A5028359964- Antimicrobial Peptides and Activities
- Radioactivity and Radon Measurements
- Antimicrobial agents and applications
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Nuclear and radioactivity studies
- Bacterial biofilms and quorum sensing
- Graphite, nuclear technology, radiation studies
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Polydiacetylene-based materials and applications
- Corneal Surgery and Treatments
- Radioactive contamination and transfer
- Immune Response and Inflammation
- Bone Tissue Engineering Materials
- Radiation Shielding Materials Analysis
- Antibiotic Use and Resistance
- Heavy metals in environment
- Indoor Air Quality and Microbial Exposure
- Dental materials and restorations
- Infection Control in Healthcare
- Graphene and Nanomaterials Applications
- Infection Control and Ventilation
- Belt and Road Initiative
- South Asian Studies and Conflicts
UNSW Sydney
2018-2025
Hackensack University Medical Center
2024
University of Engineering and Technology Lahore
2024
University of Haripur
2021-2023
University of Balochistan
2022
University of Peshawar
2021
University of Chinese Academy of Sciences
2021
Bahawal Victoria Hospital
2018-2021
Institute of Geochemistry
2021
Hamad Medical Corporation
2021
Abstract Melimine and Mel4 are chimeric cationic peptides with broad-spectrum antimicrobial activity. They have been shown to be highly biocompatible in animal models human clinical trials. The current study examined the mechanism of action these two against P. aeruginosa . effect endotoxin neutralization, their interactions cytoplasmic membranes using DiSC(3)-5 Sytox green, Syto-9 PI dyes were analysed. Release ATP DNA/RNA determined luminescence increase OD 260 nm bacteriolytic ability was...
Mel4 is a novel cationic peptide with potent activity against Gram-positive bacteria. The current study examined the anti-staphylococcal mechanism of action and its precursor melimine. interaction peptides lipoteichoic acid (LTA) cytoplasmic membrane using DiSC(3)-5, Sytox green, Syto-9 PI dyes were studied. Release ATP DNA/RNA from cells exposed to determined. Bacteriolysis autolysin-activated cell death determined by measuring decreases in OD620nm killing Micrococcus lysodeikticus...
Bacterial colonization and biofilm development on medical devices can lead to infection. Antimicrobial peptide-coated surfaces may prevent such infections. Melimine Mel4 are chimeric cationic peptides showing broad-spectrum antimicrobial activity once attached biomaterials highly biocompatible in animal models have been tested Phase I II/III human clinical trials. These were covalently glass using an azidobenzoic acid linker. Peptide attachment was confirmed X-ray photoelectron spectroscopy...
Protection against pathogens using personal protective equipment is essential yet challenging in healthcare settings. Concerns over emerging biothreats and outbreaks of infectious diseases underscore the need for antimicrobial biocompatible clothing to protect patients staff. Herein, we report efficacy cytotoxicity cotton/silk fabrics containing embedded reduced graphene oxide (RGO) Ag/Cu nanoparticles (NPs), prepared a 3-glycidyloxypropyl trimethoxy silane coupling agent followed by...
Even with the best infection control protocols in place, risk of a hospital-acquired surface an implanted device remains significant. A bacterial biofilm can form and has potential to escape host immune system develop resistance conventional antibiotics, ultimately causing implant fail, seriously impacting patient well-being. Here, we demonstrate 4 log reduction rate by common pathogen S. aureus 3D-printed polyaryl ether ketone (PAEK) polymeric surfaces covalently binding antimicrobial...
Pseudomonas aeruginosa is increasingly resistant to conventional antibiotics, which can be compounded by the formation of biofilms on surfaces conferring additional resistance. P. was grown in sub-inhibitory concentrations antimicrobial peptides (AMPs) melimine and Mel4 or ciprofloxacin for 30 consecutive days induce development Antibiofilm effect AMPs evaluated using crystal violet live/dead staining with confocal microscopy. Effect cell membrane biofilm cells DiSC(3)-5 dye release...
