A5061728737- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Caveolin-1 and cellular processes
- Lipid Membrane Structure and Behavior
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Force Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Cell Adhesion Molecules Research
- Neurofibromatosis and Schwannoma Cases
- Renal and related cancers
- Skin and Cellular Biology Research
- Advanced Fluorescence Microscopy Techniques
- Wnt/β-catenin signaling in development and cancer
- Bacteriophages and microbial interactions
- Signaling Pathways in Disease
- Hippo pathway signaling and YAP/TAZ
- Connective tissue disorders research
- Photosynthetic Processes and Mechanisms
- Metal Forming Simulation Techniques
- Ion Channels and Receptors
- Cholesterol and Lipid Metabolism
- Hemoglobin structure and function
Okayama University
2020-2024
Okayama University of Science
2023-2024
University of Helsinki
2014-2023
Czech Academy of Sciences, Institute of Biotechnology
2015-2018
The University of Tokyo
2009-2015
Helsinki Institute of Physics
2015
Tohoku University
2006-2008
Actin-depolymerizing factor (ADF)/cofilins contribute to cytoskeletal dynamics by promoting rapid actin filament disassembly. In the classical view, ADF/cofilin sever filaments, and capping proteins block barbed ends whereas pointed depolymerize, at a rate that is still debated. Here, monitoring activity of three mammalian isoforms on individual skeletal muscle cytoplasmic we directly quantify reactions underpinning severing depolymerization from both ends. We find that, in absence monomeric...
Caveolae are flask-shaped invaginations of the plasma membrane that associated with tumor formation, pathogen entry and muscular dystrophy, through regulation lipids, signal transduction endocytosis. generated by fusion caveolin-1-containing vesicles membrane, which then participate in endocytosis via dynamin. Proteins containing membrane-sculpting F-BAR (or EFC) domains organize clathrin-mediated Here, we show protein PACSIN2 sculpts caveola. The domain interacts directly caveolin-1...
Significance Membrane phosphoinositides have emerged as key regulators of the actin cytoskeleton in cell migration, morphogenesis, cytokinesis, and endocytosis. However, molecular mechanisms by which actin-binding proteins (ABPs) interact with phosphoinositide-rich membranes remain remarkably poorly understood. By applying a combination biochemical, biophysical, atomistic dynamics simulation approaches on six central ABPs, we discovered that they employ multivalent electrostatic interactions...
One challenge in cell biology is to decipher the biophysical mechanisms governing protein enrichment on curved membranes and resulting membrane deformation. The ERM ezrin abundant associated with cellular that are flat, positively or negatively curved. Using vitro approaches, we assess of ezrin’s membranes. We evidence wild-type (ezrinWT) its phosphomimetic mutant T567D (ezrinTD) do not deform but self-assemble anti-parallelly, zipping adjacent EzrinTD’s specific conformation reduces...
Filopodia are actin-rich membrane protrusions essential for cell morphogenesis, motility, and cancer invasion. How cells control filopodium initiation on the plasma remains elusive. We performed experiments in cellulo, vitro, silico to unravel mechanism of driven by curvature sensor IRSp53 (insulin receptor substrate protein 53 kDa). showed that full-length self-assembles into clusters membranes depending PIP2. Using well-controlled vitro reconstitution systems, we demonstrated recruit actin...
Abstract Membrane-enclosed organelles are defining features of eukaryotes in distinguishing these organisms from prokaryotes. Specification distinct membranes is critical to assemble and maintain discrete compartments. Small GTPases their regulators the signaling molecules that drive membrane-modifying machineries desired location. These include Rab Rag GTPases, roadblock longin domain proteins, TRAPPC3-like proteins. Here, we take a structural approach assess relatedness eukaryotic-like...
PACSIN2, a membrane-sculpting BAR domain protein, localizes to caveolae. Here, we found that PKC phosphorylates PACSIN2 at serine 313, thereby decreasing its membrane binding and tubulation capacities. Concomitantly, phosphorylation decreased the time span for which caveolae could be tracked plasma (the 'tracking-duration'). Analyses of phospho-mimetic S313E mutant suggested is sufficient reduce caveolar tracking-durations. Both hypotonic treatment isotonic drug-induced activation increased...
Abstract Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, conducting high-resolution microscopy approaches we unveil the presence an actomyosin cable encircling TEMs. We develop theoretical cellular framework interpret physical...
We studied the process of formation stress fibres and involvement phosphorylation myosin-II during spreading Swiss 3T3 fibroblasts. In cells that were allowed to spread for 1 h on a glass surface, circular bundles actin filament present. At 2–3 after plating, showed polygonal polarized shape. The proportion having was decreased, whereas with straight filaments increased. 4 completely present at periphery dorsal ventral surfaces cells. Thus, possessed different forms actomyosin corresponding...
Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated extent caveolar by measuring density projected onto a two-dimensional (2D) plane. The in flattened is assumed to one-quarter caveola. proportion one-quarter-density increased after increasing hypo-osmotic...
Podosomes are cellular "feet," characterized by F-actin-rich membrane protrusions, which drive cell migration and invasion into the extracellular matrix. Small GTPases that regulate actin cytoskeleton, such as Cdc42 Rac central regulators of podosome formation. The adaptor protein IRSp53 contains an I-BAR domain deforms membranes protrusions binds to Rac, a CRIB motif interacts with Cdc42, SH3 many cytoskeletal proline-rich peptides including VASP, C-terminal variable region splicing....
Metastasis-suppressor 1 (MTSS1) is a membrane-interacting scaffolding protein that regulates the integrity of epithelial cell-cell junctions and functions as tumor suppressor in wide range carcinomas. MTSS1 binds phosphoinositide-rich membranes through its I-BAR domain capable sensing generating negative membrane curvature vitro. However, mechanisms by which localizes to intercellular cells contributes their maintenance have remained elusive. By carrying out EM live-cell imaging on cultured...
Abstract Charting the emergence of eukaryotic traits is important for understanding characteristics organisms that contributed to eukaryogenesis. Asgard archaea and eukaryotes are only known possess regulated actin cytoskeletons. Here, we determined gelsolins (2DGels) from Lokiarchaeota (Loki) Heimdallarchaeota (Heim) capable regulating dynamics in vitro when expressed cells. The filament severing capping, monomer sequestering, functionalities 2DGels strictly calcium controlled. We X-ray...
Primary cilia are sensory organelles crucial for organ development. The pivotal structure of the primary is a microtubule that generated via tubulin polymerization reaction occurs in basal body. It remains to be elucidated how molecules with distinct physicochemical properties contribute formation cilia.Here we show brain expressed X-linked 1 (Bex1) plays an essential role and formation. Bex1 protein shows property being intrinsically disordered (IDP). cell density-dependent accumulation as...