A5036172001- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Axon Guidance and Neuronal Signaling
- Viral Infections and Vectors
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- Biochemical and Molecular Research
- Viral Infections and Outbreaks Research
- Cell Adhesion Molecules Research
- Viral gastroenteritis research and epidemiology
- Metal-Catalyzed Oxygenation Mechanisms
- HIV Research and Treatment
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Mosquito-borne diseases and control
- Protein Structure and Dynamics
- Wnt/β-catenin signaling in development and cancer
- Microbial metabolism and enzyme function
- Pancreatic function and diabetes
Centre for Human Genetics
2015-2024
University of Oxford
2015-2024
Cancer Research UK
2002-2020
Google (United States)
2016
Johns Hopkins University
2005
Rigshospitalet
2004
Aarhus University Hospital
2004
Copenhagen University Hospital
2004
Medical Research Council
1999-2004
MRC Laboratory of Molecular Biology
1987-2000
Crystallization trials at the Division of Structural Biology in Oxford are now almost exclusively carried out using a high-throughput workflow implemented Protein Production Facility. Initial crystallization screening is based on nanolitre-scale sitting-drop vapour-diffusion experiments (typically 100 nl protein plus reservoir solution per droplet) which use standard kits and 96-well plates. For 294 K barcoded plates entered into an automated storage system with fully integrated imaging...
Enveloped viruses have developed various adroit mechanisms to invade their host cells. This process requires one or more viral envelope glycoprotein achieve cell attachment and membrane fusion. Members of the Flaviviridae such as flaviviruses possess only glycoprotein, E, whereas pestiviruses hepacivirus encode two glycoproteins, E1 E2. Although E2 is involved in attachment, it has been unclear which protein responsible for We report crystal structures homodimeric from pestivirus bovine...
Despite significant progress in high-throughput methods macromolecular crystallography, the production of diffraction-quality crystals remains a major bottleneck. By recording diffraction situ from their crystallization plates at room temperature, number problems associated with crystal handling and cryoprotection can be side-stepped. Using dedicated goniometer installed on microfocus crystallography beamline I24 Diamond Light Source, have been studied an intense flexible beam, allowing...
FLRTs are broadly expressed proteins with the unique property of acting as homophilic cell adhesion molecules and heterophilic repulsive ligands Unc5/Netrin receptors. How these functions direct behavior molecular mechanisms involved remain largely unclear. Here we use X-ray crystallography to reveal distinct structural bases for FLRT-mediated repulsion in neurons. We apply this knowledge elucidate FLRT during cortical development. show that regulate both radial migration pyramidal neurons,...
Wnt signalling regulates multiple processes including angiogenesis, inflammation, and tumorigenesis. Norrin (Norrie Disease Protein) is a cystine-knot like growth factor. Although unrelated to Wnt, activates the Wnt/β-catenin pathway. Signal complex formation involves Frizzled4 (Fz4), low-density lipoprotein receptor related protein 5/6 (Lrp5/6), Tetraspanin-12 glycosaminoglycans (GAGs). Here, we report crystallographic small-angle X-ray scattering analyses of in with Fz4 cysteine-rich...