Periodic surveillance of antibiotic consumption in the form point prevalence studies is a quick and robust methodology to evaluate prescribing trends hospitals. The current study was undertaken document among neonates children from hospitals Pakistan.This large multicenter using World Health Organization standardized AWaRe (Access, Watch, Reserve) classification examined for suspected bacterial infection admitted Punjab, Pakistan.A total 708 beds wards 16 health facilities were examined....
The presence of pathogenic bacteria in food is considered as a primary cause food-borne illness and quality deterioration worldwide. present study aimed to determine the effectiveness five essential oils (EOs) against multidrug-resistant foodborne pathogens. In current Gram-negative (Escherichia, Enterobacter, Citrobacter, Proteus, Pseudomonas, Klebsiella) Gram-positive Staphylococcus were isolated from raw milk biochemically characterized. anti-bacterial effect different antibiotics EOs...
BackgroundOcular infections caused by antibiotic-resistant pathogens can result in partial or complete vision loss. The development of pan-resistant microbial strains poses a significant challenge for clinicians as there are limited antimicrobial options available. Synthetic peptoids, which sequence-specific oligo-N-substituted glycines, offer potential alternative agents to target multidrug-resistant bacteria.MethodsThe activity synthesised peptoids against (MDR) ocular was evaluated using...
Soft contact lenses are a common mode of vision correction used worldwide. However, lens wearers often experience discomfort and at risk developing microbial infections. To address these issues, we designed Mel4-coated hyaluronic acid-laden etafilcon to improve wettability while providing antimicrobial properties. The process involved the interaction lens, which carries net negative charge, with positively charged Mel4 (a cationic peptide). This was followed by acid negatively polymer)....
Biomaterial-associated infections are one of the major causes implant failure. These result from persistent bacteria that have adhered to biomaterial surface before, during, or after surgery and formed a biofilm on implant's surface. It is estimated 4 10% surfaces contaminated with bacteria; however, infection rate can be as high 30% in intensive care units developed countries 45% developing countries. To date, there no clinical solution prevent without relying use doses antibiotics supplied...
Bioaccumulation of toxic metals in fish causes serious threats to human when they are consumed.Thus, the detection element concentration levels aquatic component is important.The accumulation some heavy such as Cd, Cu, Fe, Mn, Pb and Zn were determined.Ten species (Selaroides leptolepis, Decapterus maraudsi, Epinephelus lanceolatus, Priacanthus tayenus, Rastrelliger, Megalaspis cordyla, Nibea soldado, Pristipomoides filamentosus, Bramidae Siganus canaliculatus) collected from Terengganu...
Staphylococcus aureus can develop resistance by mutation, transfection or biofilm formation. Resistance was induced in S. growth sub-inhibitory concentrations of ciprofloxacin for 30 days. The ability the antimicrobials to disrupt biofilms determined using crystal violet and live/dead staining. Effects on cell membranes cells were evaluated measuring release dyes ATP, nucleic acids. None strains developed AMPs while only ATCC 25923 (128 times) after passages. Only peptides reduced...
We describe a library of amphiphilic anthranilamide compounds as antimicrobial peptide (AMP) mimics.
Abstract Mel4 is a novel cationic peptide with potent activity against Gram-positive bacteria. The current study examined the anti-staphylococcal mechanism of action and its precursor melimine. interaction peptides lipoteichoic acid (LTA) cytoplasmic membrane using DiSC(3)-5, Sytox green, Syto-9 PI dyes were studied. Release ATP DNA/RNA from cells exposed to determined. Bacteriolysis autolysin-activated cell death determined by measuring decreases in OD 620nm killing Micrococcus luteus...
Catheter-associated biofilms are responsible for a large fraction of hospital acquired infections. Antimicrobial surface coating on catheters providing prevention at source is extensively studied to reduce bacterial adhesion. peptides such as melimine and Mel4, covalently linked surfaces, have shown excellent potential in animal human studies suppress infection without toxicity. Covalent binding the catheter surfaces improves efficacy but so far has been implemented using multistep wet...