A5023140770- Food composition and properties
- Plant Reproductive Biology
- Phytase and its Applications
- GABA and Rice Research
- Rice Cultivation and Yield Improvement
- Wheat and Barley Genetics and Pathology
- Proteins in Food Systems
- Biofuel production and bioconversion
- Endoplasmic Reticulum Stress and Disease
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- RNA Research and Splicing
- Genetics and Plant Breeding
- Enzyme Production and Characterization
- Soybean genetics and cultivation
- Plant pathogens and resistance mechanisms
- Cellular transport and secretion
- RNA regulation and disease
- Plant Gene Expression Analysis
- Polysaccharides and Plant Cell Walls
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
Kyushu University
2014-2023
Agricultural Research Center
2023
Genetic Resources Center
2016-2023
Field Crops Research Institute
2023
Damanhour University
2023
Cambridge University Press
2018
New York University Press
2018
Vietnam Academy of Agricultural Sciences
2013
Yamaguchi Prefectural University
2004-2011
Washington State University
2004-2011
Inflorescence structures result from the activities of meristems, which coordinate both renewal stem cells in center and organ formation at periphery. The fate a meristem is specified its initiation changes as plant develops. During rice inflorescence development, newly formed meristems acquire branch (BM) identity, can generate further or terminate spikelets. Thus, form determined by reiterative pattern decisions made meristems. In dominant gain-of-function mutant tawawa1-D , activity (IM)...
Abstract Rice (Oryza sativa) accumulates prolamins and glutelins as storage proteins. The latter protein is synthesized on the endoplasmic reticulum (ER) a 57-kD proglutelin precursor, which then processed into acidic basic subunits in vacuole. Three esp2mutants, CM1787, EM44, EM747, contain larger amounts of polypeptide corresponding lower levels glutelin than normal. Electron microscopic observation revealed thatesp2 contained normal-appearing glutelin-containing bodies (PB-II), but lacked...
Protein body type one (PB-I) was isolated and purified from developing rice grain by a combination of sucrose density gradient centrifugation treatment with pepsin. SDS-PAGE analysis showed that PB-I contains several polypeptide groups, the largest having an apparent molecular size 13 kDa two smaller ones 10 16 kDa. The 13-kDa group found to be composed polypeptides slightly different sizes, 13a (larger component) 13b (smaller component). Most were shown largely prolamins, although there...
In rice (Oryza sativa L.), leaf photosynthesis is known to be highly correlated with stomatal conductance; however, it remains unclear whether conductance dominantly limits the photosynthetic rate. SLAC1 a anion channel protein controlling closure in response environmental [CO2]. order examine limitations photosynthesis, SLAC1-deficient mutant of was isolated and characterized. A TILLING screen N-methyl-N-nitrosourea-derived lines conducted for orthologue gene Os04g0674700, four containing...
Axillary shoot formation is a key determinant of plant architecture. Formation the axillary regulated by initiation meristem or outgrowth bud. Here, we show that rice (Oryza sativa) TILLERS ABSENT1 (TAB1; also known as Os WUS), an ortholog Arabidopsis thaliana WUS, required to initiate development. We found in proceeds via transient state, which term premeristem, characterized expression OSH1, marker indeterminate cells apical meristem. In tab1-1 (wus-1) mutant, however, arrested at various...
Starch granule morphology differs markedly among plant species. However, the mechanisms controlling starch have not been elucidated. Rice (Oryza sativa) endosperm produces characteristic compound-type granules containing dozens of polyhedral within an amyloplast. Some other cereal species produce simple-type granules, in which only one is present per A double mutant rice deficient synthase (SS) genes SSIIIa and SSIVb (ss3a ss4b) produced spherical whereas parental single mutants similar to...
The developing endosperm of rice (Oryza sativa, Os) synthesizes a large amount storage proteins on the rough (r)ER. major proteins, glutelins and prolamins, contain either intra or intermolecular disulfide bonds, oxidative protein folding is necessary for sorting to bodies. Here, we investigated an electron transfer pathway formation bonds in rER endosperm, focusing roles thiol-disulfide oxidoreductase, OsEro1. Confocal microscopic analysis revealed that N-glycosylated OsEro1 localized...
Abstract In the rice (Oryza sativa) endosperm, storage proteins are synthesized on rough endoplasmic reticulum (ER), in which prolamins sorted to protein bodies (PBs) called type-I PB (PB-I). Protein disulfide isomerase (PDI) family oxidoreductase PDIL2;3, an ortholog of human P5, contains a conserved structural redox-inactive thioredoxin-like (TRX) domain and was efficiently targeted surface PB-I redox active site–dependent manner, whereas PDIL1;1, PDI, localized ER lumen. Complementation...
