A5064357764- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- Education Systems and Policy
- Neuroscience and Neuropharmacology Research
- Spectroscopy and Quantum Chemical Studies
- Receptor Mechanisms and Signaling
- Higher Education and Employability
- Electron Spin Resonance Studies
- Solid-state spectroscopy and crystallography
- Youth Education and Societal Dynamics
- Photosynthetic Processes and Mechanisms
- Molecular spectroscopy and chirality
- Neuropeptides and Animal Physiology
- Erythrocyte Function and Pathophysiology
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- Retirement, Disability, and Employment
- Labor market dynamics and wage inequality
- Antimicrobial Peptides and Activities
- Career Development and Diversity
- Higher Education Learning Practices
- Metabolomics and Mass Spectrometry Studies
University of Oxford
2016-2025
Oxfam
2016
University of Derby
2006-2014
East China Normal University
2013
Guidance (United Kingdom)
2006-2012
Centre for Life
1995-2010
London Centre for Nanotechnology
2009
University College London
2009
Rutherford Appleton Laboratory
2006-2007
University of Warwick
1998-2006
Cell surface molecules of eukaryotic cells have been considered to be integrated into the membrane bilayer by a transmembrane protein sequence. The Thy-1 antigen rodent thymocytes and brain was first molecule for which biochemical data clearly suggested integration via nonprotein tail. Direct evidence is now presented showing that glycophospholipid structure attached carboxyl-terminal cysteine residue 31 amino acids predicted from complementary DNA sequence are not present in mature...
Membrane model systems consisting of phosphatidylcholines and hydrophobic α-helical peptides with tryptophan flanking residues, a characteristic motif for transmembrane protein segments, were used to investigate the contribution tryptophans peptide−lipid interactions. Peptides different lengths at positions in sequence incorporated relatively thick or thin lipid bilayers. The organization was assessed by NMR methods hydrogen/deuterium exchange combination mass spectrometry. Previously, it...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTitration of the phase transition phosphatidylserine bilayer membranes. Effects pH, surface electrostatics, ion binding, and head-group hydrationGregor Cevc, Anthony Watts, Derek MarshCite this: Biochemistry 1981, 20, 17, 4955–4965Publication Date (Print):August 18, 1981Publication History Published online1 May 2002Published inissue 18 August 1981https://pubs.acs.org/doi/10.1021/bi00520a023https://doi.org/10.1021/bi00520a023research-articleACS...
The existence of distinct regions mismatch in molecular packing at the interfaces fluid and ordered domains during phase transition dimyristoylphosphatidylcholine vesicles has been demonstrated by measuring temperature dependence permeability to a spin-label cation comparing this with statistical mechanical calculation fraction interfacial lipid. kinetics uptake release 2,2,6,6-tetramethylpiperidinyl-1-oxycholine (Tempo-choline) spin label single-bilayer were measured using electron...
SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, structural dynamic integrity bR was retained when compared data for obtained in native membrane detergents then crystal data. This indicates potential Lipodisq nanoparticles as a useful mimetic.
Lipodisq particles are polymer–lipid complexes formed by detergent-free methods. containing dimyristoylphosphatidylcholine (DMPC) characterized increased lipid ordering compared to a DMPC dispersion. The styrene and maleic acid groups of the polymer interact with chains in bilayers, as well headgroups periphery (see picture). Detailed facts importance specialist readers published "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpin-label studies of lipid immobilization in dimyristoylphosphatidiylcholine-substituted cytochrome oxidasePeter F. Knowles, Anthony Watts, and Derek MarshCite this: Biochemistry 1979, 18, 21, 4480–4487Publication Date (Print):October 16, 1979Publication History Published online1 May 2002Published inissue 16 October 1979https://pubs.acs.org/doi/10.1021/bi00588a005https://doi.org/10.1021/bi00588a005research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRhodopsin-lipid associations in bovine rod outer segment membranes. Identification of immobilized lipid by spin-labelsAnthony Watts, Igor D. Volotovski, and Derek MarshCite this: Biochemistry 1979, 18, 22, 5006–5013Publication Date (Print):October 1, 1979Publication History Published online1 May 2002Published inissue 1 October 1979https://doi.org/10.1021/bi00589a031RIGHTS & PERMISSIONSArticle Views115Altmetric-Citations135LEARN ABOUT THESE...
Antimicrobial peptides are postulated to disrupt microbial phospholipid membranes. The prevailing molecular model is based on the formation of stable or transient pores although direct observation fundamental processes lacking. By combining rational peptide design with topographical (atomic force microscopy) and chemical (nanoscale secondary ion mass spectrometry) imaging same samples, we show that formed by antimicrobial in supported lipid bilayers not necessarily limited a particular...
The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step solubilize isolate proteins. Exposure detergents, however, can destabilize many result in a loss function. Amphipathic copolymers have recently been used stabilize following suitable detergent extraction. However, the ability these extract...
Proton-coupled oligopeptide transporters belong to the major facilitator superfamily (MFS) of membrane transporters. Recent crystal structures suggest MFS fold facilitates transport through rearrangement their two six-helix bundles around a central ligand binding site; how this is achieved, however, poorly understood. Using modeling, molecular dynamics, crystallography, functional assays, and site-directed spin labeling combined with double electron-electron resonance (DEER) spectroscopy, we...
G protein-coupled receptors (GPCRs) are the largest class of membrane receptors, playing a key role in regulation processes as varied neurotransmission and immune response. Evidence for GPCR oligomerisation has been accumulating that challenges idea GPCRs function solely monomeric receptors; however, remains controversial primarily due to difficulties comparing evidence from very different types structural dynamic data. Using combination single-molecule ensemble FRET, double...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization of dimyristoylphosphatidylcholine vesicles and their dimensional changes through the phase transition: molecular control membrane morphologyAnthony Watts, Derek Marsh, Peter F. KnowlesCite this: Biochemistry 1978, 17, 9, 1792–1801Publication Date (Print):May 2, 1978Publication History Published online1 May 2002Published inissue 2 1978https://pubs.acs.org/doi/10.1021/bi00602a034https://doi.org/10.1021/bi00602a034research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. I. Evidence from deuterium NMR measurementsPaul J. R. Spooner and Anthony WattsCite this: Biochemistry 1991, 30, 16, 3871–3879Publication Date (Print):April 23, 1991Publication History Published online1 May 2002Published inissue 23 April 1991https://pubs.acs.org/doi/10.1021/bi00230a010https://doi.org/10.1021/bi00230a010research-articleACS PublicationsRequest reuse...