products and Biosynthesis
- Pathogenesis and Treatment of Hiccups
- Bacterial Genetics and Biotechnology
- Oral and gingival health research
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Drug-Induced Hepatotoxicity and Protection
- Plant Disease Resistance and Genetics
- Microbial Metabolic Engineering and Bioproduction
- COVID-19 Clinical Research Studies
- Olfactory and Sensory Function Studies
- HIV/AIDS oral health manifestations
- Enzyme Structure and Function
- Oral Health Pathology and Treatment
- RNA and protein synthesis mechanisms
- Plant-based Medicinal Research
- Long-Term Effects of COVID-19
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- bioluminescence and chemiluminescence research
- Complementary and Alternative Medicine Studies
- Salivary Gland Disorders and Functions
- Periodontal Regeneration and Treatments
Tokyo Medical and Dental University
2021-2024
The University of Tokyo
2021-2024
Tohoku University Hospital
2016-2024
National Institute of Technology, Tsuruoka College
2015-2024
National Institute of Technology, Kagoshima College
2024
Tohoku University
2023
Nishi Niigata Chuo National Hospital
2023
Keio University
2005-2021
Niigata University
2012-2019
Kobe Women's University
2017
We developed a sample preparation protocol for rapid and unbiased analysis of the membrane proteome using an alimentary canal-mimicking system in which proteases are activated presence bile salts. In this protocol, immobilized trypsin is used deoxycholate lauroylsarcosine to increase digestion efficiency as well solubility proteins. Using 22.5 microg Escherichia coli whole cell lysate, we quantitatively demonstrated that proteins were extracted digested at same level soluble without any...
Abstract Background With the advent of metabolomics as a powerful tool for both functional and biomarker discovery, identification specific differences between complex metabolite profiles is becoming major challenge in data analysis pipeline. The task remains difficult, given datasets' size, complexity, common shifts migration (elution/retention) times samples analyzed by hyphenated mass spectrometry methods. Results We present Mathematica (Wolfram Research, Inc.) package MathDAMP...
Abstract Aim This study aimed to investigate the effects of diabetes care on periodontal inflammation. Materials and Methods prospective cohort included 51 Japanese patients with type 2 who underwent intensive including educational hospitalization regular outpatient treatment for 6 months. Dental prophylaxis without subgingival scaling was provided three times during observational period. Associations between changes in parameters glycaemic control levels were evaluated using multiple...
The search for novel enzymes and enzymatic activities is important to map out all metabolic reveal cellular processes in a more exhaustive manner. Here we present biochemical physiological evidence the function of uncharacterized protein YihU Escherichia coli using metabolite profiling by capillary electrophoresis time-of-flight mass spectrometry. To detect activity simultaneously identify possible substrates products putative enzyme, profiled complex mixture metabolites presence or absence...
Unlike bacteria such as Escherichia coli and Bacillus subtilis, several species of freshwater cyanobacteria are known to contain multiple chromosomal copies per cell, at all stages their cell cycle. We have characterized the replication multi-copy chromosomes in cyanobacterium Synechococcus elongatus PCC 7942 (hereafter 7942). In 7942, chromosome is asynchronous, not only among cells but also chromosomes. This suggests that DNA tightly coupled division 7942. To address this hypothesis, we...
Few studies have reported on the impact of oxidative stress dental implant failure. The aim this study was to investigate hyperglycemia-induced osseointegration in diabetes mellitus (DM).Acid-treated titanium implants were bilaterally placed maxillary alveolar ridge streptozotocin-induced diabetic (DM group) and control rats after extraction first molars. Histological analysis micro-push-out test performed 4 weeks surgery. Oxidative osteogenic markers surrounding bone quantified by real-time...
<title>Abstract</title> Objectives Obesity is a risk factor for periodontal disease and associated with socioeconomic status (SES). However, it remains unclear whether SES modifies the relationship between obesity disease. This study aimed to investigate influence of on association Material Methods We used multilevel Poisson regression, adjusted SES, analyze body mass index (BMI) parameters 962 participants (mean age 58.3 years; SD: 13.8). was assessed based average income education levels...
The search for novel enzymes is an important but difficult task in functional genomics. Here, we present a systematic method based on vitro assays combination with metabolite profiling to discover enzymatic activities. A complex mixture of metabolites incubated purified candidate proteins and the reaction subsequently profiled by capillary electrophoresis electrospray ionization mass spectrometry (CE−MS). Specific changes composition can directly suggest presence activity while subsequent...
The gene ybhA of Escherichia coli encodes a phosphatase that has an in vitro specificity to dephosphorylate pyridoxal 5ʹ-phosphate (PLP or vitamin B6), co-factor for aminotransferases and other enzymes. In this study, we found excess (PL) minimal medium resulted PLP vivo growth inhibition, which was alleviated by YbhA overproduction. Conversely, the overproduction shortage correlated reduction rate, significantly negated PL medium. addition, kinase, PdxK PdxY, inhibitory cell only absence...
