A5079061656- RNA and protein synthesis mechanisms
- Hematopoietic Stem Cell Transplantation
- Nanoplatforms for cancer theranostics
- Acute Myeloid Leukemia Research
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cancer, Stress, Anesthesia, and Immune Response
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Immune Response and Inflammation
- Corneal Surgery and Treatments
- Traditional Chinese Medicine Studies
- MicroRNA in disease regulation
- Biochemical and Structural Characterization
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Lipid Membrane Structure and Behavior
- Advanced Nanomaterials in Catalysis
- Cancer-related molecular mechanisms research
- Luminescence and Fluorescent Materials
- Genomics and Phylogenetic Studies
- Mesenchymal stem cell research
- Ocular Surface and Contact Lens
- Retinoids in leukemia and cellular processes
- Effects of Radiation Exposure
Nanjing University
2021-2025
Taizhou University
2025
Yan'an Hospital Affiliated To Kunming Medical University
2018-2024
Kunming Medical University
2018-2024
Shanxi Medical University
2024
Chinese PLA General Hospital
2024
First People's Hospital of Chongqing
2023-2024
Institute of Microbiology
2024
Zhejiang University
2017-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
The ability to genetically encode noncanonical amino acids (ncAAs) has empowered proteins with improved or previously unknown properties. However, existing strategies in mammalian cells rely on the introduction of a blank codon incorporate ncAAs, which is inefficient and limits their widespread applications. In this study, we developed rare recoding strategy that takes advantage relative rarity TCG achieve highly selective efficient ncAA incorporation through systematic engineering big...
Abstract The Hippo pathway regulates cell proliferation, apoptosis, and stem self-renewal, its inactivation in animal models causes organ enlargement followed by tumorigenesis. deregulation occurs many human cancers, but the underlying mechanisms are not fully understood. Here, we report tyrosine phosphorylation of tumor suppressor LATS1 as a mechanism regulation adhesion. A kinase library screen identified Src to directly phosphorylate on multiple residues, causing attenuated Mob activator...
Abstract An orthogonal aminoacyl-tRNA synthetase/tRNA pair is a crucial prerequisite for site-specific incorporation of unnatural amino acids. Due to its high codon suppression efficiency and full orthogonality, the pyrrolysyl-tRNA synthetase/pyrrolysyl-tRNA currently ideal system genetic code expansion in both eukaryotes prokaryotes. There pressing need discover or engineer other fully translation systems. Here, through rational chimera design by transplanting key components from...
Aberrant expressions of biomolecules occur much earlier than tumor visualized size and morphology change, but their common measurement strategies such as biopsy suffer from invasive sampling process. In vivo imaging slight biomolecule expression difference is urgently needed for early cancer detection. Fluorescence rare earth nanoparticles (RENPs) in second near-infrared (NIR-II) region makes them appropriate tool imaging. However, the incapacity to couple with signal amplification...
Site-specific incorporation of unnatural amino acids (UAAs) with similar efficiency to that natural (NAAs) and low background activity is extremely valuable for efficient synthesis proteins diverse new chemical functions design various synthetic auxotrophs. However, such translation systems remain largely unknown in the literature. Here, we describe engineered chimeric phenylalanine dramatically increase yield bearing UAAs, through systematic engineering aminoacyl-tRNA synthetase its...
Curcumin is a natural active product extracted from turmeric, has been reported to have anticancer properties against various tumors. However, the effect of curcumin on colon cancer remains still unclear. Our aimed investigate anti-colon in vitro, and determine molecular mechanisms underlying these effects. Viability assays including CCK8 LDH were used measure cell proliferation. Flow cytometry was performed detect apoptosis cycle distribution. qRT-PCR Western blotting for measuring...