The gelatinization temperature of endosperm starch in most japonica rice cultivars is significantly lower than that indica cultivars. This because three single nucleotide polymorphisms the Starch synthase (SS) IIa gene (SSIIaJ) reduce SSIIa activity, resulting an increase amylopectin short chains with degree polymerization (DP)12 compared to (SSIIaI). forms a trimeric complex SSI and branching enzyme (BE) IIb maize rice, which likely important for biosynthesis intermediate (DP≤24) within...
To identify the function of genes that regulate processing proglutelin, we performed an analysis glup3 mutants, which accumulates excess amounts proglutelin and lack vacuolar enzyme (VPE). VPE activity in developing seeds from lines was reduced remarkably compared with wild type. DNA sequencing gene mutants revealed either amino acid substitutions or appearance a stop codon within coding region. Microscopic observations showed α-globulin were distributed homogeneously protein storage...
The rice esp2 mutation was previously characterized by the abnormal accumulation of elevated levels proglutelin and absence an endosperm-specific protein disulfide isomerase like (PDIL1-1). Here we show that Esp2 is structural gene for PDIL1-1 this lumenal chaperone asymmetrically distributed within cortical endoplasmic reticulum (ER) largely restricted to cisternal ER. Temporal studies indicate essential maturation only when its rate synthesis significantly exceeds export from ER, a...
The rice prolamins consist of cysteine-rich 10 kDa (CysR10), 14 (CysR14) and 16 (CysR16) molecular species a cysteine-poor 13 (CysP13) polypeptide. These storage proteins form protein bodies (PBs) composed single spherical intracisternal inclusions assembled within the lumen rough endoplasmic reticulum. Immunofluorescence immunoelectron microscopy demonstrated that CysR10 CysP13 were asymmetrically distributed PBs, with former concentrated at electron-dense center core region latter mainly...
Rice seed storage proteins glutelin and α-globulin are synthesized in the endoplasmic reticulum (ER) deposited protein vacuoles (PSVs). Sar1, a small GTPase, acts as molecular switch to regulate assembly of coat complex II, which exports secretory from ER Golgi apparatus. To reveal route by exit ER, four putative Sar1 genes (OsSar1a/b/c/d) were cloned rice, transgenic rice generated with overexpressed or suppressed RNA interference (RNAi) specifically endosperm under control promoter....
Abstract Rice (Oryza sativa) glutelins are synthesized on the endoplasmic reticulum as a precursor, which then transported via Golgi to protein storage vacuoles (PSVs), where they proteolytically processed into acidic and basic subunits. The glutelin precursor mutant6 (glup6) accumulates abnormally large amounts of proglutelin. Map-base cloning studies showed that glup6 was loss-of-function mutant guanine nucleotide exchange factor (GEF), activates Rab GTPase, key regulator membrane...
In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported anchored to distinct subdomains cortical endoplasmic reticulum. RNA binding protein RBP-P binds both prolamine mRNAs, suggesting a role in some aspect their metabolism. Here, we show that lines expressing mutant mislocalize mRNAs. Different proteins exhibited varying degrees reduced and/or protein-protein interaction properties, which may account for mislocalization RNAs....
Two mutants for rice storage protein in the starchy endosperm, 13b-L and 57-H, were crossed with their original variety, Kinmaze. From results obtained F1 F2 seeds, it was concluded that 57-H are controlled by single recessive genes, which designated as esp-1 esp-2, respectively. The SDS-PAGE indicates both mutant genes regulatory bodies. segregation ratio of cross between trisomic type F esp-2 G fitted a segregation. These suggest located on chromosomes 10 9,
The efficiency of N-methyl-N-nitrosourea (MNU) treatment fertilized egg cells at the single-cell stage in rice (Oryza sativa L.) was about twice that dry seeds. proportion recessive iso-chlorophyll mutants segregating progeny M1 plants decreased remarkably with later stages embryonic development, perhaps because presence diplontic selection and chimera formation when older, multicellular embryos were mutagenized. Panicle sterility showed a linear relationship frequency chlorophyll mutations...
Abstract Rice (Oryza sativa) glutelins are synthesized on the endoplasmic reticulum as larger precursors, which then transported via Golgi to protein storage vacuole (PSV), where they processed into acidic and basic subunits. Three independent glutelin precursor mutant4 (glup4) rice lines, accumulated elevated levels of proglutelin over wild type, were identified loss-of-function mutants Rab5a, small GTPase involved in vesicular membrane transport. In addition plasma membrane, Rab5a...
Studies focusing on the targeting of RNAs that encode rice storage proteins, prolamines and glutelins to specific sub-domains endoplasmic reticulum (ER), as well mis-localization studies other protein RNAs, indicate a close relationship between ER site RNA translation final deposition in endomembrane system developing endosperm. In addition prolamine glutelin, accumulates smaller amounts α-globulins, which are deposited together with glutelin vacuole (PSV). situ RT-PCR analysis revealed...