Introduction The aim was to investigate the relationship of full-mouth inflammatory parameters periodontal disease with diabetes and obesity. Research design methods This cross-sectional study conducted diabetes-related examinations calculated inflamed epithelial surface area (PISA PESA) 71 Japanese patients type 2 diabetes. Multiple linear regression analyses were performed evaluate associations between PISA or PESA obesity parameters. Results Median value body mass index (BMI), hemoglobin...
Mycinamicin, composed of a branched lactone and two sugars, desosamine mycinose, at the C-5 C-21 positions, is 16-membered macrolide antibiotic produced by Micromonospora griseorubida A11725, which shows strong antimicrobial activity against Gram-positive bacteria. The nucleotide sequence (62 kb) mycinamicin biosynthetic gene cluster, in there were 22 open reading frames (ORFs), was completely determined. All products from ORFs are responsible for biosynthesis II self-protection compounds...
Leucine-responsive regulatory protein (Lrp) is a transcriptional regulator for the genes involved in transport, biosynthesis and catabolism of amino acids Escherichia coli . In order to identify whole set under direct control Lrp, we performed Genomic SELEX screening identified total 314 Lrp-binding sites on E. genome. As result, regulation target Lrp was predicted expand from hitherto acid metabolism novel utilization synthesis, including tRNAs, aminoacyl-tRNA synthases rRNAs. Northern blot...
Abstract Malnutrition-inflammation-atherosclerosis (MIA) syndrome is a significant risk factor for mortality in patients undergoing hemodialysis. This study aimed to investigate the association between MIA and oral health status hemodialysis patients. A cross-sectional was conducted on 254 Comprehensive medical dental examinations were performed. Three components included define syndrome: Geriatric Nutritional Risk Index, serum high-sensitivity C-reactive protein, history of cardiovascular...
ABSTRACT Aim Traditional Japanese (Kampo) medicine has been used to treat viral infectious diseases. In particular, saikatsugekito (a combination drug of kakkonto, shosaikoto, Platicodon glandiflorum root, and gypsum) reported be useful during the past influenza pandemic. The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spread worldwide, causing novel disease (COVID‐19) emerge as a this article, we conducted literature review on pharmacological activities components present...
This study is focused on the involvement of unusual nucleotide (p)ppGpp during morphological and physiological differentiation Streptomyces clavuligerus. In particular, functional structural elements two genes encoding proteins RelA Rsh were identified. The relA gene encodes an 843 aa protein (RelA), while rsh a 738 (Rsh). disrupted by insertion hygromycin resistance apramycin gene, respectively. synthesis ppGpp in gene-disrupted mutant was completely eliminated under conditions starvation...
Disruption of eshA, which encodes a 52-kDa protein that is produced late during the growth Streptomyces coelicolor A3(2), resulted in elimination actinorhodin production. In contrast, disruption eshB, close homologue had no effect on antibiotic The eshA disruptant accumulated lower levels ppGpp than wild-type strain accumulated. loss production was restored by expression truncated relA gene, increased level to strain, indicating reduced accumulation mutant solely responsible for Antibiotic...
Acetate overflow refers to the metabolism by which a large part of carbon incorporated as glucose into Escherichia coli cells is catabolized and excreted acetate medium. We previously found that mutants for pathway enzymes phosphoacetyltransferase (Pta) kinase (AckA) showed significant diauxic growth after depletion in E. . Here, we analyzed underlying mechanism pta mutant. Proteomic other analyses revealed an increase pyruvate dehydrogenase complex subunits decrease glyoxylate shunt...
This study aimed to investigate the effects of metformin on gingival wound healing in insulin-resistant prediabetes.C57BL/6J mice were fed normal diet (ND) or high-fat (HFD) for 10 weeks; half HFD treated with (HFD+ Met) last 2 weeks. Insulin and glucose tolerance tests performed. The palatal gingiva (2.0 × 0.5 mm) was surgically removed adjacent maxillary molars. Post-surgical closure histomorphometrically evaluated 1 week. mRNA expression vascular endothelial growth factor (VEGF) nitric...
We have performed a screening of hydroxyurea (HU)-sensitive mutants using single-gene-deletion mutant collection in Escherichia coli K-12. HU inhibits ribonucleotide reductase (RNR), an enzyme that catalyzes the formation deoxyribonucleotides. Unexpectedly, seven lacked genes are required for incorporation sulfur into specific tRNA modification base, 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U), via persulfide relay. found expression RNR was reduced to about one-third both absence and...