A5032441791- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Ion Channels and Receptors
- Blood properties and coagulation
- Plant and Biological Electrophysiology Studies
- Bacterial Genetics and Biotechnology
- Cardiac electrophysiology and arrhythmias
- Microfluidic and Bio-sensing Technologies
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Protein Structure and Dynamics
- Chemical and Physical Studies
- RNA and protein synthesis mechanisms
- Hemoglobin structure and function
- Magnetic and Electromagnetic Effects
- Spaceflight effects on biology
- Mass Spectrometry Techniques and Applications
- Yersinia bacterium, plague, ectoparasites research
- Protist diversity and phylogeny
- Electromagnetic Fields and Biological Effects
Victor Chang Cardiac Research Institute
2016-2025
UNSW Sydney
2014-2023
St Vincent's Clinic
2014-2023
St Vincent's Health
2022-2023
University of Chicago
2007-2023
EMBL Australia
2020
The University of Queensland
2005-2014
St Vincent's Hospital Sydney
2012-2014
Institute of Molecular Biology and Biophysics
2012
University of Guelph
2010
We have used the patch-clamp electrical recording technique on giant spheroplasts of Escherichia coli and discovered pressure-activated ion channels. The channels following properties: activation by slight positive or negative pressure; voltage dependence; large conductance; selectivity for anions over cations; dependence activity species permeant ions. believe that these may be involved in bacterial osmoregulation osmotaxis.
Abstract Mechanosensitive ion channels are force-transducing enzymes that couple mechanical stimuli to flux. Understanding the gating mechanism of mechanosensitive is challenging because stimulus seen by channel reflects forces shared between membrane, cytoskeleton and extracellular matrix. Here we examine whether PIEZO1 activated force-transmission through bilayer. To achieve this, generate HEK293 cell membrane blebs largely free cytoskeleton. Using bacterial MscL, calibrate bilayer tension...
Mechanosensitive ion channels use mechanical energy to gate the dissipation of electrochemical gradients across cell membranes. This function is fundamental physiological processes such as hearing and touch. In electrophysiological studies in plasma membrane yeast Saccharomyces cerevisiae , were observed that activated by, adapted to, stretching membrane. Adaptation channel activity stimuli was voltage-dependent. Because these mechanosensitive pass both cations anions, they may play a role...
TRP channels of the transient receptor potential ion channel superfamily are involved in a wide variety mechanosensory processes, including touch sensation, pain, blood pressure regulation, bone loading and detection cerebrospinal fluid flow. However, many instances it is unclear whether primary transducers mechanical force these processes. In this study, we tested stretch activation eleven from six mammalian subfamilies. We found that were insensitive to short membrane stretches cellular...
Mechanosensing is an integral part of many physiological processes including stem cell differentiation, fibrosis, and cancer progression. Two major mechanosensing systems-focal adhesions mechanosensitive ion channels-can convert mechanical features the microenvironment into biochemical signals. We report here unexpectedly that calcium-permeable channel Piezo1, previously perceived to be diffusive on plasma membranes, binds matrix in a force-dependent manner, promoting spreading, adhesion...
The large mechanosensitive ion channel (MscL) of Escherichia coli was expressed on a plasmid encoding MscL as fusion protein with glutathione S-transferase in an strain containing disruption the chromosomal mscL gene. After purification using glutathione-coated beads, thrombin cleavage allowed recovery protein. purified reconstituted into artificial liposomes and found to be fully functional when examined patch-clamp technique. recombinant formed channels that exhibited characteristic...
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in protection bacterial cells against hypoosmotic shock. Although a great deal is known about structure function these channels, much less how membrane lipids may influence their mechanosensitivity function. In this study, we use liposome coreconstitution to examine effects different types on MscS MscL simultaneously using patch-clamp technique confocal microscopy. Fluorescence lifetime imaging...