A5041172802- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Lipid Membrane Structure and Behavior
- Urologic and reproductive health conditions
- Prostate Cancer Diagnosis and Treatment
- Nanopore and Nanochannel Transport Studies
- Prostate Cancer Treatment and Research
- Force Microscopy Techniques and Applications
- Ion Channels and Receptors
- Urological Disorders and Treatments
- Lipid metabolism and biosynthesis
- Magnetic and Electromagnetic Effects
- Neurobiology and Insect Physiology Research
- Genital Health and Disease
- Streptococcal Infections and Treatments
- Peroxisome Proliferator-Activated Receptors
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Function and Risk Factors
- Electrowetting and Microfluidic Technologies
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Chemical and Physical Studies
- Cellular Mechanics and Interactions
Victor Chang Cardiac Research Institute
2013-2023
St Vincent's Hospital Sydney
2012
The University of Queensland
1997-2011
Royal Brisbane and Women's Hospital
2000
QIMR Berghofer Medical Research Institute
1997-1999
Queensland University of Technology
1999
Cancer Council Queensland
1999
Abstract Mechanosensitive ion channels are force-transducing enzymes that couple mechanical stimuli to flux. Understanding the gating mechanism of mechanosensitive is challenging because stimulus seen by channel reflects forces shared between membrane, cytoskeleton and extracellular matrix. Here we examine whether PIEZO1 activated force-transmission through bilayer. To achieve this, generate HEK293 cell membrane blebs largely free cytoskeleton. Using bacterial MscL, calibrate bilayer tension...
Lipid homeostasis is controlled by the peroxisome proliferator-activated receptors (PPARalpha, -beta/delta, and -gamma) that function as fatty acid-dependent DNA-binding proteins regulate lipid metabolism. In vitro in vivo genetic pharmacological studies have demonstrated PPARalpha regulates catabolism. contrast, PPARgamma conflicting process of storage. However, relatively little known about PPARbeta/delta context target tissues, genes, homeostasis, functional overlap with -gamma....
TRP channels of the transient receptor potential ion channel superfamily are involved in a wide variety mechanosensory processes, including touch sensation, pain, blood pressure regulation, bone loading and detection cerebrospinal fluid flow. However, many instances it is unclear whether primary transducers mechanical force these processes. In this study, we tested stretch activation eleven from six mammalian subfamilies. We found that were insensitive to short membrane stretches cellular...
The mechanosensitive channel of large conductance, which serves as a model system for channels, has previously been crystallized in the closed form, but not open form. Ensemble measurements and electrophysiological sieving experiments show that open-diameter pore is >25 Å, exact size whether conformational change follows helix-tilt or barrel-stave are unclear. Here we report distance changes on liposome-reconstituted MscL transmembrane α-helices, using ‘virtual sorting’ single-molecule...
Abstract The bacterial mechanosensitive channel MscL gates in response to membrane tension as a result of mechanical force transmitted directly the from lipid bilayer. represents an excellent model system study basic biophysical principles mechanosensory transduction. However, understanding essential structural components that transduce bilayer into gating remains incomplete. Here using multiple experimental and computational approaches, we demonstrate amphipathic N-terminal helix acts...
Intracellular compartments are functional units that support the metabolism within living cells, through spatiotemporal regulation of chemical reactions and biological processes. Consequently, as a step forward in bottom-up creation artificial building analogous intracellular architectures is essential for expansion cell-mimicking functionality. Herein, we report development droplet laboratory platform to engineer complex emulsion-based, multicompartment using microfluidics acoustic...
Recent studies have identified the liver X receptors (LXRα and LXRβ) as important regulators of cholesterol lipid metabolism. Although originally liver-enriched transcription factors, LXRs are also expressed in skeletal muscle, a tissue that accounts for ∼40% human total body weight is major site glucose utilization fatty acid oxidation. Nevertheless, no yet addressed functional role muscle. In this work we utilize combination vivoand vitro analysis to demonstrate can functionally regulate...
Abstract The droplet on hydrogel bilayer (DHB) is a novel platform for investigating the function of ion channels. Advantages this setup include tight control all components, which compelling investigation mechanosensitive (MS) channels, since they are highly sensitive to their lipid environment. However, activation MS channels in planar supported bilayers, such as DHB, has not yet been established. Here we present large conductance channel E. coli , (MscL), DHBs. By selectively stretching...
Based on sequence similarity, the sp7 gene product, MscSP, of sulfur-compound-decomposing Gram-negative marine bacterium Silicibacter pomeroyi belongs to family MscS-type mechanosensitive channels. To investigate MscSP channel properties, we measured its response membrane tension using patch-clamp technique either a heterologous expression system giant spheroplasts MJF465 Escherichia coli strain (devoid channels MscL, MscS, and MscK), or purified protein reconstituted in azolectin liposomes....
No AccessJournal of UrologyInvestigative Urology1 Apr 1999MOLECULAR DETECTION OF PROSTATE CELLS IN EJACULATE AND URETHRAL WASHINGS MEN WITH SUSPECTED CANCER JUDITH A. CLEMENTS, PAUL ROHDE, VICTORIA ALLEN, VALENTINE J. HYLAND, M.L.T. HEMA SAMARATUNGA, WAYNE D. TILLEY, MARTIN F. LAVIN, and ROBERT GARDINER CLEMENTSJUDITH CLEMENTS More articles by this author , ROHDEPAUL ROHDE ALLENVICTORIA ALLEN HYLANDVALENTINE HYLAND SAMARATUNGAM.L.T. SAMARATUNGA TILLEYWAYNE TILLEY LAVINMARTIN LAVIN...
Activity of the bacterial mechanosensitive channels small conductance MscS/MscK E. coli was investigated under high hydrostatic pressure (HHP) using “flying-patch” patch-clamp technique. The were gated by negative pipette voltage and their open probability measured at HHP 0.1 to 80 MPa. channel decreased with increasing HHP. When osmolyte methylamine N-oxide (TMAO) applied cytoplasmic side inside-out excised membrane patches giant spheroplasts inhibitory effect on activity suppressed...
Xenon is frequently used as a general anesthetic in humans, but the mechanism remains an issue of debate. While for some membrane proteins, direct interaction xenon with protein has been shown to be inhibitory mechanism, other functions could affected by changes properties due partitioning gas into lipid bilayer. Here, effect on mechanosensitive ion channel and copper ion-translocating ATPase was compared under different conditions. inhibited spontaneous gating Escherichia coli...