A5009653241- interferon and immune responses
- Hippo pathway signaling and YAP/TAZ
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Lipid Membrane Structure and Behavior
- Venous Thromboembolism Diagnosis and Management
- Cerebral Venous Sinus Thrombosis
- Parkinson's Disease Mechanisms and Treatments
- Cell death mechanisms and regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- RNA modifications and cancer
- Cellular transport and secretion
- Immune cells in cancer
- Genomics and Phylogenetic Studies
- Neurological disorders and treatments
- Liver Disease Diagnosis and Treatment
- Microtubule and mitosis dynamics
First Affiliated Hospital of Zhengzhou University
2018-2025
Ningbo No. 2 Hospital
2025
Zhejiang University
2012-2024
Zhengzhou University
2020-2024
Henan Academy of Sciences
2024
Henan Provincial Center for Disease Control and Prevention
2024
Zhejiang International Studies University
2017
Loyola University Medical Center
2016
Wuhan University
1997-2004
University of Oklahoma Health Sciences Center
2000-2003
Green fluorescence protein (GFP)-based resonance energy transfer (FRET) is increasingly used in investigation of inter- and intramolecular interactions living cells. In this report, we present a modified method for FRET quantification cultured cells using conventional microscopy. To reliably measure FRET, three positive control constructs which cyan yellow were linked by peptides 15, 24, or 37 amino acid residues prepared. was detected spectrofluorometer, laser scanning confocal microscope,...
There is still no precise knowledge of the causes progression in patients with acute ischemic stroke (AIS), and we are unable to predict at risk.To explore frequency, predictive factors, prognosis early neurological deterioration (END) AIS.In this prospective multicenter observational study, assessed AIS admitted 18 hospitals Henan, China. We defined END as an increase ⩾2 points total National Institutes Health Stroke Scale (NIHSS) score or ⩾1 point motor items NIHSS within 7 days after...
Abstract The Hippo pathway regulates cell proliferation, apoptosis, and stem self-renewal, its inactivation in animal models causes organ enlargement followed by tumorigenesis. deregulation occurs many human cancers, but the underlying mechanisms are not fully understood. Here, we report tyrosine phosphorylation of tumor suppressor LATS1 as a mechanism regulation adhesion. A kinase library screen identified Src to directly phosphorylate on multiple residues, causing attenuated Mob activator...
The human kinome comprises 538 kinases playing essential functions by catalyzing protein phosphorylation. Annotation of subcellular distribution the greatly facilitates investigation normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map annotated to 10 cellular compartments. 456 epitope-tagged kinases, representing 85% kinome, were expressed in HeLa cells imaged immunofluorescent microscopy under a similar condition. KA revealed kinase family-enriched...
Increasing evidence points toward an essential role for complement activation in the pathogenesis of diabetic kidney disease (DKD). However, precise molecular mechanisms remain unclear, and pathway predominantly contributing to DKD is particular interest. In this study, glomerular proteome, especially profiles proteins, was analyzed biopsies from 40 patients 10 normal controls using laser microdissection-assisted liquid chromatography-tandem mass spectrometry (LMD-LC-MS/MS). The abundances...
Cytosolic RNA/DNA sensing elicits primary defense against viral pathogens. Interferon regulatory factor 3 (IRF3), a key signal mediator/transcriptional of the antiviral-sensing pathway, is indispensible for interferon production and antiviral defense. However, how status IRF3 activation controlled remains elusive. Through functional screen human kinome, we found that mammalian sterile 20-like kinase 1 (Mst1), but not Mst2, profoundly inhibited cytosolic nucleic acid sensing. Mst1 associated...
Objective To evaluate the prognosis values of systemic immune–inflammation index (SII) in non-chronic cerebral venous sinus thrombosis (CVST). Methods patients with CVST, admitted to First Affiliated Hospital Zhengzhou University, were retrospectively identified from January 2013 December 2018. We selected acute/subacute phase database. Functional outcomes evaluated modified Rankin Scale (mRS)—mRS 3–6 as poor and mRS 6 death. The overall survival time was defined date onset death or last...
An enzyme safeguards antiviral recognition machinery to avoid leaking and excessive danger response.
Significance Previous studies have demonstrated that Bam protein plays a critical role promoting early germ-line cell differentiation in the Drosophila ovary. Although its regulation and genetic functions been extensively investigated over last 20 years, biochemical nature of has still remained elusive. Here, we show as an ubiquitin-associated regulates stability CycA. Our study uncovers mechanism by which protein, cooperates with Otu to deubiquitinate stabilize CycA, thereby balancing GSC...
Smad7 is a negative feedback product of TGF-β superfamily signaling and fine tunes plethora pleiotropic responses induced by ligands. However, its noncanonical functions independent remain to be elucidated. Here, we show that activates signal transducers activators transcription 3 (STAT3) in maintaining mouse embryonic stem cell pluripotency manner the receptors, yet dependent on leukemia inhibitory factor (LIF) coreceptor glycoprotein 130 (gp130). directly binds intracellular domain gp130...
Abstract Ubiquitin ligase TRAF6, together with ubiquitin-conjugating enzyme Ubc13/Uev1, catalyzes processive assembly of unanchored K63-linked polyubiquitin chains for TAK1 activation in the IL-1R/TLR pathways. However, what domain and how it functions to enable TRAF6’s processivity are largely uncharacterized. Here, we find TRAF6 coiled-coil (CC) is crucial its processivity. The CC mediates oligomerization ensure efficient long chain assembly. Mutating or deleting impairs Fusion E3...
Acute ischemic stroke (AIS) induces high level of neutrophils, which correlates inversely with patient survival. Pyroptosis induced by gasdermin D (GSDMD) has been shown to have an important role in the pathophysiology several inflammatory disorders. The GSDMD neutrophils after AIS is unknown. Using a middle cerebral artery occlusion (MCAO) mouse model, we identified activation pyroptosis signal, including expression caspase-1/11, GSDMD, and interleukin-1β/18 (IL-1β/18), brain spleen at...
Abstract The carboxyl terminus of Hsc70-interacting protein (CHIP) is pivotal for managing misfolded and aggregated proteins via chaperone networks degradation pathways. In a preclinical rodent model CHIP-related ataxia, we observed that CHIP mutations lead to increased levels phosphodiesterase 9A (PDE9A), whose role in this context remains poorly understood. Here, investigated the molecular mechanisms underlying PDE9A ataxia demonstrated binds PDE9A, facilitating its polyubiquitination...
We report the isolation and characterization of a new line mutant Chinese hamster ovary cells, designated SRD-5, that are resistant to 25HC, potent suppressor cleavage sterol regulatory element-binding proteins (SREBPs) in mammalian cells. In SRD-5 SREBPs cleaved constitutively, generating transcriptionally active nuclear SREBP even presence sterols. Sequence analysis cleavage-activating protein (SCAP) transcripts from cells revealed mutation one SCAP allele results substitution conserved...
Abstract The molecular mechanisms that fine tune TLRs responses need to be fully elucidated. Protein phosphatase-1 (PP1) has been shown important in cell death and differentiation. However, the roles of PP1 TLR-triggered immune response remain unclear. In this study, we demonstrate inhibits activation MAPK NF-κB pathway production TNF-α, IL-6 macrophages triggered by TLR3, TLR4, TLR9 a phosphatase-dependent manner. Conversely, knockdown increases TLRs-triggered signaling proinflammatory...