Cation-π interactions are the major noncovalent for molecular recognition and play a central role in broad area of chemistry biology. Despite tremendous success understanding origin biological importance cation-π interactions, design synthesis stronger remain elusive. Here, we report an approach that greatly increases binding energy by replacing Trp aromatic box with electron-rich derivative using genetic code expansion strategy. The affinity between histone H3K4me3 its reader is increased...
Despite tremendous success in understanding the chemical nature and importance of cation−π interactions a range biological processes, particularly epigenetic regulation, design synthesis stronger living cells remain largely elusive. Here, we several electron-rich Trp derivatives incorporate them into histone methylation reader domains to enhance affinity for marks via cells. We show that this site-specific replacement strategy is generally applicable engineering high-affinity major H3...
Intravesical instillation is the common therapeutic strategy for bladder cancer. Besides chemo drugs, nanoparticles are used as intravesical reagents, offering appealing approaches cancer treatment. Metal oxide nanoparticle based chemodynamic therapy (CDT) converts tumor intracellular hydrogen peroxide to ROS with cell-specific toxicity, which makes it a promising approach of However, limited penetration agents into mucosa layer wall poses great challenge clinical application CDT in...
Customized cancer therapy relies on timely therapeutic effect evaluation to provide prescription adjustment for individual case. However, currently reported reagents are rarely integrated with imaging probe self-evaluation....
Hypertension-related Depression (HD) is a complex mental disorder that exerts significant negative impact on patients’ quality of life. Previous studies have demonstrated damages to vascular endothelial and hippocampus are the primary pathological features in HD rats. Under hypertensive conditions, inflammatory cytokines peripheral blood vessels can induce central nervous system inflammation through penetration damaged blood-brain barrier, immune cells, neural pathways, damaging brain...
The outcome of older patients with acute myeloid leukemia (AML) remains unsatisfactory. Recent studies have shown that HLA-mismatched microtransplant could improve outcomes in such patients. To evaluate different age groups among newly diagnosed AML who receive microtransplant. This multicenter clinical study included 185 de novo at 12 centers China, the United States, and Spain Microtransplantation Interest Group. Patients were divided into following 4 groups: 60 to 64 years, 65 69 70 74 75...
Chemodynamic therapy (CDT) is a promising therapeutic modality with transition metal ions and endogenous H2O2 as reagents, but its efficiency impaired by low levels nonregeneration of ions. Most intracellular supplement strategies use oxidases are intensively dependent on oxygen participation. The hypoxia microenvironments solid tumors weaken their performance. Here, we develop near-infrared II light powered nanoamplifier to improve the local level enhance CDT. CPNP-Fc/Pt consists ferrocene...
Abstract The optical nanoprobes with emissions in the second near‐infrared window (NIR‐II, 1000–1700 nm) show low tissue autofluorescence and photon scattering; therefore, they provide high spatial resolution acceptable penetration depth. These advantages make them appropriate for vivo applications, including bioimaging, NIR‐II triggered disease therapy, even on‐site efficacy monitoring. Among various developed fluorescence probes, lanthanide‐doped nanoparticles (LDNPs) exhibit photo...
Here we describe the combination of three-dimensional (3D) printed chip and automated microfluidic droplet-based screening techniques for achieving massively parallel, nanoliter-scale protein crystallization under vapor diffusion mode. We fabricated high-density microwell array chips sitting-drop utilizing advantage 3D-printing technique in producing high-aspect-ratio chips. To overcome obstacle 3D-printed microchips performing long-term reactions caused by their porousness gas permeability...
Aim of the Study: To investigate role microRNA-221 (miR-221) in airway epithelial cell injury asthma and delineate underlying mechanism that may involve with SIRT1. Materials Method: Bronchial cells from patients healthy controls were obtained by bronchoscopic brushing. The miR-221 SIRT1 mRNA level collected detected qRT-CPR. BEAS2B lines cultured vitro. In order to up-regulate SIRT1, mimic pcDNA3.1-SIRT1 vector was transfected into cells, respectively. expression changes after transfection...