A5039442964- RNA Research and Splicing
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Biotin and Related Studies
- Protein Structure and Dynamics
- Neuroscience and Neuropharmacology Research
- Genetic Neurodegenerative Diseases
- Lipid Membrane Structure and Behavior
- Cancer-related Molecular Pathways
- Pharmacological Effects of Natural Compounds
- Endoplasmic Reticulum Stress and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- Enzyme Structure and Function
- Click Chemistry and Applications
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Genomics and Chromatin Dynamics
- Folate and B Vitamins Research
- Ubiquitin and proteasome pathways
- Cholesterol and Lipid Metabolism
- Animal Vocal Communication and Behavior
- Phagocytosis and Immune Regulation
- Neurotransmitter Receptor Influence on Behavior
- Animal Behavior and Reproduction
Nagoya University
2020-2023
Shiga University of Medical Science
2019-2022
Shiga University
2019-2021
Kyoto University
2007-2017
Gifu University
2006-2008
The expression of eukaryotic genes is precisely controlled by specific interactions between general transcription initiation factors and gene-specific transcriptional activators. factor TFIID, which plays an essential role in mediating activation, a multisubunit complex comprising the TATA box-binding protein (TBP) multiple TBP-associated (TAFs). On other hand, biochemical genetic approaches have shown that promoter-specific activator Sp1 has ability to interact with one components TAF4. We...
The expression of eukaryotic genes is precisely controlled by interactions between general transcriptional factors and promoter-specific activators. fourth element TATA-box binding protein-associated factor (TAF4), an essential subunit the transcription TFIID, serves as a coactivator for various regulators. Interactions TAF4 site-specific activators, such Sp1, are important regulating levels interest. However, only limited information available on molecular mechanisms underlying these...
Primary cilia are sensory organelles crucial for organ development. The pivotal structure of the primary is a microtubule that generated via tubulin polymerization reaction occurs in basal body. It remains to be elucidated how molecules with distinct physicochemical properties contribute formation cilia.Here we show brain expressed X-linked 1 (Bex1) plays an essential role and formation. Bex1 protein shows property being intrinsically disordered (IDP). cell density-dependent accumulation as...
The promoter-specific transcription factor Sp1 is expressed ubiquitously, and plays a primary role in the regulation of expression many genes. Domains A B located N-terminal half protein are characterized by glutamine-rich (Q-rich) sequences. These Q-rich domains have been shown to be involved interaction between different classes nuclear proteins, such as TATA-binding associated factors. Furthermore, self-association via also important for transcriptional activity. It has considered that an...
Background: Brain amyloid-β (Aβ) peptide is released into the interstitial fluid (ISF) in a neuronal activity-dependent manner, and Aβ deposition Alzheimer’s disease (AD) linked to baseline activity. Although intrinsic mechanism for generation remains be elucidated, interleukin-like epithelial-mesenchymal transition inducer (ILEI) candidate an endogenous suppressor. Objective: This study aimed access underlying ILEI secretion its effect on production brain. Methods: levels cerebral cortex...
Glycyrrhizin (GL) is known to exhibit a variety of useful pharmacological activities, including anti-inflammation, anti-hepatotoxicity, and enhancement intestinal drug absorption. GL has been reported modify the assembly actin filaments, thereby modulating tight junction (TJ) integrity, but detailed molecular mechanisms this remain unclear. In study, we first found that binds PDZ domain zonula occludens-1 (ZO-1(PDZ1)) through NMR experiments. The structure GL-ZO-1(PDZ1) complex was then...
The anti-oncogenic protein p53 is a transcription factor that prevents tumorigenesis by inducing gene repair proteins or apoptosis under DNA damage. Since the DNA-binding domain of (p53C) aggregation-prone, function often lost in cancer cells. This tendency rather severe some tumor-related mutants, such as R175H. In this study, we examined effect salts, including KCl and sugars, on aggregation p53C monitoring two distinct aggregates: amorphous-like amyloid-like. amorphous aggregates are...
Perturbation of the homeostasis brain membrane lipids has been implicated in pathomechanism Alzheimer's disease (AD). The ε4 allele apolipoprotein E gene (APOE) confers an increased risk, a dosage-dependent manner, for amyloid-β accumulation and development sporadic AD. An effect APOE genotype on lipid may underlie AD risk associated with allele. In this research, we examined class composition crude membranes raft-enriched fractions brains. We applied enzymatic reaction-based methods...
The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use recombinant proteins with fusion-protein tags, such as maltose-binding protein (MBP) glutathione-S-transferase (GST). In this study, we improved cohesive sticky properties gelatinized starch by supplementing it agarose, resulting in harder gel could coat bottom microtiter plate. starch/agarose mixture allowed for efficient immobilization MBP-tagged on coated...
Human stomatin (hSTOM) is a component of the membrane skeleton erythrocytes that maintains membrane's shape and stiffness through interconnecting spectrin actin. hSTOM member protein family possesses single stomatin/prohibitin/flotillin/HflK (SPFH) domain at center molecule. Although SPFH proteins are widely distributed from archaea to mammals, detailed function remains unclear. In this study, we first determined solution structure (hSTOM(SPFH)) via NMR. The hSTOM(SPFH) essentially identical...
Continuous-flow FRET is a powerful technique to obtain site-specific information on tertiary structure formation during early stages of protein folding.We studied the chain condensation within beta-barrel domain and between alpha-helical domains SNase folding.Variants with single pair in showed an increase efficiency sub-millisecond time scale.In contrast, variant donor acceptor only after 100 ms folding.These results indicate that betabarrel adopts compact folding while forms final stages.
The detection and quantification of protein-protein interactions (PPIs) is a crucial technique that often involves the use recombinant proteins with fusion protein tags, such as maltose-binding (MBP) glutathione-S-transferase (GST). In this study, we improved cohesive sticky properties gelatinized starch by supplementing it agarose, resulting in harder gel could coat bottom microtiter plate. starch/agarose mixture allowed for efficient immobilization MBP-tagged on coated plates, enabling...