A5001869190- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Ion channel regulation and function
- Spectroscopy and Quantum Chemical Studies
- Chemical Synthesis and Analysis
- Erythrocyte Function and Pathophysiology
- Supramolecular Self-Assembly in Materials
- Electromagnetic Fields and Biological Effects
- Electrochemical Analysis and Applications
- Molecular Sensors and Ion Detection
- Analytical Chemistry and Sensors
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Antimicrobial agents and applications
- Advanced biosensing and bioanalysis techniques
- Biochemical and Structural Characterization
- Optical Coherence Tomography Applications
- Molecular Junctions and Nanostructures
- Air Quality and Health Impacts
- Plant Stress Responses and Tolerance
- Microbial Inactivation Methods
- Gold and Silver Nanoparticles Synthesis and Applications
- Luminescence and Fluorescent Materials
- Magnetic and Electromagnetic Effects
- Ion Transport and Channel Regulation
University of Technology Sydney
2016-2025
ARC Centre of Excellence in Future Low-Energy Electronics Technologies
2020-2021
Victor Chang Cardiac Research Institute
2000-2014
UNSW Sydney
2000-2014
St Vincent's Clinic
2012-2013
Jena University Hospital
2009-2011
Swinburne University of Technology
2001-2007
Keele University
2003-2004
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in protection bacterial cells against hypoosmotic shock. Although a great deal is known about structure function these channels, much less how membrane lipids may influence their mechanosensitivity function. In this study, we use liposome coreconstitution to examine effects different types on MscS MscL simultaneously using patch-clamp technique confocal microscopy. Fluorescence lifetime imaging...
Electronic cigarette (e-cigarette) use is on the rise worldwide and particularly attractive to young people as a smoking substitute by pregnant woman. There perception in women of child-bearing age that e-cigarettes (vaping) safer than tobacco cigarettes during pregnancy. However, there little evidence support this perception. Here, we examined offspring from mouse dams had been exposed after pregnancy ambient air (sham) (n = 8), e-cigarette aerosols with nicotine or without 8). Offspring...
Two-photon fluorescence and second harmonic generation microscopy have enabled functional morphological in vivo imaging. However, applications of those techniques to living animals are limited by bulk optics on a bench top. Fortunately, growing functionality fiber-optic devices miniaturization scanning mirrors stimulate the race develop nonlinear optical endoscopy. In this paper, we report prototype endoscope based double-clad photonic crystal fiber improve detection efficiency MEMS mirror...
Antimicrobial resistance in bacteria is becoming increasingly prevalent, posing a critical challenge to global health. Bacterial biofilm formation common mechanism that reduces the effectiveness of antibiotics. Thus, development compounds can disrupt bacterial biofilms potential strategy combat antimicrobial resistance. We report herein synthesis amphipathic guanidine-embedded glyoxamide-based peptidomimetics via ring-opening reactions N-naphthoylisatins with amines and amino acids. These...
Understanding membrane transport processes such as ion occlusion reactions of pumps and transporters the gating channels requires knowledge lipid bilayer electrostatics. A simple example effect electrostatics on is much higher permeability to hydrophobic anions, tetraphenylborate (TPB-), compared cations, tetraphenylphosphonium (TPP+) or tetraphenylarsonium (TPA+). This has been attributed dipole potential, which a major contributor determined be oriented water dipoles in headgroup region...
This study explains the importance of phosphate moiety and H3O+ in controlling ionic flux through phospholipid membranes. We show that despite an increase concentration when pH is decreased, level conduction bilayers reduced. By modifying lipid structure, we dominant determinant membrane hydrogen bonding between oxygens on adjacent phospholipids. The modulation with proposed to arise from varying concentrations altering molecular area per geometry conductive defects already present membrane....
Cyclotides are cyclic disulfide-rich peptides that chemically and thermally stable possess pharmaceutical insecticidal properties. The activities reported for cyclotides correlate with their ability to target phosphatidylethanolamine (PE)-phospholipids disrupt cell membranes. However, the mechanism by which this disruption occurs remains unclear. In current study we examine effect of prototypic cyclotides, kalata B1 (kB1) B2 (kB2), on tethered lipid bilayer membranes (tBLMs) using swept...
The synthesis of novel glyoxamide-based antimicrobial peptide mimics is described.
Abstract Mutation, irregular expression and sustained activation of the Transient Receptor Potential Channel, type Melastatin 4 (TRPM4), have been linked to various cardiovascular diseases. However, much remains unknown about structure this important ion channel. Here, we purified a heterologously expressed TRPM4-eGFP fusion protein investigated oligomeric state in detergent micelles using crosslinking, native gel electrophoresis, multi-angle laser light scattering electron microscopy. Our...
Melimine and its derivatives are synthetic chimeric antimicrobial agents based on protamine melittin. The binding of solubilized melimine derivative, with a cysteine N-terminus, (cys-melimine) tethered bilayer lipid membranes (tBLMs) was examined using ac electrical impedance spectroscopy. addition cys-melimine initially increased membrane conduction, which subsequently falls over time. results were obtained for tBLMs comprising zwitterionic phosphatidylcholine, anionic phosphatidylglycerol,...
This work seeks to identify the mechanisms by which hydronium ions (H3O+) modulate structure of phospholipid bilayers studying interactions H3O+ with phospholipids at molecular level. For this, we carried out multiple microsecond-long unrestrained dynamics (MD) simulations a POPC bilayer different concentrations. The results show that accumulates membrane surface where it displaces water and forms strong long-lived hydrogen bonds phosphate carbonyl oxygens in phospholipids....
There are numerous structural and cultural barriers to the progression of women marginalized groups leadership in academia, especially Science, Technology, Engineering, Mathematics Medicine (STEMM). A range interventions have been described address this inequity, with varying success. Here, we suggest that sponsorship could be one effective intervention propose an institutional action plan implement a program academia. We outline why strategy, if implemented through deliberate by...
Abstract Maternal smoking is currently a public health concern and has been associated with number of complications in the offspring. E-cigarettes are gaining popularity as “safer” alternative to tobacco cigarettes during pregnancy, however, there limited studies suggest that it actually “safe.” Balb/C female mice were exposed ambient air (n = 8; Sham), or cigarette smoke SE) before gestation, gestation lactation. A third group was followed by e-cigarette aerosols lactation Switch). Male...