A5076185124- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Cervical Cancer and HPV Research
- NF-κB Signaling Pathways
- Liver Disease and Transplantation
- Liver physiology and pathology
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cell death mechanisms and regulation
- Drug-Induced Hepatotoxicity and Protection
- Circular RNAs in diseases
- HIV Research and Treatment
- Neurological Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Sphingolipid Metabolism and Signaling
- Reproductive tract infections research
- Hepatitis B Virus Studies
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Peroxisome Proliferator-Activated Receptors
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- Extracellular vesicles in disease
Shandong Normal University
2025
Institute of Physics
2025
University of Chinese Academy of Sciences
2025
Chengdu University of Traditional Chinese Medicine
2021-2023
Southwestern Institute of Physics
2022-2023
Kunming Medical University
2015-2022
Daqing City People's Hospital
2018-2021
Huazhong University of Science and Technology
2021
Harbin Medical University
2021
Sixth Affiliated Hospital of Kunming Medical University
2020
Phosphoinositide 3 kinase/Akt pathway plays an essential role in neuronal survival. However, the cellular mechanisms by which Akt suppresses cell death and protects neurons from apoptosis remain unclear. We previously showed that transient expression of constitutively active inhibits ceramide-induced hybrid motor neuron 1 cells. Here we show stable either or Bcl-2 apoptosis, but only prevents release cytochrome c mitochondria, suggesting regulates at a postmitochondrial level. Consistent...
The sphingomyelin derivative ceramide is a signaling molecule implicated in numerous physiological events.Recently published reports indicate that levels are elevated insulin-responsive tissues of diabetic animals and agents which trigger production inhibit insulin signaling.In the present series studies, short-chain analog C 2 -ceramide inhibited insulin-stimulated glucose transport by ϳ50% 3T3-L1 adipocytes, with similar reductions hormone-stimulated translocation transporter (GLUT4)...
Abstract Liver fibrosis is a progressive pathological process induced by various stimuli and may progress to liver cirrhosis cancer. Forsythiaside A (FA) an active ingredient extracted from traditional Chinese medicine Forsythiae Fructus has prominent hepatoprotective activities. However, the unsatisfactory pharmacokinetic properties restrict its clinical application. In this study, nanocarrier of CD44‐specific ligand Hyaluronic acid (HA)‐modified milk‐derived exosomes (mExo) encapsulated...
Ceramide is an important lipid messenger involved in mediating a variety of cell functions including apoptosis. However, mechanisms responsible for ceramide-induced apoptosis remain unclear. We investigated the possibility that ceramide may decrease antiapoptotic signaling cells by inhibiting Akt kinase activity. Our data show C<sub>2</sub>-ceramide induces HMN1 motor neuron and decreases both basal insulin- or serum-stimulated activity 65–70%. These results are consistent with decreased...
The Ras oncogene regulates cellular proliferation, differentiation, transformation, and survival through multiple downstream signals. signals its effector phosphoinositide 3 (PI3) kinase to the Pak protein (p65 <sup>pak</sup>), but steps from remain be elucidated. PI3 can stimulate small G protein, Rac, a direct activator of Pak, as well Akt proto-oncogene, serine-threonine kinase. We found that activated stimulated whereas dominant negative inhibited activation in transfection assays....
The bacterial pathogens of the genus Yersinia, causative agents plague, septicemia, and gastrointestinal syndromes, use a type III secretion system to inject virulence factors into host target cells. One factor, YopJ, is essential for death infected macrophages can block proinflammatory responses by inhibiting both nuclear factor κB (NF-κB) mitogen-activated protein kinase pathways, which might be important evasion immune response aid in establishing systemic infection. Here, we show that...
Ubc13, a ubiquitin-conjugating enzyme (Ubc), requires the presence of Ubc variant (Uev) for polyubiquitination. Uevs, although resembling in sequence and structure, lack active site cysteine residue are catalytically inactive. The yeast Uev (Mms2) incites noncanonical Lys63-linked polyubiquitination by whereas increased diversity Uevs higher eukaryotes suggests an unexpected complication ubiquitination. In this study, we demonstrate that divergent activities mammalian Ubc13 rely on its...
Liver fibrosis (LF) is an important stage in chronic liver disease development, characterized by hepatic stellate cell (HSC) activation and excessive extracellular matrix deposition. Phillygenin (PHI), active component the traditional Chinese medicine Forsythiae Fructus with a significant anti-inflammatory effect, has been proved to inhibit HSC activation. Macrophages can polarize pro-inflammatory M1 phenotype M2 phenotype, participating LF development. Currently, its many components have...
Liver fibrosis is a chronic pathological process that various pathogenic factors lead to abnormal hyperplasia of hepatic connective tissue, and its main feature the excessive deposition extracellular matrix. However, there are currently no drugs approved for treatment liver fibrosis. Phillygenin (PHI), lignan isolated from Forsythiae Fructus, showed potential anti-inflammatory anti-fibrosis effects but mechanisms remain unknown. In view vital role gut microbiota in development fibrosis, this...
Liver fibrosis is a chronic and progressive disease with complex pathogenesis related to bile acids (BAs) gut microbiota. Forsythiaside A (FTA), isolated from the traditional Chinese medicine Forsythiae Fructus (Lian Qiao), natural hepatoprotective agent. The purpose of this study was investigate protective effect FTA on carbon tetrachloride (CCl4)-induced liver in mice. induced mice by intraperitoneal injection 2 mL/kg CCl4 three times week for 4 weeks. attenuated CCl4-induced mice, which...
Hepatitis C virus (HCV) infection is a major cause of chronic liver disease. Although interferon-free direct-acting antivirals have led to significant advancements in the treatment HCV infection, high genetic variability and emergence acquired drug resistance pose potential threats their effectiveness. In this study, we develop broad-spectrum aptamer-based proteolysis targeting chimera, designated dNS5B, which effectively degrades both pan-genotypic NS5B polymerase drug-resistant mutants...
Host DNA repair enzymes have long been assumed to play a role in HIV replication, and many different factors associated with HIV. In order identify pathways required for infection, we conducted targeted siRNA screen using 232 pools genes repair. Mapping the by effective well-defined revealed that of siRNAs targeting short patch base excision (BER) pathway reduced infection. For six BER enzymes, negative effect mRNA knockdown was rescued expression corresponding cDNA, validating importance...