A5031603333- Advanced Fluorescence Microscopy Techniques
- Pancreatic function and diabetes
- Cellular transport and secretion
- Cell Image Analysis Techniques
- GaN-based semiconductor devices and materials
- Digital Holography and Microscopy
- Diabetes and associated disorders
- Advanced Electron Microscopy Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Lipid Membrane Structure and Behavior
- Optical Coherence Tomography Applications
- Ion Channels and Receptors
- Metabolism, Diabetes, and Cancer
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- ZnO doping and properties
- Diabetes Management and Research
- Nanowire Synthesis and Applications
- Mitochondrial Function and Pathology
- Image Processing Techniques and Applications
- Diabetes Treatment and Management
- Semiconductor Quantum Structures and Devices
- Neuroscience and Neuropharmacology Research
Peking University
2016-2025
Institute of Molecular Medicine
2014-2025
Center for Life Sciences
2013-2025
Chinese Institute for Brain Research
2019-2025
National Center for Nanoscience and Technology
2011-2025
State Key Laboratory of Membrane Biology
2016-2025
Imaging Center
2024-2025
McGovern Institute for Brain Research
2024-2025
Beijing National Laboratory for Molecular Sciences
2013-2024
Beijing Academy of Artificial Intelligence
2021-2024
A wire-shaped flexible dye-sensitized solar cell (WSF-DSSC) without any transparent conducting oxide materials is fabricated. The has a helical twisting structure formed by two fiber-like electrodes (100 μm in diameter). Due to the structure, many opaque such as metal wire can be applied. It found that incident-light-angle dependence of cell's IV output extremely low.
In the conserved autophagy pathway, autophagosomes (APs) engulf cellular components and deliver them to lysosome for degradation. Before fusing with lysosome, APs have close via an unknown mechanism. We previously shown that endocytic Rab5-GTPase regulates AP closure. Therefore, we asked whether ESCRT, which catalyzes scission of vesicles into late endosomes, mediates topologically similar process sealing. Here, show depletion representative subunits from all ESCRT complexes causes defects...
Neuropeptides are key signaling molecules in the endocrine and nervous systems that regulate many critical physiological processes. Understanding functions of neuropeptides vivo requires ability to monitor their dynamics with high specificity, sensitivity, spatiotemporal resolution. However, this has been hindered by lack direct, sensitive, noninvasive tools. We developed a series GRAB (G protein-coupled receptor activation‒based) sensors for detecting somatostatin (SST),...
Abstract β-Cell dysfunction and β-cell loss are hallmarks of type 2 diabetes (T2D). Here, we found that trimethylamine N-oxide (TMAO) at a similar concentration to in could directly decrease glucose-stimulated insulin secretion (GSIS) MIN6 cells primary islets from mice or humans. Elevation TMAO levels impairs GSIS, proportion, glucose tolerance male C57BL/6 J mice. inhibits calcium transients through NLRP3 inflammasome-related cytokines induced Serca2 loss, agonist reversed the effect on...
Two proteins, STIM1 in the endoplasmic reticulum and Orai1 plasma membrane, are required for activation of Ca(2+) release-activated (CRAC) channels at cell surface. How these proteins interact to assemble functional CRAC has remained uncertain. Here, we determine how many molecules form a channel. We engineered several genetically expressed fluorescent tandem multimers fluorescent, constitutively active mutant. The assembled into channels, as seen by rectifying inward currents cytoplasmic...
STIM1 and Orai1 are essential components of Ca(2+) release-activated channels (CRACs). After endoplasmic reticulum store depletion, in the aggregates migrates toward cell periphery to co-localize with on opposing plasma membrane. Little is known about roles different domains protein clustering, migration, interaction, and, ultimately, opening CRAC channels. Here we demonstrate that coiled-coil domain C terminus crucial for its aggregation. Amino acids 425-671 STIM1, which contain a...
Abstract The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR achieves high contrast at expense a holistic view interacting partners and surrounding environment. Thus, we developed fluorescence-assisted diffraction computational tomography (SR-FACT), which combines label-free three-dimensional optical (ODT) with two-dimensional Hessian structured illumination microscopy. ODT module is capable resolving...
Abstract Mitochondrial cristae are the main site for oxidative phosphorylation, which is critical cellular energy production. Upon different physiological or pathological stresses, mitochondrial undergo remodeling to reprogram function. However, how formed, maintained, and remolded still largely unknown due technical challenges of tracking crista dynamics in living cells. Here, using live-cell Hessian structured illumination microscopy combined with transmission electron microscopy, focused...
Cyclooctatetraene-conjugated cyanine dyes represent an effective strategy to improve biocompatibility under light in live-cell fluorescence imaging and analysis of mitochondria.
The human kinome comprises 538 kinases playing essential functions by catalyzing protein phosphorylation. Annotation of subcellular distribution the greatly facilitates investigation normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map annotated to 10 cellular compartments. 456 epitope-tagged kinases, representing 85% kinome, were expressed in HeLa cells imaged immunofluorescent microscopy under a similar condition. KA revealed kinase family-enriched...
Abstract pH-sensitive fluorescent proteins (FPs) are highly advantageous for the non-invasive monitoring of exocytosis events. Superecliptic pHluorin (SEP), a green FP, has been widely used imaging single-vesicle exocytosis. However, docking step cannot be visualized using this since fluorescence signal inside vesicles is too low to observed during process. Among available red FPs, none comparable SEP practical applications due unoptimized pH-sensitivity and brightness or severe photochromic...
The medial entorhinal cortex (MEC) creates a map of local space, based on the firing patterns grid, head-direction (HD), border, and object-vector (OV) cells. How these cell types are organized anatomically is debated. In-depth analysis this question requires collection precise anatomical activity data across large populations neurons during unrestrained behavior, which neither electrophysiological nor previous imaging methods fully afford. Here, we examined topographic arrangement spatially...